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ID   PEBVHISC   preliminary; circular DNA; SYN; 10315 BP.
XX
AC   IG1065; K01383;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pEBVHisC - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pcDV1/S2 from pcDV1 & linker
RC   pRSVCAT/H2 from pRSVcat & linker
RC   [pBT from BT gene]
RC   pRSV/BT from pRSVCAT/H2 & pBT
RC   pRSVPA1 from pRSV/BT & pcDV1/S2
RC   pRSVPA2 from pRSVPA1 & linker
RC   p220.2 from p201
RC   p220.2/B- from p220.2
RC   pREP2 from pRSVPA2 & p220.2/B-
RC   pREP3 from pREP2
RC   p220.2/XS from p220.2/B-
RC   pHS1PA4 from pRSVPA2
RC   pMEP4 from p220.2/XS & pHS1PA4
RC   pRSVCAT-B2 from pRSVcat & linker
RC   pRSVCAT-B2K from pRSVCAT-B2 & linker
RC   pREP4 from pRSVCAT-B2K & pMEP4
RC   pREP5 from pREP4 & oligo
RA   Groger R.K., Morrow D.M., Tykocinski M.L.;
RT   "Directional antisense and sense cDNA cloning using Epstein-Barr
RT   virus episomal expression vectors";
RL   Gene 81:285-294(1989).
XX
CC   NM (pEBVHisC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)
CC   BR (pEBVHisA)(pEBVHisB)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EBV, EBNA-1
FT                   2. SV40, polyA/tk gene
FT                   3. RSV, LTR
FT                   4. pBR322 4361bp
FT                   5. E. coli 1142bp, hyg gene
FT                   -> pEBVHisC 10315bp"
FT   misc_binding    1..1
FT                   /note="SIT XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   misc_binding    1..1
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
XX
SQ   Sequence 10315 BP; 2528 A; 2588 C; 2934 G; 2265 T; 0 other;
     tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
     acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
     tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
     gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt
     gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg
     aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta
     aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc
     tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
     tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
     aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
     tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt
     cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa
     gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg
     ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg
     gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag
     accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
     ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct
     gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
     gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
     accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
     cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
     tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
     atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
     gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
     ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
     agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
     gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
     cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
     aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
     gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
     ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
     acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
     gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
     aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg
     tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
     ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
     ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
     gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg
     ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc
     caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     attctcatgt ttgacagctt atcatcgcga taagctgatc ctcacaggcc gcacccagct
     tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt
     cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc
     ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa
     ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg
     acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact
     ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc
     atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca
     tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga
     cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca
     ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt
     ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca
     ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
     gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
     ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
     ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
     gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
     ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca
     ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca
     ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
     gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
     ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
     ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
     ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga
     ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga
     gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg
     cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca
     ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa
     ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat
     gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa
     aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac
     attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa
     ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta
     aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc
     tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt
     tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag
     gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt
     gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag
     ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag
     gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc
     actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg
     tcttgtcacc attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt
     cactcagctc cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt
     gagcaatcag acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag
     caaccagcat gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
     catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
     atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
     attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
     attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
     attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
     ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
     ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
     ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
     ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
     accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
     cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
     cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
     accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
     aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
     gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
     gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
     tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
     ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
     agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
     ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
     tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
     acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
     ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
     ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
     tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
     taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
     attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
     ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
     atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
     gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
     taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
     tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
     tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
     aaccaactga agggattaca tgcactgccc cgaatacaaa acaaaagcgc tcctcgtacc
     agcgaagaag gggcagagat gccgtagtca ggtttagttc gtccggcggc ggggc
//