back Return to this vector's summary.
ID   PDM1       preliminary; circular DNA; SYN; 3984 BP.
XX
AC   ATCC37357;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pDM1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pDM1 from pAT153 & pEMBL8+
RC   pSP64-f1+ from pEMBL8+ & pSP64
RC   pSP64-f1- from pEMBL8+ & pSP64
RC   pSP65-f1+ from pEMBL8+ & pSP65
RC   pSP65-f1- from pEMBL8+ & pSP65
RC   pPTHi74-Sx from pPTHi4 & pPTHi7
RC   pSPTH from pPTHi74-Sx & pSP62-PL1
RC   pUP47 from pSP64-f1- & pSPTH
RC   pPNTH from pUP47 & linker
RA   Mead D.A., Skorupa E.S., Kemper B.;
RT   "Single stranded DNA SP6 promoter plasmids for engineering mutant
RT   RNAs and proteins: synthesis of a 'stretched' preproparathyroid
RT   hormone";
RL   Nucleic Acids Res. 13:1103-1118(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Contains an easily isolated f1 intergenic region for conversion of any
CC   plasmid to an ssDNA vector (use SalI + AccI + HincII).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDM1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli NM522)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAT153)(pEMBL8+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAT153 AccI 3658bp 653..653
FT                   Klenow:Klenow
FT                   bovine alkaline phosphatase
FT                   2. pEMBL8+ RsaI-RsaI 324bp 1285..1609, phage f1 ori
FT                   -> pDM1 3800bp
FT                   \ [AccI/SalI/HincII/f1 ori/AccI/SalI/HincII]"
FT   -               1..654
FT                   /note="pAT153 1..654 654bp
FT                   AccI = GT^MK AC
FT                   RsaI =    GT^AC"
FT   -               655..978
FT                   /note="pEMBL8+ 1285..1608 324bp
FT                   RsaI = GT^AC
FT                   AccI = GT^MKAC"
FT   -               979..3984
FT                   /note="pAT153 653..3658 3006bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3984 BP; 898 A; 1081 C; 1010 G; 995 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgacatat
     gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc
     aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg
     aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt
     tctcacccgt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag
     ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag
     ggtcataatg tttttggtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
     cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtcttca agaa
//