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ID   PEMBLYE23  preliminary; circular DNA; SYN; 7282 BP.
XX
AC   X04996; X04995; ATCC37400;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pEMBLYe23 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYe23)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 HindIII 7769bp 2273..2273
FT                   Klenow
FT                   -> YEP24-H 7769bp
FT                   1. pEMBL9+ ClaI 3939bp 1324..1324, near f1 ori
FT                   Klenow
FT                   2. YEP24-H HindIII-HindIII 3333bp 106..3439,
FT                   \ yeast 2 micron/URA3
FT                   Klenow:Klenow
FT                   -> pEMBLYe23 7280bp"
FT   -               1..1325
FT                   /note="pEMBL9+ 1..1325 1325bp
FT                   ClaI = AT^CG AT
FT                   HindIII =  A^AGCTT"
FT   -               1326..3496
FT                   /note="YEp24 106..2276 2171bp
FT                   HindIII =  A^AGCT T
FT                   ClaI =         AT^CGAT"
FT   -               3497..4666
FT                   /note="YEp24 2273..3442 1170bp
FT                   HindIII =  A^AGCT T
FT                   HindIII =       A^AGCTT"
FT   -               4667..7282
FT                   /note="pEMBL9+ 1324..3939 2616bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-HindIII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 7282 BP; 2038 A; 1576 C; 1633 G; 2033 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
     ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt
     gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct
     tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg
     cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat
     tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
     gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
     tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg
     gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt
     catcgagctt tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa
     tctcacattg gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg
     actaatgttg tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat
     actcatcaga taacagcaat acctgatcac tacttcgcac tagtttctcg gtactatgca
     tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag
     tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg
     aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata
     taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac
     aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt
     gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat
     tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc
     actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc
     gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta
     tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt
     ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat
     agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca
     aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt
     ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc
     ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc
     tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta
     cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat
     ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga
     agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat
     gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa
     aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta
     aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct
     acaaaatgaa gcacagatgc ttcgttaaca aagatatgct attgaagtgc aagatggaaa
     cgcagaaaat gaaccgggga tgcgacgtgc aagattacct atgcaataga tgcaatagtt
     tctccaggaa ccgaaataca tacattgtct tccgtaaagc gctagactat atattattat
     acaggttcaa atatactatc tgtttcaggg aaaactccca ggttcggatg ttcaaaattc
     aatgatgggt aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat attaggctgt
     atctcctcaa agcgtattcg aatatcattg agaagctgca gcgtcacatc ggataataat
     gatggcagcc attgtagaag tgccttttgc atttctagtc tctttctcgg tctagctagt
     tttactacat cgcgaagata gaatcttaga tcacactgcc tttgctgagc tggatcaata
     gagtaacaaa agagtggtaa ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg
     tacagtagac ggagtatact agtatagtct atagtccgtg gaattctcat gtttgacagc
     ttatcatcga taagctagct tttcaattca attcatcatt ttttttttat tctttttttt
     gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     actcacaaat tagagcttca atttaattat atcagttatt acccgggaat ctcggtcgta
     atgattttta taatgacgaa aaaaaaaaaa ttggaaagaa aaagctcgat tctcttgttt
     gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa aaatagctac
     cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg gtgatttgac
     tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag gcattgcatt
     taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc
     aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc
     tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg atgttggaan
     ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     gtgagcgagg aagcggaaga gc
//