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ID   PFH614     preliminary; circular DNA; SYN; 3957 BP.
XX
AC   K00455;
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DT   15-FEB-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFH614 - complete, pACYC177 amp/phix174 ori.
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KW   cloning vector; ampicillin resistance; drug resistance gene;
KW   origin of replication; plasmid.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-50
RC   pFH614, pFH704, pFH807, pFH812, pFH903 from pACYC177 & oligo, phiX174
RA   Heidekamp F., Baas P.D., Van Boom J.H., Veeneman G.H.,
RA   Zipursky S.L., Jansz H.S.;
RT   "Construction and characterization of recombinant plasmid DNAs
RT   containing sequences of the origin of bacteriophage Phi-X174 DNA
RT   replication";
RL   Nucleic Acids Res. 9:3335-3354(1981).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 208937
CC   NM (pFH614)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC177)(phi-x174 am3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC177 HincII 3941bp 4..4
FT                   2. oligo 16bp caacttgatattaata, phiX174 4299..4314
FT                   -> pFH614 3957bp"
FT   -               1..3
FT                   /note="pACYC177 1..3 3bp
FT                   HindII = HincII = GTY^RAC
FT                   \                     caact..."
FT   -               4..19
FT                   /note="caacttgatattaata 16bp
FT                   \            ...taata
FT                   HindII = HincII = GTY^RAC"
FT   -               20..3957
FT                   /note="pACYC177 4..3941 3938bp"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    11..11
FT                   /note="SIT HincII"
FT   rep_origin      11..42
FT                   /note="ORI phi-x174; head to tail repeat"
XX
SQ   Sequence 3957 BP; 1033 A; 895 C; 994 G; 1035 T; 0 other;
     gttcaacttg atattaatag acgccgggca agagcaactc ggtcgccgca tacactattc
     tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     cttaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc
     gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc
     acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa gcacttcact
     gacaccctca tcagtgccaa catagtaagc cagtatacac tccgctagcg ctgaggtctg
     cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag
     aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg
     aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc
     aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt
     tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
     ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
     gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt
     gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
     atgagtcagc aacaccttct tcacgaggca gacctcagcg ctcaaagatg caggggtaaa
     agctaaccgc atctttaccg acaaggcatc cggcagttca acagatcggg aagggctgga
     tttgctgagg atgaaggtgg aggaaggtga tgtcattctg gtgaagaagc tcgaccgtct
     tggccgcgac accgccgaca tgatccaact gataaaagag tttgatgctc agggtgtagc
     ggttcggttt attgacgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac
     catcctgtcg gctgtggcac aggctgaacg ccggaggatc ctagagcgca cgaatgaggg
     ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa
     cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag
     tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta
     tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgt
//