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ID PEMBL18P preliminary; circular DNA; SYN; 3960 BP.
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AC IG0310;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phasmid vector pEMBL18+ - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pEMBL series from pUC18 & pUC19 & pD4, f1 ori
RA Dente L., Sollazzo M., Baldari C., Cesareni G., Cortese R.;
RT "The pEMBL family of single-stranded vectors";
RL DNA Cloning 1:101-106(1985).
RL Glover D.M., ed., IRL Press, Oxford.
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RN [2]
RC pEMBL8+, pEMBL8- from pD4 & pUC8
RC pEMBL9+, pEMBL9- from pD4 & pUC9
RC pEMBL18+ from pEMBL8+ & M13mp18
RC pEMBL18- from pEMBL8- & M13mp18
RC pEMBL19+ from pEMBL8+ & M13mp19
RC pEMBL19- from pEMBL8- & M13mp19
RC pEMBL130+ from pEMBL8+ & M13tg130
RC pEMBL130- from pEMBL8- & M13tg130
RC pEMBL131+ from pEMBL8+ & M13tg131
RC pEMBL131- from pEMBL8- & M13tg131
RA Dente L., Cortese R.;
RT "pEMBL: a new family of single-stranded plasmids for sequencing DNA";
RL Meth. Enzymol. 155:111-119(1987).
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CC pEMBL vectors are smaller and stabler with large inserts than M13.
CC The f1 replication proceeds opposite to AMP transcription.
CC The plus and minus indicate the f1 IG insert direction.
CC old Boehringer vector.
CC NM (pEMBL18+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (+)
CC TY (phasmid)
CC SP ()
CC HO (E.coli JM83)(E.coli JM105)(E.coli JM109)
CC CP ()
CC FN (packaging of plus single strand DNA in F' strains)
CC FN (sequencing dideoxy)(in vitro mutagenesis)
CC SE (color blue/white)
CC PA (pUC18)(pEMBL8+)
CC BR (pEMBL18-)(pEMBL19+)(pEMBL19-)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pEMBL8+ remove PvuII-PvuII 301bp 53..354,
FT \ MCS/lacZ alpha 3638bp
FT 2. M13mp18 PvuII-PvuII 322bp 6055..6377,MCS/lacZ alpha
FT -> pEMBL18+ 3960bp"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_binding 0..0
FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT BspMI-PstI-SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1 (+) (1260 bp),
FT makes + non-coding strand,
FT EcoRI fragment from pD4 inserted at NarI site"
FT misc_binding 818..818
FT /note="SIT unique ClaI"
FT misc_binding 1142..1142
FT /note="SIT unique DraIII"
FT misc_binding 1245..1245
FT /note="SIT unique NaeI"
FT misc_binding 1964..1964
FT /note="SIT unique DraII"
FT misc_binding 2025..2025
FT /note="SIT unique AatII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 2344..2344
FT /note="SIT unique Asp700I"
FT misc_binding 2463..2463
FT /note="SIT unique ScaI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 3832..3832
FT /note="SIT unique AflIII"
XX
SQ Sequence 3960 BP; 960 A; 972 C; 936 G; 1090 T; 2 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcgagctcg
gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt
ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
ttgcgcagcc tgaatggcga atggaanttc cagacgattg agcgtcaaaa tgtaggtatt
tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc
ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat
gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga
cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat gaatttatca
gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct ttctcacccg
tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga gggttctaaa
aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca gggtcataat
gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa ttttgctaat
tctttgcctt gcctgtatga tttattggat gttggaantt cctgatgcgg tattttctcc
ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
//