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ID   PEMBLYI22  preliminary; circular DNA; SYN; 5111 BP.
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AC   X04996; X04995; ATCC37399;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pEMBLYi22 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYi22)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL9- ClaI 3939bp 798..798, near f1 ori
FT                   Klenow
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   Klenow:Klenow
FT                   -> pEMBLYi22 5105bp"
FT   -               1..799
FT                   /note="pEMBL9- 1..799 799bp
FT                   ClaI = AT^CG AT
FT                   HindIII =  A^AGCTT"
FT   -               800..1969
FT                   /note="YEp24 2273..3442 1170bp
FT                   HindIII =  A^AGCT T
FT                   ClaI =         AT^CGAT"
FT   -               1970..5111
FT                   /note="pEMBL9- 798..3939 3142bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-AvaI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-SalI-HindIII-ApaI-
FT                   NcoI-EcoRV"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 5111 BP; 1461 A; 1125 C; 1232 G; 1291 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
     ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc aacatccaat aaatcataca ggcaaggcaa agaattagca aaattaagca
     ataaagcctc agagcataaa gctaaatcgg ttgtaccaaa aacattatga ccctgtaata
     cttttgcggg agaagccttt atttcaacgc aaggataaaa atttttagaa ccctcatata
     ttttaaatgc aatgcctgag taatgtgtag gtaaagattc aaacgggtga gaaaggccgg
     agacagtcaa atcaccatca atatgatatt caaccgttct agctgataaa ttcatgccgg
     agagggtagc tatttttgag aggtctctac aaaggctatc aggtcattgc ctgagagtct
     ggagcaaaca agagaatcga gcttttcaat tcaattcatc attttttttt tattcttttt
     tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac
     gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat
     gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat
     aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
     ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
     gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
     aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
     cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
     atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
     cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
     aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
     tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
     ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
     ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
     ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
     caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
     atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac
     taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg aatctcggtc
     gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaagctc gatgaacggt
     aatcgtaaaa ctagcatgtc aatcatatgt accccggttg ataatcagaa aagccccaaa
     aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc
     gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     gcgtactatg gttgctttga cgagcacgta taacgtgctt tcctcgttag aatcagagcg
     ggagctaaac aggaggccga ttaaagggat tttagacagg aacggtacgc cagaatcctg
     agaagtgttt ttataatcag tgaggccacc gagtaaaaga gtctgtccat cacgcaaatt
     aaccgttgtc gcaatacttc tttgattagt aataacatca cttgcctgag tagaagaact
     caaactatcg gccttgctgg taatatccag aacaatatta ccgccagcca ttgcaacagg
     aaaaacgctc atggaaatac ctacattttg acgctcaatc gtctggaant tcctgatgcg
     gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     agcggaagag c
//