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ID   PDW227     preliminary; circular DNA; SYN; 4636 BP.
XX
AC   IG9933;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pDW227 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pDW227)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)(S.cerevisiae)(S.pombe)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pGEM-3)(pON163)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3 PvuII 2867bp 105..105
FT                   2. pON163 ClaI-ClaI 1767bp 5651..7418, URA4 gene
FT                   Klenow:Klenow
FT                   -> pDW227 4634bp"
FT   -               1..104
FT                   /note="pGEM-3 1..104 104bp
FT                   PvuII = CAG^CTG
FT                   ClaI =   AT^CGAT"
FT   -               105..1873
FT                   /note="pON163 5651..7419 1769bp
FT                   ClaI = AT^CG AT
FT                   PvuII =  CAG^CTG"
FT   -               1874..4636
FT                   /note="pGEM-3 105..2867 2763bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII
FT                   XbaI-BamHI-AvaI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   CDS             complement(0..0)
FT                   /note="ANT yeast URA4 gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4636 BP; 1303 A; 1090 C; 1002 G; 1241 T; 0 other;
     gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagcgatag cttgtgatat
     tgacgaaact ttttgacatc taatttattc tgttccaaca ccaatgttta taaccaagtt
     ttatcttgtt tgtctacatg gtattttaca ttcatctaca tacatctttc attggctttg
     tacatagtta tcattacaag tctaaaaaaa ttcactcttt tcttattcaa tgtcaatcca
     agagaaaaga ttgtggtaat gttgtaggag catgtttaat aaattactat agcaaattac
     tttttattcc caaggtgttt atctataata gttaatattt tagtcgctac ataaaatttt
     accaaagagt acttgtatac taattctaaa tgccttctga cataaaacgc ctaggaaaac
     aaacgcaaac aaggcatcga ctttttcaat aaccaaccaa aaaaatttta cattagtctt
     tttttaatgc tgagaaagtc tttgctgata tgccttccaa ccagcttctc tatatctctt
     ggcttcgaca acaggattac gaccagctcc atagactcca cgaccaacaa tgatgatatc
     gctaccgcag tttacaatca cttcttcagg agtacgatat tgctgtccca gcccgtctcc
     tttaacatcc aagccgatac caggggacat agttatgtag tcgctttgaa ggttaggaaa
     tcgacgacca gctataaagc caaagcaaaa atcggtatgc ttctcaaacc attctaaggt
     tttctctgtg taggaaccag tagccaaaga gcctttggaa gacatttcag ccaaaagcaa
     gagaccacgt cccaaaggta aaccaacttc tttgaggcct tgtataatac cctcgcctgg
     cactgtatgg caatttgtga tatgagccca agaagcaatt ttgtacacac cagatgcata
     ttgtagcttg acggtatttc caatgtctgc gaatttgcga tcctcaaaga taagaaaacg
     atgcttttta cctaaggcca ccagtttttc taccatatcc tggtcgaaat cctcgacaac
     gtcaatatgt gtcttgataa cacagacata gggtccaatt ttatctacca attctaagat
     ttcggatttc ttcgtcaaat cgaccgcgac tgacaagttg ctttgctttt cttccatcaa
     agccaacaat tccttggcaa tgggattttt catcccctca gctctagctg aatagctttg
     aaatactcta gcatccataa ctttgctttt aaacctttaa tttcgatcca agcaaaaaag
     aggttcttgg taggacaata cggtaagaaa acacgacatg tgcagagatg ccgacgaagc
     atagttaaac tgggatggta aaatcaatta agaatttata aagacaaaat tgtataagtc
     tctaaaacat cttaattata cctcacagaa ctatctaaaa tatattcaca aagtgcaaac
     attatcatga aaaagaacca ttttaattta aagcaagggc attaaggctt atttacagaa
     tttcttactt ttgtaaagat tataaggctg attatctttt tcaccatgcc aaaaattaca
     caagatagaa tggatgtttg aaattaaacg tgagtataca aacaaataca ctaggtaaat
     cgaaacattt ttttctccat taagtaacaa attcctattt agagaaagaa tgctgagtag
     attaaataat ctatacaaac ttttttaaca caaatgcata catatagcca gtgggatttg
     tagctaagct atcctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     ggcatcagag cagattgtac tgagagtgca ccatatcgac gctctccctt atgcgactcc
     tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     ggtgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt tccggggtgc
     ggaacggcgt taccagaaac tcagaaggtt cgtccaacca aaccgactct gacggcagtt
     tacgagagag atgatagggt ctgcttcagt aagccagatg ctacacaatt aggcttgtac
     atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
     tttaggtgac actata
//