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ID   PDR720     preliminary; circular DNA; SYN; 3925 BP.
XX
AC   IG0039;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pDR720 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pDR206 from lacUV5 promoter
RC   [pDR540 from lacUV5 promoter]
RC   [pDR720 from lacUV5 promoter]
RC   from pKO series, gal gene/tac promoter
RA   Russell D.R., Bennett G.N.;
RT   "Construction and analysis of in vivo activity of E. coli promoter
RT   hybrids and promoter mutants that alter the -35 to -10 spacing";
RL   Gene 20:231-243(1982).
XX
CC   The TRP promoter is induced by 3-beta-indoleacrylic acid (IAA).
CC   old Pharmacia vector.
CC   NM (pDR720)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli C600)
CC   CP ()
CC   FN (expression)(transcription)
CC   SE ()
CC   PA (pKO-1)(pBR322)(galK)
CC   BR (pDR540)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli SmaI-SmaI, trp promoter
FT                   2. pKO-1 SmaI 3980bp 312..312
FT                   -> pDR720 3925bp"
FT   promoter        304..374
FT                   /note="PRO E. coli trp
FT                   from pKO-1, in SmaI site"
FT   CDS             550..1696
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   rep_origin      2162..2162
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             2927..3787
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3925 BP; 981 A; 1010 C; 1011 G; 923 T; 0 other;
     gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg
     aagactatcg caccatcagc cagaaaaccg attttgctgg gtgggctaac gatatccgcc
     tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat
     gccaggacaa cttctggtcc ggtaacgtgc tgagcccggc cagggcaata aagcttactc
     cccatccccc cagtgaattc ccctgttgac aattaatcat cgaactagtt aactagtacg
     cagcttggct gcaggtcgac ggatcccccg ggaattcggg ctgcacgcgc acttttatcc
     gcctctgctg cgctccgcca ccgtacgtaa atttatggtt ggttatgaaa tgctggcaga
     gacccagcga gacctgacag tcagcgatat ccattttcgc gaatccggag tgtaagaaat
     gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca
     caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg
     tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agttgtgcac cacgcgatga
     ccgtaaagtt cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcga
     tgcgcccatt gtcgcacatg aaaactatca atgggctaac tacgttcgtg gcgtggtgaa
     acatctgcaa ctgcgtaaca acagcttcgg cggcgtggac atggtgatca gcggcaatgt
     gccgcagggt gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt
     gcagcagctt tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc
     agaaaaccag tttgtaggct gtaactgcgg gatcatggat cagctaattt ccgcgctcgg
     caagaaagat catgccttgc tgatcgattg ccgctcactg gggaccaaag cagtttccat
     gcccaaaggt gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag
     cgaatacaac acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc
     cctgcgtgat gtcaccattg aagagttcaa cgctgttgcg catgaactgg acccgatcgt
     ggcaaaacgc gtgcgtcata tactgactga aaacgcccgc accgttgaag ctgccagcgc
     gctggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
     gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc
     tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt
     cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga
     agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
     gtgctgacag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtcttc aagaa
//