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ID   PDIRECT    preliminary; circular DNA; SYN; 2967 BP.
XX
AC   IG1633;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pDIRECT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pDIRECT from pBR322
RA   ;
RT   ;
RL   Unpublished (1993).
XX
CC   NM (pDIRECT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pDIRECT 2967bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2967 BP; 726 A; 756 C; 735 G; 750 T; 0 other;
     gggccgaacc agttggatcc tctagagcgg ccgccaccgc ggtggagctc caattcgccc
     tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
     actaaaggga acaaaagctg ggtaccgggc cccccctcga ggtcgacggt atcgataagc
     ttgatatcga attcaactcg ctcgccc
//