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ID   PDS3       preliminary; circular DNA; SYN; 4041 BP.
XX
AC   A10360;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pDS3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4041
RC   pDS3 from pBR322
RA   Bujard H., Lanzer M.;
RT   "High repressible sequence for control of expression";
RL   Unpublished (1989).
RL   European Patent No. 0303925-A 7 dated 22-FEB-1989.
RL   F. HOFFMANN-LA ROCHE AG.
XX
CC   NCBI gi: 490682
CC   NM (pDS3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pDS3 4041bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4041 BP; 1052 A; 976 C; 980 G; 1033 T; 0 other;
     cctcgacgtc gacgttaacg gtaccgagct tgtggcagtt taaggcgggc gtcctgcccg
     ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact
     caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     ctttcgtttt atttgatgcc tcaagctcgg tacctcgagg gaattccgga tccggcatca
     tggttcgacc attgaactgc atcgtcgccg tgtcccaaaa tatggggatt ggcaagaacg
     gagacctacc ctggcctccg ctcaggaacg agttcaagta cttccaaaga atgaccacaa
     cctcttcagt ggaaggtaaa cagaatctgg tgattatggg taggaaaacc tggttctcca
     ttcctgagaa gaatcgacct ttaaaggaca gaattaatat agttctcagt agagaactca
     aagaaccacc acgaggagct cattttcttg ccaaaagttt ggatgatgcc ttaagactta
     ttgaacaacc ggaattggca agtaaagtag acatggtttg gatagtcgga ggcagttctg
     tttaccagga agccatgaat caaccaggcc accttagact ctttgtgaca aggatcatgc
     aggaatttga aagtgacacg tttttcccag aaattgattt ggggaaatat aaacttctcc
     cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa aggcatcaag tataagtttg
     aagtctacga gaagaaagac taacaggaag atgctttcaa gttctctgct cccctcctaa
     agctatgcat ttttataaga ccatgggact tttgctggct ttagatccgg ccaagcttgg
     actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     cggttgccgc cgggcgtttt ttattggtga gaatccaaag cttggcgaga ttttcaggag
     ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
     gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
     ctctctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtcttc a
//