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ID   PEX2       preliminary; circular DNA; SYN; 5787 BP.
XX
AC   X03174;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pEX2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5787
RC   pLK911 from pLK91
RC   pLK913 from pLK911
RC   pLK914 from pLK913 & pUC8
RC   pLK912 from pLK91 & pUC8
RC   pLK915 from pLK912 & pLK914
RC   pCL6191 from pCL19
RC   pCL919 from pCL6191 & pLK915
RC   pEX2 from pCL919
RC   pEX1, pEX3 from pEX2
RA   Stanley K.K., Luzio J.P.;
RT   "Construction of a new family of high efficiency bacterial
RT   expression vectors: identification of cDNA clones coding for human
RT   liver proteins";
RL   EMBO J. 3:1429-1434(1984).
XX
RN   [2]
RP   1-5787
RC   pEX2
RA   Stanley K.K.;
RT   ;
RL   Submitted (18-JUN-1986) on paper to the EMBL Data Library by:
RL   Stanley K.K., EMBL, Meyerhofstr. 1, Heidelberg, 6900, Germany.
XX
RN   [3]
RC   from pEX1 & pKT218
RA   Haymerle H., Herz J., Bressan G.M., Frank R., Stanley K.K.;
RT   "Efficient construction of cDNA libraries in plasmid expression
RT   vectors using an adaptor strategy";
RL   Nucleic Acids Res. 14:8615-8624(1986).
XX
RN   [4]
RC   pLK91 from pBR322 & phage fd terminator & STOP oligo
RA   Zabeau M.;
RT   ;
RL   Unpublished (1984).
XX
CC   NM (pEX2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)(CLONTECH)
CC   HO (E.coli POP2136)
CC   CP ()
CC   FN (expression)(screening cDNA)
CC   SE (color blue/white)
CC   PA (pCL919 from pLK915, from pLK91 & pUC8, & pCL6191, from pCL19)
CC   BR ()
CC   OF (pEX1)(pEX3)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ 2324bp
FT                   2. fd terminator 103bp in tandem
FT                   3. oligo, STOP
FT                   -> pLK91 2500bp
FT                   1. pLK91 EcoRI 2500bp, pBR322 4360..4360
FT                   Klenow
FT                   -> pLK911 2500bp [unique EcoRI at BamHI & HindIII]
FT                   1. pLK911 BamHI 2500bp, fd terminator
FT                   Klenow
FT                   -> pLK913 2500bp [no BamHI]
FT                   1. pLK913 remove EcoRI-HindIII 29bp, 2500bp
FT                   2. pUC8 EcoRI-HindIII 30bp 231..261
FT                   -> pLK914 2500bp [BamHI]
FT                   1. pLK91 remove MstI-XmnI 377bp 3591..3968, 2100bp
FT                   \ pBR322 MstI = 263 1359 1457 3591
FT                   \ pBR322 XmnI = 2036 3968
FT                   2. pUC8 XmnI-MstI 377bp 1052..1429, or 406..1052
FT                   -> pLK912 2500bp [no PstI]
FT                   1. pLK912 EcoRI-NdeI 247bp, MCS/pUC8 231..478
FT                   2. pLK914 NdeI-EcoRI 2061bp,
FT                   \ amp gene/pBR322 2299..4360
FT                   -> pLK915 2308bp [unique PstI]
FT                   1. lambda, pR/cro gene
FT                   -> pLK5
FT                   1. pLG400 8900bp
FT                   2. pLK5
FT                   -> pCL19
FT                   1. pCL19 BamHI
FT                   Klenow
FT                   -> pCL6191
FT                   1. pCL6191 EcoRI-EcoRI 3063bp
FT                   2. pLK915 EcoRI 2308bp
FT                   -> pCL919 5787bp
FT                   1. pCL919 EcoRI 5787bp 3197..3197
FT                   Klenow
FT                   -> pEX2 5787bp [unique EcoRI]"
FT   promoter        1..61
FT                   /note="PRO bacteriophage lambda PR"
FT   CDS             67..85
FT                   /note="GEN bacteriophage lambda cro gene"
FT   misc_feature    67..67
FT                   /note="ATG bacteriophage lambda cro gene
FT                   transcription start site"
FT   misc_feature    85..85
FT                   /note="TER bacteriophage lambda cro gene
FT                   amino terminus"
FT   misc_feature    111..135
FT                   /note="ADP oligonucleotide"
FT   promoter        136..249
FT                   /note="PRO E. coli lacI fragment"
FT   CDS             250..3195
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ),
FT                   amino acids 24-1005"
FT   misc_binding    1305..1305
FT                   /note="SIT unique EcoRV"
FT   misc_binding    1538..1538
FT                   /note="SIT unique BclI"
FT   misc_binding    2132..2132
FT                   /note="SIT unique SacI"
FT   misc_binding    3196..3231
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_feature    3197..3200
FT                   /note="ADP AATT deleted in pEX1"
FT   misc_feature    3200..3201
FT                   /note="ADP AATT inserted in pEX3"
FT   misc_feature    3234..3244
FT                   /note="TER stop codon region"
FT   misc_binding    3256..3256
FT                   /note="SIT unique XbaI"
FT   terminator      3269..3371
FT                   /note="TER bacteriophage fd transcription
FT                   terminator 1"
FT   terminator      3378..3480
FT                   /note="TER bacteriophage fd transcription
FT                   terminator 2"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5262..5262
FT                   /note="SIT unique ScaI"
XX
SQ   Sequence 5787 BP; 1382 A; 1511 C; 1541 G; 1353 T; 0 other;
     cgcaagggat aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
     ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga agcttgggat
     cgatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
     gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
     gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
     accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
     ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
     attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
     tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
     gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     ccgtcagtat cggcggaatt cccggggatc cgtcgacctg cagccaagct tgctgattga
     ttgaccggat cgatccggct ctagaattaa ttcacctcga aagcaagctg ataaaccgat
     acaattaaag gctccttttg gagccttttt ttttggagat tttcaacgtg aaaaaattat
     tattcgcaat tcaagctaat tcacctagaa agcaagctga taaaccgata caattaaagg
     ctccttttgg agcctttttt tttggagatt ttcaacgtga aaaaattatt attcgcaatt
     caagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     aggccctttc gtcttcaaga attaatt
//