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ID PFH704 preliminary; circular DNA; SYN; 3957 BP.
XX
AC K00452;
XX
DT 15-FEB-1984 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pFH704 - complete, pACYC177 kmr/phix174 ori.
XX
KW cloning vector; drug resistance gene; kanamycin resistance;
KW origin of replication; plasmid.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-50
RC pFH614, pFH704, pFH807, pFH812, pFH903 from pACYC177 & oligo, phiX174
RA Heidekamp F., Baas P.D., Van Boom J.H., Veeneman G.H.,
RA Zipursky S.L., Jansz H.S.;
RT "Construction and characterization of recombinant plasmid DNAs
RT containing sequences of the origin of bacteriophage Phi-X174 DNA
RT replication";
RL Nucleic Acids Res. 9:3335-3354(1981).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NCBI gi: 208934
CC NM (pFH704)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pACYC177)(phi-x174 am3)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pACYC177 SmaI 3941bp 2229..2229
FT 2. oligo 16bp caacttgatattaata, phiX174 4299..4314
FT -> pFH704 3957bp"
FT - 1..2228
FT /note="pACYC177 1..2228 2228bp
FT SmaI = CCC^GGG
FT \ caact..."
FT - 2229..2244
FT /note="caacttgatattaata 16bp
FT \ ...taata
FT SmaI = CCC^GGG"
FT - 2245..3957
FT /note="pACYC177 2229..3941 1713bp"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
FT misc_binding 11..11
FT /note="SIT SmaI"
FT rep_origin 11..42
FT /note="ORI phi-x174; tail to tail repeat"
XX
SQ Sequence 3957 BP; 1033 A; 895 C; 994 G; 1035 T; 0 other;
gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
cgatccccca acttgatatt aatagggaaa acagcattcc aggtattaga agaatatcct
gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt
gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
atgagtcagc aacaccttct tcacgaggca gacctcagcg ctcaaagatg caggggtaaa
agctaaccgc atctttaccg acaaggcatc cggcagttca acagatcggg aagggctgga
tttgctgagg atgaaggtgg aggaaggtga tgtcattctg gtgaagaagc tcgaccgtct
tggccgcgac accgccgaca tgatccaact gataaaagag tttgatgctc agggtgtagc
ggttcggttt attgacgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac
catcctgtcg gctgtggcac aggctgaacg ccggaggatc ctagagcgca cgaatgaggg
ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa
cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag
tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta
tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgt
//