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ID   PETHISK    preliminary; circular DNA; SYN; 4675 BP.
XX
AC   L20318;
XX
DT   11-JUL-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli vector pET-HisK - complete.
XX
KW   cloning vector; expression vector; fusion protein;
KW   heart muscle kinase;  histidine tagged fusion protein;
KW   phosphorylation; radiolabeling.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4675
RC   pET-His from his
RC   pET-HisK from his & heart muscle kinase gene
RA   Chen B.P., Hai T.;
RT   "Expression vectors for affinity purification and radiolabeling of
RT   proteins using E. coli as host";
RL   Gene 139:73-75(1994).
XX
CC   NCBI gi: 436566
CC   NM (pET-HisK)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pET-His 4636bp
FT                   2. heart muscle 39bp, kinase gene
FT                   -> pET-HisK 4675bp"
FT   CDS             0..0
FT                   /note="GEN E. coli histidine tagged fusion protein"
FT   misc_binding    0..0
FT                   /note="heart muscle kinase (HMK) sites"
XX
SQ   Sequence 4675 BP; 1119 A; 1212 C; 1282 G; 1062 T; 0 other;
     ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
     gatatacata tgcatcacca tcaccatcac ctcgaacgtc gtgcatctgt tggtcgccgt
     gcttccgttc tcgagaccat ggatccggct gctaacaaag cccgaaagga agctgagttg
     gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc
     catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
     aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
     cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
     gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
     ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
//