back Return to this vector's summary.
ID   PEMBLYI32  preliminary; circular DNA; SYN; 5111 BP.
XX
AC   X04996; X04995; ATCC37406;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pEMBLYi32 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RC   [pEMBLYi32 from pEMBL9]
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYi32)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL9+ ClaI 3939bp 1324..1324, near f1 ori
FT                   Klenow
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   Klenow:Klenow
FT                   -> pEMBLYi32 5105bp"
FT   -               1..1325
FT                   /note="pEMBL9+ 1..1325 1325bp
FT                   ClaI = AT^CG AT
FT                   HindIII =  A^AGCTT"
FT   -               1326..2495
FT                   /note="YEp24 2273..3442 1170bp
FT                   HindIII =  A^AGCT T
FT                   ClaI =         AT^CGAT"
FT   -               2496..5111
FT                   /note="pEMBL9+ 1324..3939 2616bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SstI-KpnI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII-FsaI-XmnI-
FT                   AatII-Tth111I-EcoRV-NcoI-BsmI-ApaI-StuI-NsiI-NaeI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 5111 BP; 1338 A; 1154 C; 1203 G; 1414 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
     ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt
     gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct
     tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg
     cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat
     tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
     gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
     tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg
     gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt
     catcgagctt ttcaattcaa ttcatcattt tttttttatt cttttttttg atttcggttt
     ctttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
     agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
     ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa
     gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
     atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
     ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
     atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
     tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg
     cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
     gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
     cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
     tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
     gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
     gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg
     gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
     gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
     ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
     agagcttcaa tttaattata tcagttatta cccgggaatc tcggtcgtaa tgatttttat
     aatgacgaaa aaaaaaaaat tggaaagaaa aagctcgatt ctcttgtttg ctccagactc
     tcaggcaatg acctgatagc ctttgtagag acctctcaaa aatagctacc ctctccggca
     tgaatttatc agctagaacg gttgaatatc atattgatgg tgatttgact gtctccggcc
     tttctcaccc gtttgaatct ttacctacac attactcagg cattgcattt aaaatatatg
     agggttctaa aaatttttat ccttgcgttg aaataaaggc ttctcccgca aaagtattac
     agggtcataa tgtttttggt acaaccgatt tagctttatg ctctgaggct ttattgctta
     attttgctaa ttctttgcct tgcctgtatg atttattgga tgttggaant tcctgatgcg
     gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     agcggaagag c
//