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ID PEUKC1 preliminary; circular DNA; SYN; 4892 BP.
XX
AC IG1066;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pEUK-C1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from linker & pPyMT1
RC pMTSVL1 from pJC119 & plasmid
RC dl3, dl5, dl13 from pMTSVL1
RA Templeton D., Eckhart W.;
RT "N-terminal amino acid sequences of the polyoma middle-size T
RT antigen are important for protein kinase activity and cell
RT transformation";
RL Mol. Cell. Biol. 4:817-821(1984).
XX
RN [2]
RC p53.A6.6 from polyoma virus genome
RC pPyMT1 from p53.A6.6 & pE3.0
RA Treisman R., Novak U., Favaloro J., Kamen R.;
RT "Transformation of rat cells by an altered polyoma virus genome
RT expressing only the middle T protein";
RL Nature 292:595-600(1981).
XX
RN [3]
RC pE3.0 from pAT153 & polyoma virus middle-T protein gene
RA Treisman R., Cowie A., Favaloro J.M., Jat P., Kamen R.;
RT "The structures of the spliced mRNAs encoding polyoma virus early
RT region proteins";
RL J. Mol. Appl. Genet. 1:83-92(1981).
XX
CC NM (pEUK-C1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (CLONTECH)
CC HO (E.coli)(COS cells)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pBR322)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/amp gene
FT 2. SV40 500bp, ori/late polyA
FT -> pEUK-C1 4892bp"
FT misc_binding 4359..4359
FT /note="SIT EcoRI"
FT promoter 0..0
FT /note="PRO SV40 late genes"
FT misc_feature 0..0
FT /note="SPL SV40 VPI intron"
FT misc_binding 1502..1531
FT /note="MCS XhoI-XbaI-SmaI-SacI-BamHI"
FT polyA_signal 0..0
FT /note="PLA SV40 late gene"
FT misc_binding 2040..2040
FT /note="SIT SalI"
FT rep_origin 0..0
FT /note="ORI SV40"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3607..3607
FT /note="SIT PstI"
FT misc_binding 3733..3733
FT /note="SIT PvuI"
XX
SQ Sequence 4892 BP; 1207 A; 1175 C; 1179 G; 1331 T; 0 other;
gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaccggatc
gatccctcga gtctagaccc ggggagctcg gatccagaca tgataagata cattgatgag
tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
ctctacaaat gtggtatggc tgattatgat cctctacgcc ggacgcatcg tggccggcat
caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
agatgacgac catcagggac agcttcaaag gccagcaaaa ggccaggaac cgtaaaaagg
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca tgtttgacag
cttatcatcg aagcagtagc aatcaaccca cacaagtgga tctttcctgt ataattttct
attttcatgc ttcatcctca gtaagcacag caagcatatg cagttagcag acattttctt
tgcacactca ggccattgtt tgcagtacat tgcatcaaca ccaggattta aggaagaagc
aaatacctca gttgcatccc agaagcctcc aaagtcaggt tgatgagcat attttactcc
atcttccatt ttcttgtaca gagtattcat tttcttcatt ttttcttcat ctcctccttt
atcaggatga aactccttgc atttttttaa atatgccttt ctcatcagag gaatattccc
ccaggcactc ctttcaagac ctagaaggtc cattagctgc aaagattcct ctctgtttaa
aactttatcc atctttgcaa agctttttgc aaaagcctag gcctccaaaa aagcctcctc
actacttctg gaatagctca gaggccgagg cg
//