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ID   PEMBLYE24  preliminary; circular DNA; SYN; 7282 BP.
XX
AC   X04996; X04995; ATCC37401;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pEMBLYe24 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYe24)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 HindIII 7769bp 2273..2273
FT                   Klenow
FT                   -> YEP24-H 7769bp
FT                   1. pEMBL9- ClaI 3939bp 798..798, near f1 ori
FT                   Klenow
FT                   2. YE24-H HindIII-HindIII 3333bp 106..3439,
FT                   \ yeast 2 micron/URA3
FT                   Klenow:Klenow
FT                   -> pEMBLYe24 7380bp"
FT   -               1..799
FT                   /note="pEMBL9- 1..799 799bp
FT                   ClaI = AT^CG AT
FT                   HindIII =  A^AGCTT"
FT   -               800..2970
FT                   /note="YEp24 106..2276 2171bp
FT                   HindIII =  A^AGCT T
FT                   ClaI =         AT^CGAT"
FT   -               2971..4140
FT                   /note="YEp24 2273..3442 1170bp
FT                   HindIII =  A^AGCT T
FT                   HindIII =       A^AGCTT"
FT   -               4141..7282
FT                   /note="pEMBL9- 798..3939 3142bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-AvaI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI-NcoI-EcoRV-BamHI-HindIII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 7282 BP; 2161 A; 1547 C; 1662 G; 1910 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
     ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc aacatccaat aaatcataca ggcaaggcaa agaattagca aaattaagca
     ataaagcctc agagcataaa gctaaatcgg ttgtaccaaa aacattatga ccctgtaata
     cttttgcggg agaagccttt atttcaacgc aaggataaaa atttttagaa ccctcatata
     ttttaaatgc aatgcctgag taatgtgtag gtaaagattc aaacgggtga gaaaggccgg
     agacagtcaa atcaccatca atatgatatt caaccgttct agctgataaa ttcatgccgg
     agagggtagc tatttttgag aggtctctac aaaggctatc aggtcattgc ctgagagtct
     ggagcaaaca agagaatcga gctttgaaga aaaatgcgcc ttattcaatc tttgctataa
     aaaatggccc aaaatctcac attggaagac atttgatgac ctcatttctt tcaatgaagg
     gcctaacgga gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg
     ccaggacaac gtatactcat cagataacag caatacctga tcactacttc gcactagttt
     ctcggtacta tgcatatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta
     atccaattga ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac
     gataccccgc atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca
     taaatagacg catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat
     gtatatatat atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt
     gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat
     tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag
     cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa
     atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct
     gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac
     tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag
     taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt
     agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc
     aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg
     gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg
     aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa
     ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg
     agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg
     tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc
     tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat
     ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc
     atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
     tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa
     gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa
     tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga
     ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg
     gatgttcaaa attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc
     ggatattagg ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcagcgtca
     catcggataa taatgatggc agccattgta gaagtgcctt ttgcatttct agtctctttc
     tcggtctagc tagttttact acatcgcgaa gatagaatct tagatcacac tgcctttgct
     gagctggatc aatagagtaa caaaagagtg gtaaggcctc gttaaaggac aaggacctga
     gcggaagtgt atcgtacagt agacggagta tactagtata gtctatagtc cgtggaattc
     tcatgtttga cagcttatca tcgataagct agcttttcaa ttcaattcat catttttttt
     ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa tctccgaaca
     gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt
     tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag
     gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag
     tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc
     attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat
     ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa
     gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc
     tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg
     ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca
     ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg
     caagggctcc ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga
     caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta
     cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca
     acagtataga accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag
     aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg
     ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa
     tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccgg
     gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa aaaattggaa agaaaaagct
     cgatgaacgg taatcgtaaa actagcatgt caatcatatg taccccggtt gataatcaga
     aaagccccaa aaacaggaag attgtataag caaatattta aattgtaaac gttaatattt
     tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     cgctacaggg cgcgtactat ggttgctttg acgagcacgt ataacgtgct ttcctcgtta
     gaatcagagc gggagctaaa caggaggccg attaaaggga ttttagacag gaacggtacg
     ccagaatcct gagaagtgtt tttataatca gtgaggccac cgagtaaaag agtctgtcca
     tcacgcaaat taaccgttgt cgcaatactt ctttgattag taataacatc acttgcctga
     gtagaagaac tcaaactatc ggccttgctg gtaatatcca gaacaatatt accgccagcc
     attgcaacag gaaaaacgct catggaaata cctacatttt gacgctcaat cgtctggaan
     ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     gtgagcgagg aagcggaaga gc
//