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ID   PE194      preliminary; circular DNA; SYN; 3728 BP.
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AC   V01278; J01755; J01756; J01757; J01758; V01279; V01280;
AC   ATCC37128; ATCC68359;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pE194 - complete.
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KW   cloning vector.
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OS   Staphylococcus aureus
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Micrococcaceae.
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RN   [1]
RC   from pC194 & pE194
RC   from pE194
RA   Horinouchi S., Weisblum B.;
RT   "Posttranscriptional modification of mRNA conformation: mechanism
RT   that regulates erythromycin-induced resistance";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:7079-7083(1980).
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RN   [2]
RC   pSA0302 from pT181 & pSA0501
RC   pSA2200 from pT181 & pSA0501
RC   plasmid from pSA2100 & pT181
RC   pE194 from S. aureus
RC   pSA0301 from pT181
RA   Iordanescu S.;
RT   "Relationships between cotransducible plasmids in Staphylococcus
RT   aureus";
RL   J. Bacteriol. 129:71-75(1977).
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RN   [3]
RP   1723-3074
RC   pBD15 from pE194
RC   pBD16, pBD73, pBD74 from pBD15
RA   Gryczan T.J., Grandi G., Hahn J., Grandi R., Dubnau D.;
RT   "Conformational alteration of mRNA structure and the
RT   posttranscriptional regulation of erythromycin-induced drug
RT   resistance";
RL   Nucleic Acids Res. 8:6081-6097(1980).
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RN   [4]
RP   1-3728
RC   pHW1 from pE194 & pC194
RC   pHW2 from pE194 & pC194
RC   pHW3 from pE194 & B.subtilis DHFR gene
RC   pHW4 from pE194 & pBR322
RA   Horinouchi S., Weisblum B.;
RT   "Nucleotide sequence and functional map of pE194, a plasmid that
RT   specifies inducible resistance to macrolide, lincosamide and
RT   streptogramin type B antibiotics";
RL   J. Bacteriol. 150:804-814(1982).
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CC   For full induction, the plasmid requires a period of induction by
CC   subinhibitory concentrations of erythromycin. [3]
CC   Temperature sensitive. [3]
CC   Plasmid is a natural isolate from Staphylococcus aureus that confers
CC   resistance to macrolide, lincosamide and streptogramin type B 'MLS'
CC   antibiotics. [3]
CC   Bacillus Genetic Stock Center <-- B. Weisblum <-- B. Iordanescu. (ATCC
CC   staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   AccI--3.7; PstI--3.7. (ATCC staff)
CC   Medium is 1179 V Y medium.
CC   NM (pE194)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (B.subtilis BSU1E18)(Bacillus)(Staphylococcus aureus)
CC   HO (B.subtilis BD170)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus aureus
FT                   -> pE194 3728bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BclI-AccI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus aureus"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus erythromycin resistance gene
FT                   (ery)"
FT   CDS             complement(860..1173)
FT                   /note="GEN Staphylococcus reading frame E"
FT   CDS             1244..1591
FT                   /note="GEN Staphylococcus reading frame"
FT   CDS             1506..1856
FT                   /note="GEN Staphylococcus reading frame C"
FT   CDS             complement(2007..2738)
FT                   /note="GEN Staphylococcus reading frame B
FT                   mls resistance"
FT   CDS             complement(2801..2857)
FT                   /note="GEN Staphylococcus reading frame F translation
FT                   attenuator"
FT   CDS             join(3150..3728,1..630)
FT                   /note="GEN Staphylococcus reading frame A (over end
FT                   of circular sequence)"
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SQ   Sequence 3728 BP; 1362 A; 460 C; 722 G; 1184 T; 0 other;
     gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
     ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
     tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
     atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
     gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
     aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
     agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
     tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
     gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
     cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
     gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
     tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
     taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
     gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
     tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
     taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
     ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag
     atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
     tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
     taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
     ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
     gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
     atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
     cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
     aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
     aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
     ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
     tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
     ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
     gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
     actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
     ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
     gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
     atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
     aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
     ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
     taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
     agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
     gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
     ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
     ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
     tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
     acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
     ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
     ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
     ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
     atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
     gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
     gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
     ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
     ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
     cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
     tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
     ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
     ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
     aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
     gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
     tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
     tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
     tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
     aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
     atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
     aagcgatg
//