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ID PEBVHISB preliminary; circular DNA; SYN; 10307 BP.
XX
AC IG1064; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pEBVHisB - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pEBVHisB)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR (pEBVHisA)(pEBVHisC)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. EBV, EBNA-1
FT 2. SV40, polyA/tk gene
FT 3. RSV, LTR
FT 4. pBR322 4361bp
FT 5. E. coli 1142bp, hyg gene
FT -> pEBVHisB 10307bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 0..0
FT /note="MCS"
FT misc_binding 1..1
FT /note="SIT enterokinase (EK)"
FT repeat_unit 0..0
FT /note="RPT histidine (6)"
FT misc_feature 0..0
FT /note="ATG"
FT promoter complement(0..0)
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli hygromycin phosphotransferase gene;
FT hygromycin resistance gene (hyg)"
FT promoter complement(0..0)
FT /note="PRO SV40 thymidine kinase (TK) gene"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
XX
SQ Sequence 10307 BP; 2526 A; 2586 C; 2932 G; 2263 T; 0 other;
tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
gatctcgagc tcggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat ggaggtgcac
accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact taaatacaat
tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg tctgttgcct
tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagtgtat
tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga
ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta gtcccctcag
atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcagca
gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat aagctgtcaa
acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt tgggggtcgg
gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc
ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc agaccccacg
caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccct
attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg ggtcgctgca
gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg acctgggacc
gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc tgggcggggt
ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa
acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag gccaggaacc
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat
gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc
gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg
ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg
ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg
aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg
ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc
aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg
agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg
aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg
gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag
tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag
gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc
agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg
agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg
gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga
aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg
ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg
aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat
ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat
ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt
tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg
agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga
cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg
taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga
ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca
ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc
tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
agcctatgct acccagatat agattaggat agcatatgct acccagatat agattaggat
agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat taggatagca
tatactaccc taatctctat taggatagca tatgctaccc ggatacagat taggatagca
tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta ccagcgaaga
aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//