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ID   PDS1       preliminary; circular DNA; SYN; 3833 BP.
XX
AC   A10359;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pDS1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3833
RC   pDS1 from pBR322
RA   Bujard H., Lanzer M.;
RT   "High repressible sequence for control of expression";
RL   Unpublished (1986).
RL   European Patent No. 0303925-A 6 dated 22-FEB-1989.
RL   F. HOFFMANN-LA ROCHE AG.
XX
CC   NCBI gi: 490681
CC   NM (pDS1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pDS1 3833bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3833 BP; 1010 A; 914 C; 926 G; 983 T; 0 other;
     gaattcctcg aggaattccg gatccggcat catggttcga ccattgaact gcatcgtcgc
     cgtgtcccaa aatatgggga ttggcaagaa cggagaccta ccctggcctc cgctcaggaa
     cgagttcaag tacttccaaa gaatgaccac aacctcttca gtggaaggta aacagaatct
     ggtgattatg ggtaggaaaa cctggttctc cattcctgag aagaatcgac ctttaaagga
     cagaattaat atagttctca gtagagaact caaagaacca ccacgaggag ctcattttct
     tgccaaaagt ttggatgatg ccttaagact tattgaacaa ccggaattgg caagtaaagt
     agacatggtt tggatagtcg gaggcagttc tgtttaccag gaagccatga atcaaccagg
     ccaccttaga ctctttgtga caaggatcat gcaggaattt gaaagtgaca cgtttttccc
     agaaattgat ttggggaaat ataaacttct cccagaatac ccaggcgtcc tctctgaggt
     ccaggaggaa aaaggcatca agtataagtt tgaagtctac gagaagaaag actaacagga
     agatgctttc aagttctctg ctcccctcct aaagctatgc atttttataa gaccatggga
     cttttgctgg ctttagatcc ggccaagctt ggactcctgt tgatagatcc agtaatgacc
     tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     gagaatccaa gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     tattggtgcc cttaaacgcc tggggtaatg actctctaga gctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctccccgaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtctgtcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagc tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caa
//