back Return to this vector's summary.
ID   PDW232     preliminary; circular DNA; SYN; 5510 BP.
XX
AC   IG1062;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pDW232 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [from none]
RA   Becker D.M., Guarente L.;
RT   "High-efficiency transformation of yeast by electroporation";
RL   Meth. Enzymol. 194:182-187(1991).
XX
RN   [2]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
XX
CC   NM (pDW232)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)(S.cerevisiae)(S.pombe)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3 PvuII 2867bp 105..105
FT                   2. pON163 ClaI-ClaI 1767bp 5651..7418, URA4 gene
FT                   Klenow:Klenow
FT                   -> pDW227 4634bp
FT                   1. pGEM-3 NaeI 2867bp 2595..2595
FT                   2. pON163 EcoRI-EcoRI 1198bp 4427..5625, ARS1
FT                   -> pDW229 4065bp
FT                   1. pGEM-3 BglI-Asp700I 479bp 1294..1773, amp gene
FT                   2. pDW229 Asp700I-HindIII 2300bp
FT                   \ 1773..2595..-..2595..2867..8, ARS1
FT                   3. pDW227 HindIII-BglI 3053bp 8..105..-..105..1294,
FT                   \ URA4 gene
FT                   -> pDW232 5510bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII
FT                   XbaI-BamHI-AvaI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   CDS             complement(0..0)
FT                   /note="ANT yeast URA4 gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
XX
SQ   Sequence 5510 BP; 1607 A; 1215 C; 1123 G; 1565 T; 0 other;
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacag ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     cgcgcgtttc ggtgatgacg gtgaatgagg ccgttgagca ccgccgccgc aaggaatggt
     gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc aattccaacc ttccaattca
     ttaaatcgag tcctccatca gatgatttac aaggttttgc atagaatccc taagtaagag
     aagttgttag tgatttcttc aagtttacta aaatttacat acaaatggat gatctcttct
     ccattgtttc ctttcttctt gcaaacgcgt tttacaaaga gatgacatgc tattttctga
     ttattttttt ctatagtttt ctattttgta cgtccttggg agtccgaaat gtaaaatcgg
     ggtatggtta gtagcggcag cgttcattat ggtatataaa agataaaaag taggggcaag
     cgagacagaa tgggatacaa gggcatcgtc tataattata gctaaaaatt gtattttaat
     ttgtattttt tgtaatttta tttctcattg ttttacttaa aatgaatgcg aattagagaa
     aacttcaacg aaatgtcaaa ataagctcag caaaatatac aattttaggg aaagcgatca
     gcaacttatt ctccgttgta gattcttttt cttcataaat tccaacaatg taactgccaa
     ttgatttttc tctagatgct aataaattga aactaatgaa tataataaca caggttttga
     taaattgtaa actcttactc aaaaaaagaa tgttaaaact aatttaacct ttttataaag
     gctcatggcg ttaaagagga tagtaaaaat tctacaattt tttaatttaa tgtcacaatt
     gtcttgcgat taattgttgc cgggaagcta gagtaagagt tcgccagtta atagtttgcg
     caacgttgtg ccattgctac aggcatcgtg gtgtcacgcc gtcgtttggt atggcttcat
     tcagctccgg tcccaacgat caaggcgagt tacatgatcc ccatgttgtg caaaaaagcg
     gttagctcct tcctgaccat ttccggggtg cggaacggcg ttaccagaaa ctcagaaggt
     tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
     taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
     atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa tacaagcttg
     catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc cggtctccct
     atagtgagtc gtattaattt cgataagcca gcgatagctt gtgatattga cgaaactttt
     tgacatctaa tttattctgt tccaacacca atgtttataa ccaagtttta tcttgtttgt
     ctacatggta ttttacattc atctacatac atctttcatt ggctttgtac atagttatca
     ttacaagtct aaaaaaattc actcttttct tattcaatgt caatccaaga gaaaagattg
     tggtaatgtt gtaggagcat gtttaataaa ttactatagc aaattacttt ttattcccaa
     ggtgtttatc tataatagtt aatattttag tcgctacata aaattttacc aaagagtact
     tgtatactaa ttctaaatgc cttctgacat aaaacgccta ggaaaacaaa cgcaaacaag
     gcatcgactt tttcaataac caaccaaaaa aattttacat tagtcttttt ttaatgctga
     gaaagtcttt gctgatatgc cttccaacca gcttctctat atctcttggc ttcgacaaca
     ggattacgac cagctccata gactccacga ccaacaatga tgatatcgct accgcagttt
     acaatcactt cttcaggagt acgatattgc tgtcccagcc cgtctccttt aacatccaag
     ccgataccag gggacatagt tatgtagtcg ctttgaaggt taggaaatcg acgaccagct
     ataaagccaa agcaaaaatc ggtatgcttc tcaaaccatt ctaaggtttt ctctgtgtag
     gaaccagtag ccaaagagcc tttggaagac atttcagcca aaagcaagag accacgtccc
     aaaggtaaac caacttcttt gaggccttgt ataataccct cgcctggcac tgtatggcaa
     tttgtgatat gagcccaaga agcaattttg tacacaccag atgcatattg tagcttgacg
     gtatttccaa tgtctgcgaa tttgcgatcc tcaaagataa gaaaacgatg ctttttacct
     aaggccacca gtttttctac catatcctgg tcgaaatcct cgacaacgtc aatatgtgtc
     ttgataacac agacataggg tccaatttta tctaccaatt ctaagatttc ggatttcttc
     gtcaaatcga ccgcgactga caagttgctt tgcttttctt ccatcaaagc caacaattcc
     ttggcaatgg gatttttcat cccctcagct ctagctgaat agctttgaaa tactctagca
     tccataactt tgcttttaaa cctttaattt cgatccaagc aaaaaagagg ttcttggtag
     gacaatacgg taagaaaaca cgacatgtgc agagatgccg acgaagcata gttaaactgg
     gatggtaaaa tcaattaaga atttataaag acaaaattgt ataagtctct aaaacatctt
     aattatacct cacagaacta tctaaaatat attcacaaag tgcaaacatt atcatgaaaa
     agaaccattt taatttaaag caagggcatt aaggcttatt tacagaattt cttacttttg
     taaagattat aaggctgatt atctttttca ccatgccaaa aattacacaa gatagaatgg
     atgtttgaaa ttaaacgtga gtatacaaac aaatacacta ggtaaatcga aacatttttt
     tctccattaa gtaacaaatt cctatttaga gaaagaatgc tgagtagatt aaataatcta
     tacaaacttt tttaacacaa atgcatacat atagccagtg ggatttgtag ctaagctatc
     gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
//