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ID   PDR2       preliminary; circular DNA; SYN; 10737 BP.
XX
AC   IG1061;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pDR2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   lambda DR2 from lambda
RC   pDR2 from lambda DR2
RA   Murphy A.J., Kung A.L., Swirski R.A., Schimke R.T.;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (pDR2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(human fibroblast or lymphoblast cells)
CC   CP ()
CC   FN (cloning cDNA)(expression)
CC   SE ()
CC   PA (EBV)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda DR2
FT                   1. pBR322 4361bp, ori/amp gene
FT                   2. EBV, oriP/EBNA-1 gene
FT                   3. RSV, LTR
FT                   4. lambda DR2, cI gene
FT                   5. SV40, tk gene
FT                   6. E. coli 1142bp, hyg gene
FT                   -> pDR2 10737bp"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT XboI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 10737 BP; 2686 A; 2596 C; 3033 G; 2422 T; 0 other;
     gtgaccaata caaaacaaaa gcgcccctcg taccagcgaa gaaggggcag agatgccgta
     gtcaggttta gttcgtccgg cggcggggga tctgtatggt gcactctcag tacaatctgc
     tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
     agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
     caatacactt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     gaaggcaaca gacaggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
     taattgtatt taagtgccta gctcgataca ataaacgcca tttgaccatt caccacattg
     gtgtgcacct ccaagcttgt cgaggaactg aaaaaccaga aagttaactg gtaagtttag
     tctttttgtc ttttatttca ggtcccggat cctctagagt cgacctgcag gcatgcaagc
     ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     ccccaggaag ctcctctgtg tcctcataaa ccctaacctc ctctacttga gaggacattc
     caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
     aggaaattgg gtaggggttt ttcacagacc gctttctaag ggtaatttta aaatatctgg
     gaagtccctt ccactgctgt gttccagaag tgttggtaaa cagcccacaa atgtcaacag
     cagaaacata caagctgtca gctttgcaca agggcccaac accctgctca tcaagaagca
     ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac attttcccca cctgtgtagg
     ttccaaaata tctagtgttt tcatttttac ttggatcagg aacccagcac tccactggat
     aagcattatc cttatccaaa acagccttgt ggtcagtgtt catctgctga ctgtcaactg
     tagcattttt tggggttaca gtttgagcag gatatttggt cctgtagttt gctaacacac
     cctgcagctc caaaggttcc ccaccaacag caaaaaaatg aaaatttgac ccttgaatgg
     gttttccagc accattttca tgagtttttt gtgtccctga atgcaagttt aacatagcag
     ttaccccaat aacctcagtt ttaacagtaa cagcttccca catcaaaata tttccacagg
     ttaagtcctc atttaaatta ggcaaaggaa ttcctcgacc tgcagcccaa gcttggcact
     ggcgccagaa atccgcgcgg tggtttttgg gggtcggggg tgtttggcag ccacagacgc
     ccggtgttcg tgtcgcgcca gtacatgcgg tccatgccca ggccatccaa aaaccatggg
     tctgtctgct cagtccagtc gtggacctga ccccacgcaa cgcccaaaag aataaccccc
     acgaaccata aaccattccc catgggggac cccgtcccta acccacgggg cccgtggcta
     tggcgggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     ttggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     ccttccgtgt ttcagttagc ctcccccatc tcccgatccc cacgagtgct ggggcgtcgg
     tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
     gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
     caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
     gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     gacgtcgcgg tgagttcagg ctttttcata tcaagctgat cttgcggcac gctgttgacg
     ctgttaagcg ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata
     tgcgaagtgg acctgggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat
     gacaagacgc tgggcggggt ttgtgtcatc atagaactaa agacatgcaa atatatttct
     tccggggaca ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca gcccggcggc
     acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     gcacgcggcg catctcgggg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     gctcgcggct cttaccagcg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc ttatcatcga
     taagctgatc ctcacaggcc gcacccagct tttcttccgt tgccccagta gcatctctgt
     ctggtgacct tgaagaggaa gaggaggggt cccgagaatc cccatcccta ccgtccagca
     aaaaggggga cgaggaattt gaggcctggc ttgaggctca ggacgcaaat cttgaggatg
     ttcagcggga gttttccggg ctgcgagtaa ttggtgatga ggacgaggat ggttcggagg
     atggggaatt ttcagacctg gatctgtctg acagcgacca tgaaggggat gagggtgggg
     gggctgttgg agggggcagg agtctgcact ccctgtattc actgagcgtc gtctaataaa
     gatgtctatt gatctctttt agtgtgaatc atgtctgacg aggggccagg tacaggacct
     ggaaatggcc taggagagaa gggagacaca tctggaccag aaggctccgg cggcagtgga
     cctcaaagaa gagggggtga taaccatgga cgaggacggg gaagaggacg aggacgagga
     ggcggaagac caggagcccc gggcggctca ggatcagggc caagacatag agatggtgtc
     cggagacccc aaaaacgtcc aagttgcatt ggctgcaaag ggacccacgg tggaacagga
     gcaggagcag gagcgggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga
     gcaggaggag gggcaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca
     ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
     ggaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga
     gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca
     ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggggc aggaggggca
     ggaggggcag gagcaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca
     ggagcaggag gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca
     ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
     ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
     gcaggagcag gaggaggggc aggagcagga ggggcaggag caggaggtgg aggccggggt
     cgaggaggca gtggaggccg gggtcgagga ggtagtggag gccggggtcg aggaggtagt
     ggaggccgcc ggggtagagg acgtgaaaga gccagggggg gaagtcgtga aagagccagg
     gggagaggtc gtggacgtgg agaaaagagg cccaggagtc ccagtagtca gtcatcatca
     tccgggtctc caccgcgcag gccccctcca ggtagaaggc catttttcca ccctgtaggg
     gaagccgatt attttgaata ccaccaagaa ggtggcccag atggtgagcc tgacgtgccc
     ccgggagcga tagagcaggg ccccgcagat gacccaggag aaggcccaag cactggaccc
     cggggtcagg gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa gcatcgtggt
     caaggaggtt ccaacccgaa atttgagaac attgcagaag gtttaagagc tctcctggct
     aggagtcacg tagaaaggac taccgacgaa ggaacttggg tcgccggtgt gttcgtatat
     ggaggtagta agacctccct ttacaaccta aggcgaggaa ctgcccttgc tattccacaa
     tgtcgtctta caccattgag tcgtctcccc tttggaatgg cccctggacc cggcccacaa
     cctggcccgc taagggagtc cattgtctgt tatttcatgg tctttttaca aactcatata
     tttgctgagg ttttgaagga tgcgattaag gaccttgtta tgacaaagcc cgctcctacc
     tgcaatatca gggtgactgt gtgcagcttt gacgatggag tagatttgcc tccctggttt
     ccacctatgg tggaaggggc tgccgcggag ggtgatgacg gagatgacgg agatgaagga
     ggtgatggag atgagggtga ggaagggcag gagtgatgta acttgttagg agacgccctc
     aatcgtatta aaagccgtgt attcccccgc actaaagaat aaatccccag tagacatcat
     gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc attttcgtcc tcccaacatg
     gggcaattgg gcatacccat gttgtcacgt cactcagctc cgcgctcaac accttctcgc
     gttggaaaac attagcgaca tttacctggt gagcaatcag acatgcgacg gctttagcct
     ggcctcctta aattcaccta agaatgggag caaccagcag gaaaaggaca agcagcgaaa
     attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca ctctactact
     gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg atacagatta
     ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
     ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
     ggatagcata tgctacccag atatagatta ggatagcata tgctatccag atatttgggt
     agtatatgct acccagatat aaattaggat agcatatact accctaatct ctattaggat
     agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
     agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
     agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     agcctatgct acccagatat agattaggat agcatatgct atccagatat ttgggtagta
     tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac
     caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag
     caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat
     tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag
     ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg
     cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt
     ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg
     tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc
     ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt
     gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac
     cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt
     gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc
     cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca
     ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca
     ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac
     taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc
     atggggtggg agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat
     atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta
     ctacccggga agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt
     gctaatgccc tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt
     ggtgtagcct cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg
     taagagcttc agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc
     aaagtctgct ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag
     gatgtcaact acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga
     acagggccca gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgcccc
//