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ID   PEMBL8CAT  preliminary; circular DNA; SYN; 4712 BP.
XX
AC   IG9869;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pEMBL8-CAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pEMBL8-CAT from pEMBL8 & cat gene
RC   pUMSSB1 from mouse c-mos gene
RC   pE8UCAT from pEMBL8-CAT & pUMSSB1
RA   Piaggio G.;
RT   ;
RL   Focus 12:83-84(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pEMBL8-CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL8+)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL8+ TaqI 3939bp 3714..3714, ori/amp
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pEMBL8-CAT 4712bp"
FT   -               1..3713
FT                   /note="pEMBL8+ 1..3713 3713bp
FT                   TaqI = T^CGA"
FT   -               3714..4486
FT                   /note="Tn9 215..987 773bp
FT                   TaqI = T^CGA"
FT   -               4487..4712
FT                   /note="pEMBL8+ 3714..3939 226bp"
FT   misc_binding    0..0
FT                   /note="MCS XmaI-SmaI-BamHI-HincII-AccI-SalI-PstI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmaI-SmaI-BamHI-HincII-AccI-SalI-
FT                   PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4712 BP; 1166 A; 1130 C; 1109 G; 1305 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcccgggga
     tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt
     gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct
     tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg
     cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat
     tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
     gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
     tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg
     gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt
     catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac
     ctctcaaaaa tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat
     attgatggtg atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat
     tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa
     ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta
     gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat
     ttattggatg ttggaanttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgagatt
     ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat ttttgtgatg
     ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     cagcgagtca gtgagcgagg aagcggaaga gc
//