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ID PEMBLYI22 preliminary; circular DNA; SYN; 5111 BP.
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AC X04996; X04995; ATCC37399;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli phagemid vector pEMBLYi22 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC YEp24-H from YEp24
RC pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC pEMBLYr25 from pEMBL9 & YRp7
RC pEMBLYi27 from pEMBL9 & pCV13
RC pEMBLYe30 from pEMBL9 & pCV13
RA Baldari C., Cesareni G.;
RT "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT recovery and analysis of yeast DNA sequences";
RL Gene 35:27-32(1985).
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CC Created by Moore, July 1995, under contract with NCBI.
CC ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC in yeast.
CC Medium is 1227 LB plus ampicillin.
CC NM (pEMBLYi22)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (ATCC)
CC HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC HO (E.coli JM series)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pEMBL9+)(YEp24)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pEMBL9- ClaI 3939bp 798..798, near f1 ori
FT Klenow
FT 2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT Klenow:Klenow
FT -> pEMBLYi22 5105bp"
FT - 1..799
FT /note="pEMBL9- 1..799 799bp
FT ClaI = AT^CG AT
FT HindIII = A^AGCTT"
FT - 800..1969
FT /note="YEp24 2273..3442 1170bp
FT HindIII = A^AGCT T
FT ClaI = AT^CGAT"
FT - 1970..5111
FT /note="pEMBL9- 798..3939 3142bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-SmaI-BamHI-SalI-AvaI-PstI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-BamHI-SalI-HindIII-ApaI-
FT NcoI-EcoRV"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
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SQ Sequence 5111 BP; 1461 A; 1125 C; 1232 G; 1291 T; 2 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
tggaanttcc aacatccaat aaatcataca ggcaaggcaa agaattagca aaattaagca
ataaagcctc agagcataaa gctaaatcgg ttgtaccaaa aacattatga ccctgtaata
cttttgcggg agaagccttt atttcaacgc aaggataaaa atttttagaa ccctcatata
ttttaaatgc aatgcctgag taatgtgtag gtaaagattc aaacgggtga gaaaggccgg
agacagtcaa atcaccatca atatgatatt caaccgttct agctgataaa ttcatgccgg
agagggtagc tatttttgag aggtctctac aaaggctatc aggtcattgc ctgagagtct
ggagcaaaca agagaatcga gcttttcaat tcaattcatc attttttttt tattcttttt
tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac
gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat
gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat
aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac
taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg aatctcggtc
gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaagctc gatgaacggt
aatcgtaaaa ctagcatgtc aatcatatgt accccggttg ataatcagaa aagccccaaa
aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc
gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
gcgtactatg gttgctttga cgagcacgta taacgtgctt tcctcgttag aatcagagcg
ggagctaaac aggaggccga ttaaagggat tttagacagg aacggtacgc cagaatcctg
agaagtgttt ttataatcag tgaggccacc gagtaaaaga gtctgtccat cacgcaaatt
aaccgttgtc gcaatacttc tttgattagt aataacatca cttgcctgag tagaagaact
caaactatcg gccttgctgg taatatccag aacaatatta ccgccagcca ttgcaacagg
aaaaacgctc atggaaatac ctacattttg acgctcaatc gtctggaant tcctgatgcg
gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
agcggaagag c
//