Return to this vector's summary.
ID PE194 preliminary; circular DNA; SYN; 3728 BP.
XX
AC V01278; J01755; J01756; J01757; J01758; V01279; V01280;
AC ATCC37128; ATCC68359;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Bacillus/Staphylococcus plasmid vector pE194 - complete.
XX
KW cloning vector.
XX
OS Staphylococcus aureus
OC Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC Micrococcaceae.
XX
RN [1]
RC from pC194 & pE194
RC from pE194
RA Horinouchi S., Weisblum B.;
RT "Posttranscriptional modification of mRNA conformation: mechanism
RT that regulates erythromycin-induced resistance";
RL Proc. Natl. Acad. Sci. U.S.A. 77:7079-7083(1980).
XX
RN [2]
RC pSA0302 from pT181 & pSA0501
RC pSA2200 from pT181 & pSA0501
RC plasmid from pSA2100 & pT181
RC pE194 from S. aureus
RC pSA0301 from pT181
RA Iordanescu S.;
RT "Relationships between cotransducible plasmids in Staphylococcus
RT aureus";
RL J. Bacteriol. 129:71-75(1977).
XX
RN [3]
RP 1723-3074
RC pBD15 from pE194
RC pBD16, pBD73, pBD74 from pBD15
RA Gryczan T.J., Grandi G., Hahn J., Grandi R., Dubnau D.;
RT "Conformational alteration of mRNA structure and the
RT posttranscriptional regulation of erythromycin-induced drug
RT resistance";
RL Nucleic Acids Res. 8:6081-6097(1980).
XX
RN [4]
RP 1-3728
RC pHW1 from pE194 & pC194
RC pHW2 from pE194 & pC194
RC pHW3 from pE194 & B.subtilis DHFR gene
RC pHW4 from pE194 & pBR322
RA Horinouchi S., Weisblum B.;
RT "Nucleotide sequence and functional map of pE194, a plasmid that
RT specifies inducible resistance to macrolide, lincosamide and
RT streptogramin type B antibiotics";
RL J. Bacteriol. 150:804-814(1982).
XX
CC For full induction, the plasmid requires a period of induction by
CC subinhibitory concentrations of erythromycin. [3]
CC Temperature sensitive. [3]
CC Plasmid is a natural isolate from Staphylococcus aureus that confers
CC resistance to macrolide, lincosamide and streptogramin type B 'MLS'
CC antibiotics. [3]
CC Bacillus Genetic Stock Center <-- B. Weisblum <-- B. Iordanescu. (ATCC
CC staff)
CC Restriction digests of the clone give the following sizes (kb):
CC AccI--3.7; PstI--3.7. (ATCC staff)
CC Medium is 1179 V Y medium.
CC NM (pE194)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (B.subtilis BSU1E18)(Bacillus)(Staphylococcus aureus)
CC HO (B.subtilis BD170)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Staphylococcus aureus
FT -> pE194 3728bp"
FT misc_binding 0..0
FT /note="MCS unique BclI-AccI-PstI"
FT rep_origin 0..0
FT /note="ORI Staphylococcus aureus"
FT CDS 0..0
FT /note="ANT Staphylococcus erythromycin resistance gene
FT (ery)"
FT CDS complement(860..1173)
FT /note="GEN Staphylococcus reading frame E"
FT CDS 1244..1591
FT /note="GEN Staphylococcus reading frame"
FT CDS 1506..1856
FT /note="GEN Staphylococcus reading frame C"
FT CDS complement(2007..2738)
FT /note="GEN Staphylococcus reading frame B
FT mls resistance"
FT CDS complement(2801..2857)
FT /note="GEN Staphylococcus reading frame F translation
FT attenuator"
FT CDS join(3150..3728,1..630)
FT /note="GEN Staphylococcus reading frame A (over end
FT of circular sequence)"
XX
SQ Sequence 3728 BP; 1362 A; 460 C; 722 G; 1184 T; 0 other;
gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag
atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
aagcgatg
//