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ID PEX3 preliminary; circular DNA; SYN; 5787 BP.
XX
AC IG1068; X03174;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pEX3 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-5787
RC pLK911 from pLK91
RC pLK913 from pLK911
RC pLK914 from pLK913 & pUC8
RC pLK912 from pLK91 & pUC8
RC pLK915 from pLK912 & pLK914
RC pCL6191 from pCL19
RC pCL919 from pCL6191 & pLK915
RC pEX2 from pCL919
RC pEX1, pEX3 from pEX2
RA Stanley K.K., Luzio J.P.;
RT "Construction of a new family of high efficiency bacterial
RT expression vectors: identification of cDNA clones coding for human
RT liver proteins";
RL EMBO J. 3:1429-1434(1984).
XX
RN [2]
RP 1-5787
RC pEX2
RA Stanley K.K.;
RT ;
RL Submitted (18-JUN-1986) on paper to the EMBL Data Library by:
RL Stanley K.K., EMBL, Meyerhofstr. 1, Heidelberg, 6900, Germany.
XX
CC Sequence length could be 5791 bp.
CC NM (pEX3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Boehringer)(CLONTECH)
CC HO (E.coli POP2136)
CC CP ()
CC FN (expression)(screening cDNA)
CC SE (color blue/white)
CC PA (pEX2)
CC BR (pEX1)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pEX2 EcoRI 5787bp 3197..3197
FT Klenow
FT -> pEX3 5787bp [no EcoRI, no SmaI]"
FT promoter 1..61
FT /note="PRO bacteriophage lambda PR"
FT CDS 67..85
FT /note="GEN bacteriophage lambda cro gene"
FT misc_feature 67..67
FT /note="ATG bacteriophage lambda cro gene
FT transcription start site"
FT misc_feature 85..85
FT /note="TER bacteriophage lambda cro gene
FT amino terminus"
FT misc_feature 111..135
FT /note="ADP oligonucleotide"
FT promoter 136..249
FT /note="PRO E. coli lacI fragment"
FT CDS 250..3195
FT /note="GEN E. coli beta-galactosidase gene (lacZ),
FT amino acids 24-1005"
FT misc_binding 1305..1305
FT /note="SIT unique EcoRV"
FT misc_binding 1538..1538
FT /note="SIT unique BclI"
FT misc_binding 2132..2132
FT /note="SIT unique SacI"
FT misc_binding 3196..3231
FT /note="MCS SmaI-BamHI-SalI-PstI-HindIII"
FT misc_feature 3197..3200
FT /note="ADP AATT deleted in pEX1"
FT misc_feature 3200..3201
FT /note="ADP AATT inserted in pEX3"
FT misc_feature 3234..3244
FT /note="TER synthetic stop codon region"
FT misc_binding 3256..3256
FT /note="SIT unique XbaI"
FT terminator 3269..3371
FT /note="TER bacteriophage fd transcription
FT terminator 1"
FT terminator 3378..3480
FT /note="TER bacteriophage fd transcription
FT terminator 2"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5262..5262
FT /note="SIT unique ScaI"
XX
SQ Sequence 5787 BP; 1382 A; 1511 C; 1541 G; 1353 T; 0 other;
cgcaagggat aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga agcttgggat
cgatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
ccgtcagtat cggcggaatt cccggggatc cgtcgacctg cagccaagct tgctgattga
ttgaccggat cgatccggct ctagaattaa ttcacctcga aagcaagctg ataaaccgat
acaattaaag gctccttttg gagccttttt ttttggagat tttcaacgtg aaaaaattat
tattcgcaat tcaagctaat tcacctagaa agcaagctga taaaccgata caattaaagg
ctccttttgg agcctttttt tttggagatt ttcaacgtga aaaaattatt attcgcaatt
caagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
aggccctttc gtcttcaaga attaatt
//