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ID   PEMBL18M   preliminary; circular DNA; SYN; 3960 BP.
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AC   IG0311;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phasmid vector pEMBL18- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pEMBL series from pUC18 & pUC19 & pD4, f1 ori
RA   Dente L., Sollazzo M., Baldari C., Cesareni G., Cortese R.;
RT   "The pEMBL family of single-stranded vectors";
RL   DNA Cloning 1:101-106(1985).
RL   Glover D.M., ed., IRL Press, Oxford.
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RN   [2]
RC   pEMBL8+, pEMBL8- from pD4 & pUC8
RC   pEMBL9+, pEMBL9- from pD4 & pUC9
RC   pEMBL18+ from pEMBL8+ & M13mp18
RC   pEMBL18- from pEMBL8- & M13mp18
RC   pEMBL19+ from pEMBL8+ & M13mp19
RC   pEMBL19- from pEMBL8- & M13mp19
RC   pEMBL130+ from pEMBL8+ & M13tg130
RC   pEMBL130- from pEMBL8- & M13tg130
RC   pEMBL131+ from pEMBL8+ & M13tg131
RC   pEMBL131- from pEMBL8- & M13tg131
RA   Dente L., Cortese R.;
RT   "pEMBL: a new family of single-stranded plasmids for sequencing DNA";
RL   Meth. Enzymol. 155:111-119(1987).
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CC   pEMBL vectors are smaller and stabler with large inserts than M13.
CC   The f1 replication proceeds opposite to AMP transcription.
CC   The plus and minus indicate the f1 IG insert direction.
CC   old Boehringer vector.
CC   NM (pEMBL18-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (-)
CC   TY (phasmid)
CC   SP ()
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)
CC   CP ()
CC   FN (packaging of plus single strand DNA in F' strains)
CC   FN (sequencing dideoxy)(in vitro mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC18)(pEMBL8-)
CC   BR (pEMBL18+)(pEMBL19+)(pEMBL19-)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL8- remove PvuII-PvuII 301bp 53..354,
FT                   \ MCS/lacZ alpha 3638bp
FT                   2. M13mp18 PvuII-PvuII 322bp 6055..6377,MCS/lacZ alpha
FT                   -> pEMBL18- 3960bp"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   BspMI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 (-) (1260 bp),
FT                   makes - coding strand,
FT                   EcoRI fragment from pD4 inserted at NarI site"
FT   misc_binding    818..818
FT                   /note="SIT unique ClaI"
FT   misc_binding    1142..1142
FT                   /note="SIT unique DraIII"
FT   misc_binding    1245..1245
FT                   /note="SIT unique NaeI"
FT   misc_binding    1964..1964
FT                   /note="SIT unique DraII"
FT   misc_binding    2025..2025
FT                   /note="SIT unique AatII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2344..2344
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2463..2463
FT                   /note="SIT unique ScaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    3832..3832
FT                   /note="SIT unique AflIII"
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SQ   Sequence 3960 BP; 1083 A; 943 C; 965 G; 967 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcgagctcg
     gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt
     ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     ttgcgcagcc tgaatggcga atggaanttc caacatccaa taaatcatac aggcaaggca
     aagaattagc aaaattaagc aataaagcct cagagcataa agctaaatcg gttgtaccaa
     aaacattatg accctgtaat acttttgcgg gagaagcctt tatttcaacg caaggataaa
     aatttttaga accctcatat attttaaatg caatgcctga gtaatgtgta ggtaaagatt
     caaacgggtg agaaaggccg gagacagtca aatcaccatc aatatgatat tcaaccgttc
     tagctgataa attcatgccg gagagggtag ctatttttga gaggtctcta caaaggctat
     caggtcattg cctgagagtc tggagcaaac aagagaatcg atgaacggta atcgtaaaac
     tagcatgtca atcatatgta ccccggttga taatcagaaa agccccaaaa acaggaagat
     tgtataagca aatatttaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtactatgg
     ttgctttgac gagcacgtat aacgtgcttt cctcgttaga atcagagcgg gagctaaaca
     ggaggccgat taaagggatt ttagacagga acggtacgcc agaatcctga gaagtgtttt
     tataatcagt gaggccaccg agtaaaagag tctgtccatc acgcaaatta accgttgtcg
     caatacttct ttgattagta ataacatcac ttgcctgagt agaagaactc aaactatcgg
     ccttgctggt aatatccaga acaatattac cgccagccat tgcaacagga aaaacgctca
     tggaaatacc tacattttga cgctcaatcg tctggaantt cctgatgcgg tattttctcc
     ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
//