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ID   PDG1B      preliminary; circular DNA; PLN; 4439 BP.
XX
AC   X13703;
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DT   02-MAR-1989 (Rel. 49, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Dictyostelium plasmid vector pDG1 - complete.
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KW   cloning vector; plasmid; unidentified reading frame.
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OS   Dictyostelium strain GA11 (slime mould)
OC   Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
OC   Rhizopoda; Eumycetozoa; Dictyostelia; Dictyosteliida;
OC   Dictyosteliidae.
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RN   [1]
RP   1-4439
RC   pDG1
RA   Tanaka Y.;
RT   ;
RL   Submitted (07-DEC-1988) on tape to the EMBL Data Library by:
RL   Tanaka Y., University of Tsukuba, Institute of Biological Sciences,
RL   Tsukuba, Ibaraki 305, Japan.
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RN   [2]
RC   pDG1 from Dictyostelium
RA   Orii H., Tanaka Y., Yanagisawa K.;
RT   "Sequence organisation and gene expression of pGD1, a plasmid
RT   found in a wild isolate of Dietyostelum";
RL   Nucleic Acids Res. 17:1395-1408(1989).
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CC   Data kindly reviewed (14-MAR-1989) by Tanaka Y.
CC   NM (pDG1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Dictyostelium)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Dictyostelium
FT                   -> pDG1 4439bp"
FT   rep_origin      0..0
FT                   /note="ORI Dictyostelium pDG1"
FT   repeat_unit     1..591
FT                   /note="RPT inverted repeat A"
FT   misc_feature    533..540
FT                   /note="SIT cAMP responsive element"
FT   promoter        604..611
FT                   /note="S10 putative"
FT   CDS             743..3463
FT                   /note="GEN Dictyostelium ORF; AA 1-906"
FT   repeat_unit     3848..4439
FT                   /note="RPT inverted repeat A'"
XX
SQ   Sequence 4439 BP; 1720 A; 589 C; 672 G; 1458 T; 0 other;
     caaaagtggc ggccattttt tttttttaaa gataattttg ttatatactc taatgttact
     caataacaat aaaagtagta tattctttaa taacttttta ttttgtatcg attggtgctt
     tgaagttagt ttgaaattaa taattcgata attaaaaaaa aaatcacatt ttctccatat
     taatttttga aaaatggcgg ccgttttttg gtagttcaaa ggttggttcc gaaaatgctt
     atttcacata cgttaataat aaaagttagt gattctttat taacttttta ttgtgatcat
     ttatatgctt actagatttt ttttccgtaa tatcataaaa tcttgatttt cctaatttgg
     tttttttttt ttaaataatt tgactttttg ttagattgta atttttgaaa acatgcccag
     taccatctta tccattttta aaacgagtca catcaatctc ttcgcaacga tctcagaaat
     tcattcacgt ttgtttcagt gttattgaat aacattaagg gacacaaact aatgacgaca
     gaataaaaaa aaaacggccg ccattttttg aaaaaaaaaa tttttctaag tcacaatatg
     tcatattaat aatttaaaaa aatagaaaag acacacaaaa caacccccat tttttcccaa
     ttcatttttt ttcgtttttt ttaattttat ttatttacaa tacatttaaa cacaccatta
     tatatatata gaagttacaa aaatgcaaaa tttagctcct tgggaatcat tctttatgtt
     cgttaatata ttattaagag attatgagcc agtaaagagg agacaacaag gtagtgaatc
     taatgaagtt atgaaataca gctttacagt agatacatta atttgtagag aattatttag
     atcaattctt aaagaaaaaa ccttgggtat tttaggtgat tatttaaata gagaatgcat
     ttttgaaaga acaaagaaat tagtaaagct taattataac gatgacgagt acgattacga
     tttagatgat attttaaaaa tacgcaagaa ttcttcaggt aaattaattg ttgatgatat
     tgatcaggcc atattcataa tagaccattt atcaagaaag gttgattgta aagtatttac
     aaaaaaatca ctagttgggt ttagacacat agaaaaaaca ataacggagg ctggatataa
     aataagagag cgtcgtagca ttggtttaga ttggtataca ttgttaaatg atatacgtac
     atcatgtgca aagcatcgta catttgttgc atttagtaaa tacaggtacg tggacttcat
     cgcaatgtta acagcattcc accaagttaa agctgctaag tccaatgaag aagaacatct
     ttcaactata tacagtttat atccctttgt tgaacataat tttgatgaag ataaagaaga
     tgcagcaaca acaaaacaaa caaccacaac aaccacaaca acaccgcaaa ccagaacaag
     aaaaagacaa gcaacggggg ataatacacc aacaccaaca ccagcaccag caccaaagcc
     aacaacacca aaaccaacac cagcaccaag aaagatatca tcaagaaaaa atggtgggct
     tactaatgtt agagttgatc acatttcagt taataatata ccaacaccta gtgatacaaa
     cgaaagcttg agcccaccct ctacaataat atcctcatct aattctatta aacaatgttt
     ggtagaggtg ttggaaagta aaggtgaaat tagtattgat aaaataaaga gtatatttaa
     ttgtttgcaa aataaacaat atactggcga tttaattgat agcatgtttc aacaaaacaa
     atctgaaaag gtcattacca tttcaagtaa actgtttaat atgtccaata aagtagatta
     tgatgaaatt aatttcgcta aattaaagga tgatattctt tatttatcaa gaaggttaat
     atatgaaaag aacacaaatt tattgatacc aactgaagag ggtgaaggta ttattggatt
     cttgtggtta cctgttgtca atggggcttt aacagcatca gtatatgttt cacaacttgg
     tgcagatgta gatttcaaaa agatttcagc cactgtaagg tttgtccaac tgtgtatatc
     aatgagtgat atcatagggt ttatggagct aagatcacta tcattggatg catttagaag
     tattgctaat gagcttttat atatgtcaga cagaatcctt aatcttgaag cagatctacg
     tacactaaag gatattgtaa tgaaaccaac aaatttaaag aaacatgtta atgttgataa
     tttatttagc aggttatcgc ctgaaggtag taatggtttt gcaaattact tgtatgactt
     tatcagtaat catccatata ttaaaattga taaatctcaa aattcaatta aactaaaaga
     aacaccaaac ccagtacttg tattaacata tgacccaaag gtagtagaac ataaagttgg
     attcctattt cattgtcgtt cagagatttc aaggttcaat gcaggtggta ataaatttat
     tttaaataat attcaacatt cattcacccc aaataatatt ggtgtactat cacaagatcg
     tgaaaatgat cttaaaagaa actactcaat gttaacaagt aacacaagta aactaataac
     aggtacaggt tctggtgaac ataatcttga aagatttatt tcaattacca ttaataatac
     tgcatatgat cttattagag ttgtgttgtt tcgtgatata tccaacggga ttagtatatc
     aaatttaagg gatatattca gagaaaatag tgataatcgt aatagatatt tagagtacct
     tggtaaatca agactaatta gagtattctt tattgcacca tgtttgattc aaatccttga
     agtaaacttt gcagctaccc aacttactgc tgataaaaat tttaatcgtg ctattaaatc
     aattaaaatt agaaatcttt cttatattac aattgatata attgttgggg gtaatgttat
     tgattctatt agaggtgatg caactcaagt tgtacaaatt aatgcttcag agtttagctt
     cagtgtttct tgtctaaaat ttagcgttag tgccacactt gtttcaaaaa agaatttgca
     aaatattagt actatagtgc ttaacaaata taatgaagaa aaatcatatt tgcaatgtgt
     aaaaaagttt agcaagcttt ctaaatcatt tattagaaaa tttacaggta tgaacattaa
     tattaatgcg cttgaagaac ttttattaag tgaagccggg gcatatgaag atgatgacga
     tgatgaaggt gagggtaatg aagatgatga agataatgat gaaaatgaag atgaagatga
     aggtgaaggt gaaggtgata gtagtgaaga tgaagatgaa taattaaata aataaaaatg
     atttaaattt aaaataatat atataactat aaaatatgaa tagattatta gtaaaagaga
     ttaacgaaag gttatttatt gagtttttgc gtgcagcttc gttttatgat actgcagcaa
     gatgcattga taaaacaaaa caagatataa aggcaaaaga aagtataaaa aataaaaagt
     atattaaaaa tataattctt gaaccaagat gaggtccaat attgattcta taatatcaaa
     attgtagcct aatttgtaat ctatatatat atatatatag attatttcct tttaaaaaag
     ctaccctgaa aaacctataa aaacctataa accttcaaaa accttcaaaa ccctgcccat
     ataaataact tagaaaaatt tttttttttc aaaaaacggc ggccgttttt tttttattct
     gtcgtcatta gtttgtgtcc cttaatgtta ttcaataaca ctgaaacaaa cgtgaatgaa
     tttctgagat cgttgcgaag agattgatgt gactcgtttt aaaaatggat aagatggtac
     tgggcatgtt ttcaaaaatt acaatctaac aaaaagtcaa attatttaaa aaaaaaaaac
     caaattagga aaatcaagat tttatgatat tacggaaaaa aaatctagta agcatataaa
     tgatcacaat aaaaagttaa taaagaatca cattctttta ttattaacgt atgtgaaata
     agcattttcg gaaccaacct ttgaactacc aaaaaacggc cgccattttt caaaaattaa
     tatggagaaa atgtgatttt ttttttaatt atcgaattat taatttcaaa ctaacttcaa
     agcaccaatc gatacaaaat aaaaagttat taaagaatat actactttta ttgttattga
     gtaacattag agtatataat gagcatttcc tgataaaaaa aaaatggcgg ccatttttt
//