back Return to this vector's summary.
ID   PEB111     preliminary; circular DNA; SYN; 6415 BP.
XX
AC   IG9888;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pEB111 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKBU5 from pGHS1 & pKBU4
RC   pKBU5M from pKBU5
RC   pKBU6 from pKBU5M & pGKV223
RC   pKBU7 from pKBU5M & pGKV11
RC   pGKV259-2 from pGKV259
RC   pGKH2 from pGKV259-2 & pGKH1
RC   pHPS10 from pGKH2 & pHP13-2
RA   Haima P., Van Sinderen D., Bron S., Venema G.;
RT   "An improved beta-galactosidase alpha-complementation system for
RT   molecular cloning in Bacillus subtilis";
RL   Gene 93:41-47(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pEB111)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pKK223-3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 PvuII-EcoRI 3514bp 1036..4548..2
FT                   :fill in
FT                   2. pKK223-3 remove BamHI-PvuII 1691bp 257..1948, tet
FT                   \ 2893bp
FT                   \ PvuII = 1948
FT                   \ BamHI = 257 4573
FT                   fill in:
FT                   -> pEB111 6407bp"
FT   -               1..260
FT                   /note="pKK223-3 1..260 260bp
FT                   BamHI = G^GATC C
FT                   EcoRI =      G^AATTC"
FT   -               261..3778
FT                   /note="pUB110 1036..4548..5 3518bp complement
FT                   PvuII = CAG^CTG"
FT   -               3779..6415
FT                   /note="pKK223-3 1948..4584 2637bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6415 BP; 1912 A; 1249 C; 1495 G; 1759 T; 0 other;
     ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg
     attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
     aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
     ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     gcagtcgata agcccggatc aattccttaa ggaacgtaca gacggcttaa aagcctttaa
     aaacgttttt aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa
     acacgattta gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa
     agaacgaagt cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt
     ctttttttga tggttttgaa cttgttcttt cttatcttga tacatataga aataacgtca
     tttttatttt agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat
     tgaaaaccct taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacta
     aacaaataac taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt
     attcagatga aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat
     ggagagaaaa gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta
     aaaaggctga aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa
     caggcgaaag aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca
     actggaggag agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta
     aacaaaagcc aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg
     gcgaagaatt aaataagagt ttgtcagata tggctcaagg atttcgccga atgatgcaat
     ataaaaaaat taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata
     ataaagataa ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt
     ttaagaatac agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa
     tgaaattaga ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata
     aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt
     ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac
     accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa aaattaaacc
     ttgatgacac agaagaaggc gatttgattc atacagatga tgacgaaaaa gccgatgaag
     atggattttc tattattgca atgtggaatt gggaacggaa aaattatttt attaaagagt
     agttcaacaa acgggccagt ttgttgaaga ttagatgcta taattgttat taaaaggatt
     gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct ctccaaatat
     tcttatttag aaaagcaaat ctaaaattat ctgaaaaggg aatgagaata gtgaatggac
     caataataat gactagagaa gaaagaatga agattgttca tgaaattaag gaacgaatat
     tggataaata tggggatgat gttaaggcta ttggtgttta tggctctctt ggtcgtcaga
     ctgatgggcc ctattcggat attgagatga tgtgtgtcat gtcaacagag gaagcagagt
     tcagccatga atggacaacc ggtgagtgga aggtggaagt gaattttgat agcgaagaga
     ttctactaga ttatgcatct caggtggaat cagattggcc gcttacacat ggtcaatttt
     tctctatttt gccgatttat gattcaggtg gatacttaga gaaagtgtat caaactgcta
     aatcggtaga agcccaaacg ttccacgatg cgatttgtgc ccttatcgta gaagagctgt
     ttgaatatgc aggcaaatgg cgtaatattc gtgtgcaagg accgacaaca tttctaccat
     ccttgactgt acaggtagca atggcaggtg ccatgttgat tggtctgcat catcgcatct
     gttatacgac gagcgcttcg gtcttaactg aagcagttaa gcaatcagat cttccttcag
     gttatgacca tctgtgccag ttcgtaatgt ctggtcaact ttccgactct gagaaacttc
     tggaatcgct agagaatttc tggaatggga ttcaggagtg gacagaacga cacggatata
     tagtggatgt gtcaaaacgc ataccatttt gaacgatgac ctctaataat tgttaatcat
     gttggttacg tatttattaa cttctcctag tattagtaat tatcatggct gtcatggcgc
     attaacggaa taaagggtgt gcttaaatcg ggccattttg cgtaataaga aaaaggatta
     attatgagcg aattgaatta ataataaggt aatagattta cattagaaaa tgaaagggga
     ttttatgcgt gagaatgtta cagtctatcc cggcattgcc agtcggggat attaaaaaga
     gtataggttt ttattgcgat aaactaggtt tcactttggt tcaccatgaa gatggattcg
     cagttctaat gtgtaatgag gttcggattc atctatggga ggcaagtgat gaagctggcg
     ctctcgtagt aatgattcac cggtttgtac aggtgcggag tcgtttattg ctggtactgc
     tagttgccgc attgaagtag agggaattga tgaattatat caacatatta agcctttggg
     cattttgcac cccaatacat cattaaaaga tcagtggtgg gatgaacgag actttgcagt
     aattgatccc gacaacaatt tgattagctt ttttcaacaa ataaaaagct aaaatctatt
     attaatctgt tcagcaatcg ggcgcgattg ctgaataaaa gatacgagag acctctcttg
     tatctttttt attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa
     ttttattctt gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg
     gcaagggttt aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct
     tgctgatttt taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag
     gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg
     gtcggcactg ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt
     atactttact tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta
     aaaaggagcg atttacatat gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct
     gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acagcatcgt ggtgtcacgc
     tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     tagaaaaata aacaaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc
     gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca
     aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct
     tctctcatcc gccaaaacag ccaagcttgg ctgcaggtcg acggatcccc gggaa
//