back Return to this vector's summary.
ID   PEMBL18NOT preliminary; circular DNA; SYN; 3972 BP.
XX
AC   M18133; ATCC37573;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pEMBL18-Not (Sma-) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRK419lac from pRK419 & pAD3
RC   pcos4EMBLlac from pcos4EMBL & pAD3
RC   pcos1EMBL from pcos4EMBLlac & pRK419lac
RC   cos793 from pcos1EMBL
RC   cos798 from cos793
RC   cosU800 from pcos1EMBL & pUC9
RC   cos2801 from cos798 & cosU800
RC   pcos2EMBL from cos2801
RC   cos786 from pcos1EMBL & pGD3
RC   cos790 from cos786
RC   cos806 from cos790
RC   cos807 from cos806
RC   cos808 from cos807
RC   cos809 from cos808 & pcos2EMBL
RC   cos810K from cos809
RC   pcos3EMBL from cos810K
RC   cos851 from pcos3EMBL & pUC2
RC   cos852 from cos851
RC   pUC7deltaSal from pUC7
RC   cos854 from cos852 & pUC7deltaSal
RC   pcos5EMBL from cos854
RC   pM1380 from pir41 gene
RC   cos860 from pcos5EMBL & pM1380, pir41 gene
RC   pcos6EMBL from cos860
RC   pEMBL18-Not from R6K
RA   Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT   "A family of cosmid vectors with the multi-copy R6K replication
RT   origin";
RL   Gene 57:229-237(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   General purpose vector modified to include a NotI site to facilitate
CC   library construction and chromosome walking.
CC   SmaI site in polylinker of pEMBL18 changed to a NotI site.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--3.9; NotI--4.0; EcoRI--3.9. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBL18-Not Sma-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL18+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL18+ XbaI 3960bp 256..256
FT                   fill in
FT                   SmaI 3960bp 247..247
FT                   NotI linker 8bp gcggccgc
FT                   -> pEMBL18-Not 3968bp"
FT   -               1..246
FT                   /note="pEMBL18+ 1..246 246bp
FT                   SmaI = CCC^GGG
FT                   NotI =     GC^GGCCGC"
FT   -               247..254
FT                   /note="gcggccgc 8bp
FT                   NotI =  GC^GGCCGC
FT                   SmaI =        CCC^GGG"
FT   -               255..267
FT                   /note="pEMBL18+ 247..259 13bp
FT                   XbaI = T^CTAG A
FT                   XbaI =      T^CTAGA"
FT   -               268..3972
FT                   /note="pEMBL18+ 256..3960 3705bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-NotI-BamHI-SalI-PstI-
FT                   SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-NotI-BamHI-SalI-
FT                   PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3972 BP; 961 A; 977 C; 941 G; 1091 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcgagctcg
     gtacccgcgg ccgcggggat cctctagcta gagtcgacct gcaggcatgc aagcttggca
     ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     ccttcccaac agttgcgcag cctgaatggc gaatggaant tccagacgat tgagcgtcaa
     aatgtaggta tttccatgag cgtttttcct gttgcaatgg ctggcggtaa tattgttctg
     gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact
     aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg gacagactct tttactcggt
     ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct gtctaaaatc
     cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag cacgttatac
     gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta
     aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtaca
     tatgattgac atgctagttt tacgattacc gttcatcgat tctcttgttt gctccagact
     ctcaggcaat gacctgatag cctttgtaga gacctctcaa aaatagctac cctctccggc
     atgaatttat cagctagaac ggttgaatat catattgatg gtgatttgac tgtctccggc
     ctttctcacc cgtttgaatc tttacctaca cattactcag gcattgcatt taaaatatat
     gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc aaaagtatta
     cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc tttattgctt
     aattttgcta attctttgcc ttgcctgtat gatttattgg atgttggaan ttcctgatgc
     ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     aagcggaaga gc
//