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ID PEMBL18NOT preliminary; circular DNA; SYN; 3972 BP.
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AC M18133; ATCC37573;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pEMBL18-Not (Sma-) - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pRK419lac from pRK419 & pAD3
RC pcos4EMBLlac from pcos4EMBL & pAD3
RC pcos1EMBL from pcos4EMBLlac & pRK419lac
RC cos793 from pcos1EMBL
RC cos798 from cos793
RC cosU800 from pcos1EMBL & pUC9
RC cos2801 from cos798 & cosU800
RC pcos2EMBL from cos2801
RC cos786 from pcos1EMBL & pGD3
RC cos790 from cos786
RC cos806 from cos790
RC cos807 from cos806
RC cos808 from cos807
RC cos809 from cos808 & pcos2EMBL
RC cos810K from cos809
RC pcos3EMBL from cos810K
RC cos851 from pcos3EMBL & pUC2
RC cos852 from cos851
RC pUC7deltaSal from pUC7
RC cos854 from cos852 & pUC7deltaSal
RC pcos5EMBL from cos854
RC pM1380 from pir41 gene
RC cos860 from pcos5EMBL & pM1380, pir41 gene
RC pcos6EMBL from cos860
RC pEMBL18-Not from R6K
RA Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT "A family of cosmid vectors with the multi-copy R6K replication
RT origin";
RL Gene 57:229-237(1987).
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CC Created by Moore, July 1995, under contract with NCBI.
CC General purpose vector modified to include a NotI site to facilitate
CC library construction and chromosome walking.
CC SmaI site in polylinker of pEMBL18 changed to a NotI site.
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--3.9; NotI--4.0; EcoRI--3.9. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NM (pEMBL18-Not Sma-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (ATCC)
CC HO (E.coli JM101)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pEMBL18+)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pEMBL18+ XbaI 3960bp 256..256
FT fill in
FT SmaI 3960bp 247..247
FT NotI linker 8bp gcggccgc
FT -> pEMBL18-Not 3968bp"
FT - 1..246
FT /note="pEMBL18+ 1..246 246bp
FT SmaI = CCC^GGG
FT NotI = GC^GGCCGC"
FT - 247..254
FT /note="gcggccgc 8bp
FT NotI = GC^GGCCGC
FT SmaI = CCC^GGG"
FT - 255..267
FT /note="pEMBL18+ 247..259 13bp
FT XbaI = T^CTAG A
FT XbaI = T^CTAGA"
FT - 268..3972
FT /note="pEMBL18+ 256..3960 3705bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-SacI-KpnI-NotI-BamHI-SalI-PstI-
FT SphI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-SacI-KpnI-NotI-BamHI-SalI-
FT PstI-SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 3972 BP; 961 A; 977 C; 941 G; 1091 T; 2 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcgagctcg
gtacccgcgg ccgcggggat cctctagcta gagtcgacct gcaggcatgc aagcttggca
ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
ccttcccaac agttgcgcag cctgaatggc gaatggaant tccagacgat tgagcgtcaa
aatgtaggta tttccatgag cgtttttcct gttgcaatgg ctggcggtaa tattgttctg
gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact
aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg gacagactct tttactcggt
ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct gtctaaaatc
cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag cacgttatac
gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta
aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtaca
tatgattgac atgctagttt tacgattacc gttcatcgat tctcttgttt gctccagact
ctcaggcaat gacctgatag cctttgtaga gacctctcaa aaatagctac cctctccggc
atgaatttat cagctagaac ggttgaatat catattgatg gtgatttgac tgtctccggc
ctttctcacc cgtttgaatc tttacctaca cattactcag gcattgcatt taaaatatat
gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc aaaagtatta
cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc tttattgctt
aattttgcta attctttgcc ttgcctgtat gatttattgg atgttggaan ttcctgatgc
ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
aagcggaaga gc
//