back Return to this vector's summary.
ID   PEUKC1     preliminary; circular DNA; SYN; 4892 BP.
XX
AC   IG1066;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pEUK-C1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from linker & pPyMT1
RC   pMTSVL1 from pJC119 & plasmid
RC   dl3, dl5, dl13 from pMTSVL1
RA   Templeton D., Eckhart W.;
RT   "N-terminal amino acid sequences of the polyoma middle-size T
RT   antigen are important for protein kinase activity and cell
RT   transformation";
RL   Mol. Cell. Biol. 4:817-821(1984).
XX
RN   [2]
RC   p53.A6.6 from polyoma virus genome
RC   pPyMT1 from p53.A6.6 & pE3.0
RA   Treisman R., Novak U., Favaloro J., Kamen R.;
RT   "Transformation of rat cells by an altered polyoma virus genome
RT   expressing only the middle T protein";
RL   Nature 292:595-600(1981).
XX
RN   [3]
RC   pE3.0 from pAT153 & polyoma virus middle-T protein gene
RA   Treisman R., Cowie A., Favaloro J.M., Jat P., Kamen R.;
RT   "The structures of the spliced mRNAs encoding polyoma virus early
RT   region proteins";
RL   J. Mol. Appl. Genet. 1:83-92(1981).
XX
CC   NM (pEUK-C1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. SV40 500bp, ori/late polyA
FT                   -> pEUK-C1 4892bp"
FT   misc_binding    4359..4359
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO SV40 late genes"
FT   misc_feature    0..0
FT                   /note="SPL SV40 VPI intron"
FT   misc_binding    1502..1531
FT                   /note="MCS XhoI-XbaI-SmaI-SacI-BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 late gene"
FT   misc_binding    2040..2040
FT                   /note="SIT SalI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3607..3607
FT                   /note="SIT PstI"
FT   misc_binding    3733..3733
FT                   /note="SIT PvuI"
XX
SQ   Sequence 4892 BP; 1207 A; 1175 C; 1179 G; 1331 T; 0 other;
     gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
     aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
     ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
     tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
     acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
     tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
     actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
     gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
     tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
     cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
     gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
     tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
     tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
     ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
     aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
     aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
     ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
     aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
     agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
     ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
     tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaccggatc
     gatccctcga gtctagaccc ggggagctcg gatccagaca tgataagata cattgatgag
     tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     ctctacaaat gtggtatggc tgattatgat cctctacgcc ggacgcatcg tggccggcat
     caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     agatgacgac catcagggac agcttcaaag gccagcaaaa ggccaggaac cgtaaaaagg
     ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca tgtttgacag
     cttatcatcg aagcagtagc aatcaaccca cacaagtgga tctttcctgt ataattttct
     attttcatgc ttcatcctca gtaagcacag caagcatatg cagttagcag acattttctt
     tgcacactca ggccattgtt tgcagtacat tgcatcaaca ccaggattta aggaagaagc
     aaatacctca gttgcatccc agaagcctcc aaagtcaggt tgatgagcat attttactcc
     atcttccatt ttcttgtaca gagtattcat tttcttcatt ttttcttcat ctcctccttt
     atcaggatga aactccttgc atttttttaa atatgccttt ctcatcagag gaatattccc
     ccaggcactc ctttcaagac ctagaaggtc cattagctgc aaagattcct ctctgtttaa
     aactttatcc atctttgcaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     actacttctg gaatagctca gaggccgagg cg
//