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ID   PEBVHISA   preliminary; circular DNA; SYN; 10317 BP.
XX
AC   IG1063; K01383;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pEBVHisA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pcDV1/S2 from pcDV1 & linker
RC   pRSVCAT/H2 from pRSVcat & linker
RC   [pBT from BT gene]
RC   pRSV/BT from pRSVCAT/H2 & pBT
RC   pRSVPA1 from pRSV/BT & pcDV1/S2
RC   pRSVPA2 from pRSVPA1 & linker
RC   p220.2 from p201
RC   p220.2/B- from p220.2
RC   pREP2 from pRSVPA2 & p220.2/B-
RC   pREP3 from pREP2
RC   p220.2/XS from p220.2/B-
RC   pHS1PA4 from pRSVPA2
RC   pMEP4 from p220.2/XS & pHS1PA4
RC   pRSVCAT-B2 from pRSVcat & linker
RC   pRSVCAT-B2K from pRSVCAT-B2 & linker
RC   pREP4 from pRSVCAT-B2K & pMEP4
RC   pREP5 from pREP4 & oligo
RA   Groger R.K., Morrow D.M., Tykocinski M.L.;
RT   "Directional antisense and sense cDNA cloning using Epstein-Barr
RT   virus episomal expression vectors";
RL   Gene 81:285-294(1989).
XX
CC   NM (pEBVHisA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)
CC   BR (pEBVHisB)(pEBVHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EBV, EBNA-1
FT                   2. SV40, polyA/tk gene
FT                   3. RSV, LTR
FT                   4. pBR322 4361bp
FT                   5. E. coli 1142bp, hyg gene
FT                   -> pEBVHisA 10317bp"
FT   misc_binding    1..1
FT                   /note="SIT XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   misc_binding    1..1
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
XX
SQ   Sequence 10317 BP; 2528 A; 2591 C; 2933 G; 2265 T; 0 other;
     tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
     acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
     tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
     gatctcgagc tcggatcccc atcgatcctt atcgtcatcg tcgtacagat cccgacccat
     ttgctgtcca ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat
     ggaggtgcac accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact
     taaatacaat tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg
     tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
     ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
     acaagtgtat tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat
     cctgagggga ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta
     gtcccctcag atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
     cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
     gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
     tgcggcagca gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat
     aagctgtcaa acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt
     tgggggtcgg gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg
     cggtccatgc ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc
     agaccccacg caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg
     gaccccgtcc ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg
     ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc
     gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc
     gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt
     gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc
     catctcccct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
     tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     tcaggctttt tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg
     ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg
     acctgggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc
     tgggcggggt ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca
     ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc
     tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag
     gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     gaattctcat gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag
     cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg
     gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg
     gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt
     aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc
     tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca
     ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa
     tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca
     catctggacc agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg
     gacgaggacg gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct
     caggatcagg gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca
     ttggctgcaa agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag
     caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
     gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
     gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
     gggcaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag
     gaggggcagg agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag
     caggaggagg ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg
     caggagcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
     gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     caggaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
     caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     gaggggcagg agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag
     gaggtagtgg aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa
     gagccagggg gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga
     ggcccaggag tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc
     caggtagaag gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag
     aaggtggccc agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag
     atgacccagg agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca
     aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga
     acattgcaga aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg
     aaggaacttg ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc
     taaggcgagg aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc
     cctttggaat ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct
     gttatttcat ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta
     aggaccttgt tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct
     ttgacgatgg agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg
     agggtgatga cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc
     aggagtgatg taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc
     gcactaaaga ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc
     tgtcttgtca ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac
     gtcactcagc tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg
     gtgagcaatc agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg
     agcaaccagc atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc
     ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct gactgtatat
     gcatgaggat agcatatgct acccggatac agattaggat agcatatact acccagatat
     agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
     aaattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
     agattaggat agcctatgct acccagatat agattaggat agcatatgct acccagatat
     agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat
     taggatagca tatactaccc taatctctat taggatagca tatgctaccc ggatacagat
     taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
     taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
     taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc
     ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca
     ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg
     cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt
     tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc
     caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa
     aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc
     gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt
     gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct
     taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg
     tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga
     tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat
     attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg
     tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt
     tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact
     gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg
     aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc
     cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg
     ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc
     gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc
     ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct
     gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca
     cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc
     actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc
     cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta
     ccaaccaact gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta
     ccagcgaaga aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//