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ID   PDO184     preliminary; circular DNA; SYN; 9820 BP.
XX
AC   U03460; ATCC87042;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pDO184 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9820
RC   pDO2 from pUC19 & lambda::Tn5 & pNEB193
RC   pDO6 from pDO2
RC   pDO17 from pDO2 & pBR322
RC   pDO19 from pDO17
RC   pDO184 from pK184 & pBR322
RA   Ahmed A.;
RT   "Double origin vectors for isolating bidirectional deletions for DNA
RT   sequence analysis";
RL   Gene 141:71-73(1994).
XX
RN   [2]
RP   1-9820
RC   pDO184
RA   Ahmed A.;
RT   ;
RL   Submitted (12-NOV-1993) by:
RL   Ahmed A., Genetics, Biological Sciences Bldg Rm G502,
RL   University of Alberta, Edmonton, Alberta, T6G 2E1, CANADA.
XX
RN   [3]
RC   pDO184 from pBR322 & lambda & kan gene
RA   Ahmed A.L.;
RT   "Isolation of nested, bidirectional deletions by the use of
RT   exonuclease III on double-origin vectors";
RL   Gene 151:97-101(1994).
XX
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--10.0; HindIII--10.0; XbaI--9.2, 1.0; BglI--2.8, 2.35 (doublet),
CC   1.55, 0.75, 0.28; PstI--4.0, 3.7, 2.5. (ATCC staff)
CC   Plasmid contains the following restriction sites (approximate kb from
CC   nt 1):  BamHI--4.42, 4.77; BglI--1.96, 4.60, 5.32, 5.56, 7.88, 9.42;
CC   EcoRI--4.40; HindIII--4.45; PstI--0.65, 4.44, 8.00; PvuI--0.97, 4.57,
CC   8.13; SmaI--4.41; XbaI--3.45, 4.42. (GenBank/EMBL/DDBJ)
CC   A series of overlapping deletion segments can be generated by
CC   partial digestion with a restriction enzyme not found in the vector,
CC   followed by complete digestion with lambda terminase.
CC   The following
CC   restriction sites do not cut pDO17:  Acc65I AflII ApaI AscI AvrII
CC   Ecl136II KpnI NotI PacI PmeI PmlI SacI SfiI SnaBI SpeI SrfI Sse8387I
CC   SwaI XbaI. [1]
CC   Cosmid vector containing two compatible origins of replication, for
CC   generating bidirectional deletions of large inserts to facilitate
CC   sequencing. Resulting fragments are blunted and circularized by
CC   ligation, and transformants are selected for either ampicillin or
CC   kanamycin resistance. AmpR transformants can be sequenced in the
CC   clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'.
CC   KanR transformants can be sequenced in the counterclockwise direction
CC   using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'. [2]
CC   Growth: 1065 plus ampicillin (50 ug/ml) plus kanamycin (20 ug/ml) 30C
CC   Deposited by: Ahmed A.L.
CC   NCBI gi: 424108
CC   NM (pDO184)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli AA102)
CC   CP ()
CC   FN (cloning)(sequencing)
CC   SE (color)
CC   PA (pBR322)
CC   BR (pDO2)(pDO17)(pDO6)(pDO19)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184, p15A ori/kan gene/MCS/lacZ gene
FT                   2. pBR322, ori/amp gene
FT                   -> pDO184 9820bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-Ecl136II-KpnI-Acc65I-SmaI-XmaI-
FT                   Sse83871-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli p15A"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kan gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 9820 BP; 2277 A; 2646 C; 2615 G; 2282 T; 0 other;
     aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
     tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
     cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
     aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
     aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
     gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
     gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
     tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
     gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
     ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
     ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
     cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
     ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga catcgtctgc
     gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt
     tacgcgtttt gccacgatcg ggtccagttc atgcgcaaca gcgttgaact cttcaatggt
     gacatcacgc agggctggct gctggaagaa acgcgcaccg gtttcgcact gttcacgacg
     ggtgttgtat tcgctgccaa ccagggtacg tttgaagtta ctgttgatga tgacgacagc
     cacacctttg ggcatggaaa ctgctttggt ccccagtgag cggcaatcga tcagcaaggc
     atgatctttc ttgccgagcg cggaaattag ctgatccatg atcccgcagt tacagcctac
     aaactggttt tctgcttcct gaccgttaag cgcgatttgt gcgccgtcca gcggcagatg
     ataaagctgc tgcaatacgg ttccgaccgc gacttccagt gaagcggaag aacttaaccc
     ggcaccctgc ggcacattgc cgtgatcaac catgtccacg ccgccgaagc tgttgttacg
     cagttgcaga tgtttcacca cgccacgaac gtagttagcc cattgatagt tttcatgtgc
     gacaatgggc gcatcgaggg aaaactcgtc gagctgattt tcataatcgg ctgccatcac
     gcgaacttta cggtcatcgc gtggtgcaca actgatcacg gtttgataat caatcgcgca
     gggcagaacg aaaccgtcgt tgtagtcggt gtgttcacca atcaaattca cgcggccagg
     cgcctgaatg gtgtgagtgg cagggtagcc aaatgcgttg gcaaacagag attgtgtttt
     ttctttcaga ctcatttctt acactccgga ttcgcgacgg cctacagcaa cctgggcgat
     gtatggcatc aggttattcg gaatgccttg cggatcttcg cccatatcgc ccgacggatg
     cgcgccaacc gggttgaagt agcgcacgag ggcaatgctc cagtccggct gggctttttg
     cagatcggtg aggatctgtt ccaccatcag cttgcttttg ccgtaagggc tttgcggtgt
     gccggtcggg aagctataat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     cgacagcatc gccagtcact atggcgtgct gctagcttca cgctgccgca agcactcagg
     gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc
     cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     gcgatgataa gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg
     tgctacattt gaagagataa attgcactga aatctagaaa tattttatct gattaataag
     atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac
     cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa
     ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca
     tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg
     catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact
     gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     gcaggaacag gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc
     ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     ggagcctatg gaaaaacggc tttgccttct ttcctgcgtt atcccctgat tctgtggata
     accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
     aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat
     aagctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
     gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc
     atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
     tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
     cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaattcc cccttacacg
     gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
     ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
     tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     ataggggttc cgcgcacatt tccccgaaaa gtgcggtaat gactccaact tattgatagt
     gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg
     acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa
     ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca
     gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac
     gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagac
     tgtcatacgc gtgcgtcata tactgactga aaacgcccgc accgttgaag ctgccagcgc
     gctggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
     gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc
     tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt
     cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa cgtgtcgctg aacaatatga
     agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
     gtgctgaacg aaactcccgc actggcaccc gatggtcagc cgtaccgact gttgatctgc
     ctcgctggcc tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc
     atccctttcg gcataccatt ttatgacggc ggcagagtca taaagcacct cattaccctt
     gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact
     cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa
     aaacagagaa aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct
     ttcttttcag agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg
     ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg
//