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ID   PET31F1P   preliminary; circular DNA; PRO; 2731 BP.
XX
AC   X62498;
XX
DT   28-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pET31F1P - complete.
XX
KW   cloning vector.
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OS   Escherichia coli
OC   Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC   Facultatively anaerobic rods; Enterobacteriaceae; Escherichia.
XX
RN   [1]
RC   pET31F1P from phage f1 & pET-3c & pSP65
RC   pET81F1P from phage f1 & pET-8c & pSP65
RA   Tanhauser S.M., Jewell D.A., Tu C.K., Silverman D.N., Laipis P.J.;
RT   "A T7 expression vector optimized for site-directed mutagenesis
RT   using oligodeoxyribonucleotide cassettes";
RL   Gene 117:113-117(1992).
XX
RN   [2]
RP   1-2731
RC   pET31F1P
RA   Laipis P.J.;
RT   ;
RL   Submitted (07-OCT-1991) on tape to the EMBL Data Library by:
RL   P.J. Laipis, Univ of Florida College of Medicine,
RL   Dept of Biochemistry & Mol Biology, Box J-245 J H M Health Center,
RL   Gainesville Florida, USA.
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CC   Overlapping sequences:
CC   X04995 (bp 771-1226);
CC   M11662 (bp 631-2617);
CC   NM (pET31F1P)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (F1)(pET3c)(pSP65 from pUC12)
CC   BR (pET81F1P)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP65, MCS
FT                   2. pET3c, ori
FT                   3. phage f1, ori
FT                   -> pET31F1P 2731bp"
FT   rep_origin      1..463
FT                   /note="ORI bacteriophage f1"
FT   misc_feature    466..735
FT                   /note="bacteriophage T7 expression cassette,
FT                   from pET3c"
FT   promoter        485..501
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    565..666
FT                   /note="translation start for inserted gene"
FT   terminator      670..705
FT                   /note="TER bacteriophage T7"
FT   misc_feature    744..2730
FT                   /note="from pUC12 backbone (from pSP65)"
FT   misc_feature    1742..2599
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), precursor"
XX
SQ   Sequence 2731 BP; 677 A; 687 C; 659 G; 708 T; 0 other;
     agatctcacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgagatct cgatcccgcg
     aaattaatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
     taactttaag aaggagatat acatatggct agcatgactg gtggacagca aatgggtcgg
     atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     gaactatatc cggatggcat gccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg g
//