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ID   PEMBLYR25  preliminary; circular DNA; SYN; 5399 BP.
XX
AC   X04996; X04995; ATCC37402;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pEMBLYr25 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYr25)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(YRp7)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL9+ ClaI 3939bp 1324..1324, near f1 ori
FT                   Klenow
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS/TRP1 gene
FT                   Klenow:Klenow
FT                   -> pEMBLYr25 5384bp"
FT   -               1..1325
FT                   /note="pEMBL9+ 1..1325 1325bp
FT                   ClaI = AT^CG AT
FT                   EcoRI =    G^AATTC"
FT   -               1326..2783
FT                   /note="YRp7 2..1459 1458bp
FT                   EcoRI = G^AATT C
FT                   ClaI =      AT^CGAT"
FT   -               2784..5399
FT                   /note="pEMBL9+ 1324..3939 2616bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-AvaI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV-BglII-MstI-StuI-XbaI-AvaI-
FT                   BamHI-SalI-SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
XX
SQ   Sequence 5399 BP; 1388 A; 1281 C; 1206 G; 1522 T; 2 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacaag cttggctgca
     ggtcgacgga tccccgggaa ttcggcactg gccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggaanttcc agacgattga gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt
     gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct
     tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg
     cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat
     tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct
     gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg
     tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg
     gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt
     catcgaattc ccacatgtta aaatagtgaa ggagcatgtt cggcacacag tggaccgaac
     gtggggtaag tgcactaggg tccggttaaa cggatctcgc attgatgagg caacgctaat
     tatcaacata tagattgtta tctatctgca tgaacacgaa atctttactt gacgacttga
     ggctgatggt gtttatgcaa agaaaccact gtgtttaata tgtgtcactg tttgatatta
     ctgtcagcgt agaagataat agtaaaagcg gttaataagt gtatttgaga taagtgtgat
     aaagttttta cagcgaaaag acgataaata caagaaaatg attacgagga tacggagaga
     ggtatgtaca tgtgtattta tatactaagc tgccggcggt tgtttgcaag accgagaaaa
     ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg aacattctct tcaacaagtt
     tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc gatgtatatt ttcctgtaca
     atcaatcaaa aagccaaatg atttagcatt atctttacat cttgttattt tacagatttt
     atgtttagat cttttatgct tgcttttcaa aaggcctgca ggcaagtgca caaacaatac
     ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt
     ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg
     ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa
     tgtaagcttt cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa
     agttcacctg tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt
     gaagctgcac tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc
     aaaccgagga actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca
     atgccgtaat cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca
     accaagtatt tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc
     cttgcaataa ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag
     tcagcatcgg aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc
     accaatggac cagaactacc tgtgaaatta ataacagaca tactccaagc tgcctttgtg
     tgcttaatca cgtatactca cgtgctcaat agtcaccaat gccctccctc ttggccctct
     ccttttcttt tttcgaccga attcgattct cttgtttgct ccagactctc aggcaatgac
     ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag
     ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt
     ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa
     atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg
     tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt
     ctttgccttg cctgtatgat ttattggatg ttggaanttc ctgatgcggt attttctcct
     tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagc
//