back Return to this vector's summary.
ID   PEBVHISB   preliminary; circular DNA; SYN; 10307 BP.
XX
AC   IG1064; K01383;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pEBVHisB - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pcDV1/S2 from pcDV1 & linker
RC   pRSVCAT/H2 from pRSVcat & linker
RC   [pBT from BT gene]
RC   pRSV/BT from pRSVCAT/H2 & pBT
RC   pRSVPA1 from pRSV/BT & pcDV1/S2
RC   pRSVPA2 from pRSVPA1 & linker
RC   p220.2 from p201
RC   p220.2/B- from p220.2
RC   pREP2 from pRSVPA2 & p220.2/B-
RC   pREP3 from pREP2
RC   p220.2/XS from p220.2/B-
RC   pHS1PA4 from pRSVPA2
RC   pMEP4 from p220.2/XS & pHS1PA4
RC   pRSVCAT-B2 from pRSVcat & linker
RC   pRSVCAT-B2K from pRSVCAT-B2 & linker
RC   pREP4 from pRSVCAT-B2K & pMEP4
RC   pREP5 from pREP4 & oligo
RA   Groger R.K., Morrow D.M., Tykocinski M.L.;
RT   "Directional antisense and sense cDNA cloning using Epstein-Barr
RT   virus episomal expression vectors";
RL   Gene 81:285-294(1989).
XX
CC   NM (pEBVHisB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)
CC   BR (pEBVHisA)(pEBVHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EBV, EBNA-1
FT                   2. SV40, polyA/tk gene
FT                   3. RSV, LTR
FT                   4. pBR322 4361bp
FT                   5. E. coli 1142bp, hyg gene
FT                   -> pEBVHisB 10307bp"
FT   misc_binding    1..1
FT                   /note="SIT XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   misc_binding    1..1
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
XX
SQ   Sequence 10307 BP; 2526 A; 2586 C; 2932 G; 2263 T; 0 other;
     tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
     acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
     tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
     gatctcgagc tcggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
     ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat ggaggtgcac
     accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact taaatacaat
     tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg tctgttgcct
     tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
     ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagtgtat
     tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga
     ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta gtcccctcag
     atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
     ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
     tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcagca
     gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat aagctgtcaa
     acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt tgggggtcgg
     gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc
     ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc agaccccacg
     caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
     ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
     cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccct
     attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg ggtcgctgca
     gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg acctgggacc
     gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc tgggcggggt
     ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa
     acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat
     gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc
     gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
     tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
     caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
     gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
     catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
     tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
     cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
     agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
     gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
     gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
     agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
     aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg
     ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
     agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
     aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
     agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg
     ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg
     aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
     aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg
     ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc
     aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg
     agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg
     aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg
     gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag
     tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag
     gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc
     agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg
     agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg
     gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga
     aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg
     ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg
     aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat
     ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat
     ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt
     tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg
     agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga
     cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg
     taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga
     ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca
     ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc
     tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
     agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
     atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
     aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
     agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
     agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
     agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     agcctatgct acccagatat agattaggat agcatatgct acccagatat agattaggat
     agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat taggatagca
     tatactaccc taatctctat taggatagca tatgctaccc ggatacagat taggatagca
     tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
     tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
     tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
     ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
     tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
     tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
     ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
     gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
     acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
     gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
     gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
     agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
     atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
     gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
     tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
     ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
     gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
     tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
     ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
     ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
     agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
     aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
     ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
     gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
     atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
     ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
     tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
     gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta ccagcgaaga
     aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//