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ID   PEXCELL    preliminary; circular DNA; SYN; 4190 BP.
XX
AC   IG1069;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pExCell - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pExCell from pEMBL9 & lambda
RA   ;
RT   ;
RL   Unpublished (1992).
XX
CC   Vector size is 4277, but amount sequenced is 4190.
CC   NM (pExCell)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli NM522)(E.coli NP66)
CC   CP ()
CC   FN (expression)(transcription)
CC   SE (color blue/white)
CC   PA (lambda)(pEMBL9)
CC   BR (lambda ExCell)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL9+ 3939bp, f1 ori/lacZ/amp/ori
FT                   2. lambda BbvII/BbsI-StyI 308bp 27561..27869,
FT                   \ attL/attP/attR
FT                   -> pExCell 4190bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BbsI"
FT   misc_feature    0..0
FT                   /note="SIT bacteriophage lambda attL region"
FT   misc_feature    0..0
FT                   /note="SIT bacteriophage lambda attP region"
FT   misc_feature    0..0
FT                   /note="SIT bacteriophage lambda attR region"
FT   misc_binding    0..0
FT                   /note="SIT unique StyI"
FT   misc_binding    0..0
FT                   /note="SIT unique DsaI"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    0..0
FT                   /note="SIT unique AccI"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SwaI"
FT   misc_binding    0..0
FT                   /note="SIT unique DraIII"
FT   misc_binding    0..0
FT                   /note="SIT unique NaeI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS unique
FT                   HindIII-MluI-NotI-BamHI-EcoRI-SfiI-XhoI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage Sp6"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique BspHI"
FT   misc_binding    0..0
FT                   /note="SIT unique Eam1105I"
FT   misc_binding    0..0
FT                   /note="SIT unique BsaI"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique AhaII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   misc_binding    0..0
FT                   /note="SIT unique NheI"
XX
SQ   Sequence 4190 BP; 1066 A; 1007 C; 973 G; 1144 T; 0 other;
     cagatcgggg gtggacattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aatacaggcc
     tcgagggccg aattcagtct caggatccga cacggcggcc gcacgcgtaa gcttccctat
     agtgagtcgt attacgcaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     gattccgttg caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt
     ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg
     gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact
     tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc
     tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta
     cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct
     gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg
     attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt
     tgtagacctc tcaaaaatag ctaccctctc cggcatgaat ttatcagcta gaacggttga
     atatcatatt gatggtgatt tgactgtctc cggcctttct cacccgtttg aatctttacc
     tacacattac tcaggcattg catttaaaat atatgagggt tctaaaaatt tttatccttg
     cgttgaaata aaggcttctc ccgcaaaagt attacagggt cataatgttt ttggtacaac
     cgatttagct ttatgctctg aggctttatt gcttaatttt gctaattctt tgccttgcct
     gtatgattta ttggatgttg gaatcgcctg atgcggtatt ttctccttac gcatctgtgc
     ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     agccagcccc gacacccgcc aacacccgct gacgcgatgc ctacctttca cgagttgcgc
     agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     gggcataagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     aaaattgaaa tcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     ttgataagca atgctttttt ataatgccaa cttagtataa aaaagctgaa cgagaaacgt
     aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
     actgtaaaac acaacatatg cagtcactat gaatcaacta cttagatggt attagtgacc
     tgtaacagag cattagcgca aggtgatttt tgtcttcttg cgctaatttt ttgtcatcaa
     acctgtcgca ctccagagaa gcacaaagcc tcgcaatcca gtgcaaagct
//