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ID   PET81F1P   preliminary; circular DNA; PRO; 2730 BP.
XX
AC   X62499;
XX
DT   28-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pET81F1P - complete.
XX
KW   cloning vector.
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OS   Escherichia coli
OC   Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC   Facultatively anaerobic rods; Enterobacteriaceae; Escherichia.
XX
RN   [1]
RC   pET31F1P from phage f1 & pET-3c & pSP65
RC   pET81F1P from phage f1 & pET-8c & pSP65
RA   Tanhauser S.M., Jewell D.A., Tu C.K., Silverman D.N., Laipis P.J.;
RT   "A T7 expression vector optimized for site-directed mutagenesis
RT   using oligodeoxyribonucleotide cassettes";
RL   Gene 117:113-117(1992).
XX
RN   [2]
RP   1-2730
RC   pET81F1P
RA   Laipis P.J.;
RT   ;
RL   Submitted (07-OCT-1991) on tape to the EMBL Data Library by:
RL   P.J. Laipis, Univ of Florida College of Medicine,
RL   Dept of Biochemistry & Mol Biology, Box J-245 J H M Health Center,
RL   Gainesville Florida, USA.
XX
CC   Overlapping sequences:
CC   X04995 (bp 771-1226);
CC   M11662 (bp 631-2617);
CC   NM (pET81F1P)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (F1)(pET8c)(pSP65 from pUC12)
CC   BR (pET31F1P)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP65, MCS
FT                   2. pET8c, ori
FT                   3. phage f1, ori
FT                   -> pET81F1P 2730bp"
FT   rep_origin      1..463
FT                   /note="ORI bacteriophage f1"
FT   misc_feature    466..734
FT                   /note="bacteriophage T7 expression cassette,
FT                   from pET8c"
FT   promoter        485..501
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    564..566
FT                   /note="translation start for inserted gene"
FT   terminator      669..704
FT                   /note="TER bacteriophage T7"
FT   misc_feature    743..2729
FT                   /note="from pUC12 backbone (from pSP65)"
FT   misc_feature    1741..2598
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), precursor"
XX
SQ   Sequence 2730 BP; 676 A; 688 C; 659 G; 707 T; 0 other;
     agatctcacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgagatct cgatcccgcg
     aaattaatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
     taactttaag aaggagatat accatggcta gcatgactgg tggacagcaa atgggtcgga
     tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     aactatatcc ggatggcatg ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctgg
//