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ID   PFH704     preliminary; circular DNA; SYN; 3957 BP.
XX
AC   K00452;
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DT   15-FEB-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFH704 - complete, pACYC177 kmr/phix174 ori.
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KW   cloning vector; drug resistance gene; kanamycin resistance;
KW   origin of replication; plasmid.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-50
RC   pFH614, pFH704, pFH807, pFH812, pFH903 from pACYC177 & oligo, phiX174
RA   Heidekamp F., Baas P.D., Van Boom J.H., Veeneman G.H.,
RA   Zipursky S.L., Jansz H.S.;
RT   "Construction and characterization of recombinant plasmid DNAs
RT   containing sequences of the origin of bacteriophage Phi-X174 DNA
RT   replication";
RL   Nucleic Acids Res. 9:3335-3354(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 208934
CC   NM (pFH704)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC177)(phi-x174 am3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC177 SmaI 3941bp 2229..2229
FT                   2. oligo 16bp caacttgatattaata, phiX174 4299..4314
FT                   -> pFH704 3957bp"
FT   -               1..2228
FT                   /note="pACYC177 1..2228 2228bp
FT                   SmaI = CCC^GGG
FT                   \          caact..."
FT   -               2229..2244
FT                   /note="caacttgatattaata 16bp
FT                   \ ...taata
FT                   SmaI = CCC^GGG"
FT   -               2245..3957
FT                   /note="pACYC177 2229..3941 1713bp"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   misc_binding    11..11
FT                   /note="SIT SmaI"
FT   rep_origin      11..42
FT                   /note="ORI phi-x174; tail to tail repeat"
XX
SQ   Sequence 3957 BP; 1033 A; 895 C; 994 G; 1035 T; 0 other;
     gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
     tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
     cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
     ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
     aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
     ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
     ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
     ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
     cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
     atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     cgatccccca acttgatatt aatagggaaa acagcattcc aggtattaga agaatatcct
     gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt
     gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
     atgagtcagc aacaccttct tcacgaggca gacctcagcg ctcaaagatg caggggtaaa
     agctaaccgc atctttaccg acaaggcatc cggcagttca acagatcggg aagggctgga
     tttgctgagg atgaaggtgg aggaaggtga tgtcattctg gtgaagaagc tcgaccgtct
     tggccgcgac accgccgaca tgatccaact gataaaagag tttgatgctc agggtgtagc
     ggttcggttt attgacgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac
     catcctgtcg gctgtggcac aggctgaacg ccggaggatc ctagagcgca cgaatgaggg
     ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa
     cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag
     tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta
     tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgt
//