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ID   PDW229     preliminary; circular DNA; SYN; 4069 BP.
XX
AC   IG9934;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pDW229 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pDW229)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)(S.cerevisiae)(S.pombe)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pGEM-3)(pON163)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3 NaeI 2867bp 2595..2595
FT                   2. pON163 EcoRI-EcoRI 1198bp 4427..5625, ARS1
FT                   -> pDW229 4065bp"
FT   -               1..2594
FT                   /note="pGEM-3 1..2594 2594bp
FT                   NaeI = GCC^GGC
FT                   EcoRI =  G^AATTC"
FT   -               2595..3796
FT                   /note="pON163 4427..5628 1202bp
FT                   EcoRI = G^AATT C
FT                   NaeI =     GCC^GGC"
FT   -               3797..4069
FT                   /note="pGEM-3 2595..2867 273bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII
FT                   XbaI-BamHI-AvaI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4069 BP; 1161 A; 913 C; 904 G; 1091 T; 0 other;
     gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagctgcat taatgaatcg
     gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg agatggcgcc caacagtccc
     ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     gcgccggtga tgccaattcg agtctaactc cttaaccact cggacatagt gacttatctg
     acactcattg taaattaata tatatatagg cattttgttt agttaaaggt acttaagtaa
     ttagtataaa cgaaccaatt ttataatcag gaagttaagt gaatggtagc acatgtcgta
     aaaattgtga atttttattg aataatattt taaatacaag cctttctaga ctaggtatac
     tcataaacat atgagcaaaa ggatagagga gattacattg catcttctac aaatttattt
     attgcccttt actgaaaaat taaataatga gtactaaatg ataaaaagcg ctcagtacaa
     gaaagattgc aaaaatattg cattcttcat gaattaaagt tgcatataag gcatattgaa
     agtaatagta ctaaaacagc agttagcgaa aattaataga attatattcg caagacaatt
     gtgacattaa attaaaaaat tgtagaattt ttactatcct ctttaacgcc atgagccttt
     ataaaaaggt taaattagtt ttaacattct ttttttgagt aagagtttac aatttatcaa
     aacctgtgtt attatattca ttagtttcaa tttattagca tctagagaaa aatcaattgg
     cagttacatt gttggaattt atgaagaaaa agaatctaca acggagaata agttgctgat
     cgctttccct aaaattgtat attttgctga gcttattttg acatttcgtt gaagttttct
     ctaattcgca ttcattttaa gtaaaacaat gagaaataaa attacaaaaa atacaaatta
     aaatacaatt tttagctata attatagacg atgcccttgt atcccattct gtctcgcttg
     cccctacttt ttatctttta tataccataa tgaacgctgc cgctactaac cataccccga
     ttttacattt cggactccca aggacgtaca aaatagaaaa ctatagaaaa aaataatcag
     aaaatagcat gtcatctctt tgtaaaacgc gtttgcaaga agaaaggaaa caatggagaa
     gagatcatcc atttgtatgt aaattttagt aaacttgaag aaatcactaa caacttctct
     tacttaggga ttctatgcaa aaccttgtaa atcatctgat ggaggactcg atttaatgaa
     ttggaaggtt ggaattggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
     tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
     gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     taatacataa ccttatgtat catacacata cgatttaggt gacactata
//