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ID PEMBL9P preliminary; circular DNA; SYN; 3939 BP.
XX
AC IG1380; X04995; ATCC37395;
XX
DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phasmid vector pEMBL9+ - complete.
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KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pEMBL8, pEMBL8+, pEMBL8- from pUC8 & pD4
RC plasmid from pEMBL8
RC lambda G7 from plasmid & lambda NM616
RC lambda G43 from lambda G7 & pUC9
RC pEMBL9, pEMBL9+, pEMBL9- from plasmid & lambda G43
RA Dente L., Cesareni G., Cortese R.;
RT "pEMBL: a new family of single stranded plasmids";
RL Nucleic Acids Res. 11:1645-1654(1983).
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RN [2]
RP 1-3939
RC pEMBL9+
RA Pfeiffer F.;
RT ;
RL Submitted (02-JUN-1987) on paper to the EMBL Data Library by:
RL Pfeiffer F., MPI Biochemie, Martinsried, Germany.
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RN [3]
RC pEMBL8, pEMBL8+, pEMBL8- from pD4 & pUC8
RC pEMBL9, pEMBL9+, pEMBL9- from pD4 & pUC9
RC pEMBL18+ from pEMBL8+ & M13mp18
RC pEMBL18- from pEMBL8- & M13mp18
RC pEMBL19+ from pEMBL8+ & M13mp19
RC pEMBL19- from pEMBL8- & M13mp19
RC pEMBL130+ from pEMBL8+ & M13tg130
RC pEMBL130- from pEMBL8- & M13tg130
RC pEMBL131+ from pEMBL8+ & M13tg131
RC pEMBL131- from pEMBL8- & M13tg131
RA Dente L., Cortese R.;
RT "pEMBL: a new family of single-stranded plasmids for sequencing DNA";
RL Meth. Enzymol. 155:111-119(1987).
XX
CC According to the cloning strategy, the phage f1 origin should
CC be flanked by regenerated EcoRI sites, but these are not found
CC experimentally.
CC The given sequence must be incorrect around this position.
CC The base n was introduced to destroy the EcoRI site (GAAnTTC).
CC ssDNA-producing plasmid with polylinker in lacZ'.
CC ATCC depositor: Cesareni G.
CC Medium is 1227 LB plus ampicillin.
CC NM (pEMBL9+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phasmid)
CC SP (ATCC)
CC HO (E.coli 71/18)(E.coli)(E.coli JM series)
CC CP ()
CC FN (transcription)
CC SE ()
CC PA (pUC9)(M13mp9)
CC BR (pEMBL9-)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC9 NarI 2665bp 426..426, between amp & lac
FT 2. pD4 EcoRI-? 1260bp 4292..5552, f1 ori
FT -> pEMBL9+ 3939bp"
FT misc_feature 1..423
FT /note="from pUC9 (bases 1..423)"
FT misc_feature 228..263
FT /note="MCS HindIII-PstI-AvaI-SalI-BamHI-SmaI-EcoRI,
FT from M13mp9/pUC9 polylinker (bases 1..36)"
FT misc_feature 423..429
FT /note="destroyed EcoRI site (GAAnTTC) (bases 1..7)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_feature 430..1692
FT /note="from bacteriophage f1,
FT bases complement(5147..6407)"
FT misc_feature 1375..1378
FT /note="AGAG in pEMBL9+; AG in bacteriophage f1"
FT misc_feature 1694..1700
FT /note="destroyed EcoRI site (GAAnTTC) (bases 1..7)"
FT misc_feature 1700..3939
FT /note="from pUC9 (bases 426..2665)"
FT misc_feature 2205..2993
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp) (bases 1..789)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 0..0
FT /note="SIT ClaI"
FT misc_binding 0..0
FT /note="SIT EcoB"
FT misc_binding 0..0
FT /note="SIT NaeI"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
XX
SQ Sequence 3939 BP; 955 A; 966 C; 931 G; 1085 T; 2 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
tggaanttcc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt
gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct
tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg
cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat
tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg
gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt
catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac
ctctcaaaaa tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat
attgatggtg atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat
tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa
ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta
gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat
ttattggatg ttggaanttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
ccgagcgcag cgagtcagtg agcgaggaag cggaagagc
//