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ID   PDO6       preliminary; circular DNA; SYN; 8932 BP.
XX
AC   U03463; ATCC87039;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pDO6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8932
RC   pDO2 from pUC19 & lambda::Tn5 & pNEB193
RC   pDO6 from pDO2
RC   pDO17 from pDO2 & pBR322
RC   pDO19 from pDO17
RC   pDO184 from pK184 & pBR322
RA   Ahmed A.;
RT   "Double origin vectors for isolating bidirectional deletions for DNA
RT   sequence analysis";
RL   Gene 141:71-73(1994).
XX
RN   [2]
RP   1-8932
RC   pDO6
RA   Ahmed A.;
RT   ;
RL   Submitted (12-NOV-1993) by:
RL   Ahmed A., Genetics, Biological Sciences Bldg Rm G502,
RL   University of Alberta, Edmonton, Alberta, T6G 2E1, CANADA.
XX
RN   [3]
RC   pDO6 from pBR322 & lambda & kan gene
RA   Ahmed A.L.;
RT   "Isolation of nested, bidirectional deletions by the use of
RT   exonuclease III on double-origin vectors";
RL   Gene 151:97-101(1994).
XX
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--6.6, 2.1; HindIII--5.0, 3.9; SstI--9.0. (ATCC staff)
CC   Plasmid contains the following restriction sites (approximate kb from
CC   nt 1):  BamHI--5.91; BglI--5.73, 7.32, 8.53; EcoRI--3.77, 5.88;
CC   HindIII--2.25, 5.96; KpnI--5.89; PstI--0.65, 1.72, 5.94, 7.45;
CC   SacI--5.88; SmaI--0.93, 4.50, 5.89; XbaI--5.92. (GenBank/EMBL/DDBJ)
CC   A series of overlapping deletion segments can be generated by
CC   partial digestion with a restriction enzyme not found in the vector,
CC   followed by complete digestion with lambda terminase.
CC   The order of the major features in the plasmid is: 5' lacZ -
CC   Ecl136II/MCS/Sse8387I - 3' lacZ - ren - P - O - ori lambda - cII -
CC   cro - kanR - cosR/L - ampR - oriP. [1]
CC   Cosmid vector containing two compatible origins of replication, for
CC   generating bidirectional deletions of large inserts to facilitate
CC   sequencing. Resulting fragments are blunted and circularized by
CC   ligation, and transformants are selected for either ampicillin or
CC   kanamycin resistance. AmpR transformants can be sequenced in the
CC   clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'.
CC   KanR transformants can be sequenced in the counterclockwise direction
CC   using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'. [2]
CC   Growth: 1065 plus ampicillin (50 ug/ml) plus kanamycin (20 ug/ml) 30C
CC   Deposited by: Ahmed A.L.
CC   NCBI gi: 424111
CC   NM (pDO6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli AA102)
CC   CP ()
CC   FN (cloning)(sequencing)
CC   SE (color)
CC   PA (pBR322)
CC   BR (pDO2)(pDO17)(pDO184)(pDO19)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pDO2 remove, 9386bp-
FT                   -> pDO6 8932bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage lambda cos"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kan gene"
FT   misc_binding    0..0
FT                   /note="MCS Ecl136II-SacI-Acc65I-KpnI-AscI-BamHI-
FT                   PacI-XbaI-SalI-PmeI-Sse8387I"
XX
SQ   Sequence 8932 BP; 2349 A; 2287 C; 2248 G; 2048 T; 0 other;
     aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
     tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
     cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
     aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
     aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
     gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
     gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
     tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
     gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
     ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
     ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
     cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
     ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga tcaggcagca
     gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
     cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     ttgcgccctg agtgcttgcg gcagcgtgaa gcttcaggtg agaacatccc tgcctgaaca
     tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca
     tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga
     atttttgcaa gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca
     ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt
     tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt
     gttaatggtt tcttttttgt gctcatacgt taaatctatc accgcaaggg ataaatatct
     aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga
     ggttgtatgg aacaacgcat aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag
     acagctaaag atctcggcgt atatcaaagc gcgatcaaca aggccattca tgcaggccga
     aagatttttt taactataaa cgctgatgga agcgtttatg cggaagaggt aaagcccttc
     ccgagtaaca aaaaaacaac agcataaata accccgctct tacacattcc agccctgaaa
     attgttatct aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac
     gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag
     ctgtgggcgt tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct
     caatgctgct tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc
     gacaagttgc tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     aaatccagat ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa
     tacagcaaaa atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc
     agatctcgat gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc
     agatctgacc aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg
     gaataaacca atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt
     caaacggtgc aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg
     cggcatgttt ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa
     atcccctaaa acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca
     gggggacaca aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagagaattc
     tggcgaatcc tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat
     tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga
     catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga
     tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg
     ctgggcatgc caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga
     taagtggacc caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa
     accaaaactc gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc
     gccgcacaga tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg
     gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc
     agccagttac tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac
     gaaatccgtc gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag
     gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg
     cagtttgttg catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc
     gagctggttg atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag
     tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg
     cgggccaatg cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat
     atgactgcga gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc
     atgggcggta gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct
     aagttcggac tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg
     ggacgcataa tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca
     gcataaatca ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg
     tctggcgaac ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagca
     cgaacctggt ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat
     atggagttaa aagatgacca tctacattac tgagctaata acaggcctgc tggtaatcgc
     aggccttttt atttggggga gagggaagtc atgaaaaaac taacctttga aattcgatct
     ccagcacatc agcaaaacgc tattcacgca gtacagcaaa tccttccaga cccaaccaaa
     ccaatcgtag taaccattca ggaacgcaac cgcagcttag accaaaacag gaagctatgg
     gcctgcttag gtgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     ttcgagctcg gtacccgggg gcgcgccgga tccttaatta agtctagagt cgactgttta
     aacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc ggtaatgact ccaacttatt gatagtgttt tatgttcaga
     taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt
     cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat
     cgcttgctga ttacgtgcag ctttcccttc aggcgtacgc atgaccggcg gcggatttgg
     cggctgtatc gtcgcgctga tcccggaaga gctggtgcct gccgtacagc aacgtgtcgc
     tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta aaccatcaca
     aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga
     ctgttgatct gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt
     ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac
     ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc agttctgaat
     ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt ccattgttca
     ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac
     ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga
     agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg cg
//