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ID   PDO19      preliminary; circular DNA; SYN; 11581 BP.
XX
AC   U03461; ATCC87041;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pDO19 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-11581
RC   pDO2 from pUC19 & lambda::Tn5 & pNEB193
RC   pDO6 from pDO2
RC   pDO17 from pDO2 & pBR322
RC   pDO19 from pDO17
RC   pDO184 from pK184 & pBR322
RA   Ahmed A.;
RT   "Double origin vectors for isolating bidirectional deletions for DNA
RT   sequence analysis";
RL   Gene 141:71-73(1994).
XX
RN   [2]
RP   1-11581
RC   pDO19
RA   Ahmed A.;
RT   ;
RL   Submitted (12-NOV-1993) by:
RL   Ahmed A., Genetics, Biological Sciences Bldg Rm G502,
RL   University of Alberta, Edmonton, Alberta, T6G 2E1, CANADA.
XX
RN   [3]
RC   pDO19 from pBR322 & lambda & kan gene
RA   Ahmed A.L.;
RT   "Isolation of nested, bidirectional deletions by the use of
RT   exonuclease III on double-origin vectors";
RL   Gene 151:97-101(1994).
XX
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--11.5; NdeI--5.5, 4.2, 1.8; PvuII--5.8, 3.15, 0.78 (doublet),
CC   0.66, 0.28; PstI--8.4, 2.1, 0.96. (ATCC staff)
CC   Plasmid contains the following restriction sites (approximate kb from
CC   nt 1):  BamHI--6.86; BglI--6.49, 7.42, 7.65, 9.97, 11.18; EcoRI-3.77;
CC   HindIII--2.25; NdeI--2.96, 4.74, 8.79; PstI--0.65, 1.72, 10.10;
CC   PvuI--5.50, 10.22; SalI--7.14; SmaI--0.93, 4.50. (GenBank/EMBL/DDBJ)
CC   A series of overlapping deletion segments can be generated by
CC   partial digestion with a restriction enzyme not found in the vector,
CC   followed by complete digestion with lambda terminase.
CC   The order of the major features in the plasmid is: ren - P - O -
CC   ori lambda - cII - cro - kanR - cosR/L - ampR - oriP - tetR. [1]
CC   Cosmid vector containing two compatible origins of replication, for
CC   generating bidirectional deletions of large inserts to facilitate
CC   sequencing. Resulting fragments are blunted and circularized by
CC   ligation, and transformants are selected for either ampicillin or
CC   kanamycin resistance. AmpR transformants can be sequenced in the
CC   clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'.
CC   KanR transformants can be sequenced in the counterclockwise direction
CC   using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'. [2]
CC   Growth: 1065 plus ampicillin (50 ug/ml) plus kanamycin (20 ug/ml) 30C
CC   Deposited by: Ahmed A.L.
CC   NCBI gi: 424109
CC   NM (pDO19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli AA102)
CC   CP ()
CC   FN (cloning)(sequencing)
CC   SE (color)
CC   PA (pBR322)
CC   BR (pDO2)(pDO6)(pDO184)(pDO17)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pDO17 remove, 12035bp-
FT                   -> pDO19 11581bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli oriP"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage lambda cos"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kan gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tet gene"
FT   misc_binding    0..0
FT                   /note="MCS AatII-ClaI-NheI-BamHI-SgrAI-SalI"
XX
SQ   Sequence 11581 BP; 2863 A; 3081 C; 2985 G; 2652 T; 0 other;
     aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
     tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
     cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
     aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
     aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
     gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
     gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
     tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
     gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
     ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
     ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
     cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
     ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga tcaggcagca
     gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
     cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     ttgcgccctg agtgcttgcg gcagcgtgaa gcttcaggtg agaacatccc tgcctgaaca
     tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca
     tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga
     atttttgcaa gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca
     ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt
     tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt
     gttaatggtt tcttttttgt gctcatacgt taaatctatc accgcaaggg ataaatatct
     aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga
     ggttgtatgg aacaacgcat aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag
     acagctaaag atctcggcgt atatcaaagc gcgatcaaca aggccattca tgcaggccga
     aagatttttt taactataaa cgctgatgga agcgtttatg cggaagaggt aaagcccttc
     ccgagtaaca aaaaaacaac agcataaata accccgctct tacacattcc agccctgaaa
     attgttatct aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac
     gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag
     ctgtgggcgt tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct
     caatgctgct tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc
     gacaagttgc tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     aaatccagat ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa
     tacagcaaaa atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc
     agatctcgat gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc
     agatctgacc aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg
     gaataaacca atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt
     caaacggtgc aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg
     cggcatgttt ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa
     atcccctaaa acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca
     gggggacaca aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagagaattc
     tggcgaatcc tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat
     tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga
     catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga
     tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg
     ctgggcatgc caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga
     taagtggacc caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa
     accaaaactc gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc
     gccgcacaga tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg
     gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc
     agccagttac tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac
     gaaatccgtc gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag
     gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg
     cagtttgttg catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc
     gagctggttg atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag
     tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg
     cgggccaatg cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat
     atgactgcga gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc
     atgggcggta gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct
     aagttcggac tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg
     ggacgcataa tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca
     gcataaatca ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg
     tctggcgaac ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagca
     cgaacctggt ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat
     atggagttaa aagatgacca tctacattac tgagctaata acaggcctgc tggtaatcgc
     aggccttttt atttggggga gagggaagtc atgaaaaaac taacctttga aattcgatct
     ccagcacatc agcaaaacgc tattcacgca gtacagcaaa tccttccaga cccaaccaaa
     ccaatcgtag taaccattca ggaacgcaac cgcagcttag accaaaacag gaagctatgg
     gcctgcttag gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg
     gctaaatcgc gccagcgctg gctgttttac gcgttttgcc acgatcgggt ccagttcatg
     cgcaacagcg ttgaactctt caatggtgac atcacgcagg gctggctgct ggaagaaacg
     cgcaccggtt tcgcactgtt cacgacgggt gttgtattcg ctgccaacca gggtacgttt
     gaagttactg ttgatgatga cgacagccac acctttgggc atggaaactg ctttggtccc
     cagtgagcgg caatcgatca gcaaggcatg atctttcttg ccgagcgcgg aaattagctg
     atccatgatc ccgcagttac agcctacaaa ctggttttct gcttcctgac cgttaagcgc
     gatttgtgcg ccgtccagcg gcagatgata aagctgctgc aatacggttc cgaccgcgac
     ttccagtgaa gcggaagaac ttaacccggc accctgcggc acattgccgt gatcaaccat
     gtccacgccg ccgaagctgt tgttacgcag ttgcagatgt ttcaccacgc cacgaacgta
     gttagcccat tgatagtttt catgtgcgac aatgggcgca tcgagggaaa actcgtcgag
     ctgattttca taatcggctg ccatcacgcg aactttacgg tcatcgcgtg gtgcacaact
     gatcacggtt tgataatcaa tcgcgcaggg cagaacgaaa ccgtcgttgt agtcggtgtg
     ttcaccaatc aaattcacgc ggccaggcgc ctgaatggtg tgagtggcag ggtagccaaa
     tgcgttggca aacagagatt gtgttttttc tttcagactc atttcttaca ctccggattc
     gcgacggcct acagcaacct gggcgatgta tggcatcagg ttattcggaa tgccttgcgg
     atcttcgccc atatcgcccg acggatgcgc gccaaccggg ttgaagtagc gcacgagggc
     aatgctccag tccggctggg ctttttgcag atcggtgagg atctgttcca ccatcagctt
     gcttttgccg taagggcttt gcggtgtgcc ggtcgggaag ctataatgcg gtagtttatc
     acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
     catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
     gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
     agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
     ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
     catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac
     cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
     cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc
     agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc
     atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     ggttccgcgc acatttcccc gaaaagtgcg gtaatgactc caacttattg atagtgtttt
     atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc
     gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc
     tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgtacgca tgaccggcgg
     cggatttggc ggctgtatcg tcgcgctgat cccggaagag ctggtgcctg ccgtacagca
     acgtgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt acgtttgtaa
     accatcacaa ggagcaggac agtgctgaac gaaactcccg cactggcacc cgatggtcag
     ccgtaccgac tgttgatctg cctcgctggc ctgccgcagt tcttcaacct cccggcgcag
     cttttcgttc tcaatttcag catccctttc ggcataccat tttatgacgg cggcagagtc
     ataaagcacc tcattaccct tgccaccgcc tcgcagaacg ggcattccct gttcctgcca
     gttctgaatg gtacggatac tcgcaccgaa aatgtcagcc agctgctttt tgttgacttc
     cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa aaagcctcgc
     tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa cattaagtta
     tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
     g
//