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ID   PFL2       preliminary; circular DNA; SYN; 7770 BP.
XX
AC   IG8004;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pFL2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pFL1, pFL2, pFL3, pFL4 from pBR322 & yeast 2 micron
RA   Chevalier M.R., Bloch J.C., Lacroute F.;
RT   "Transcriptional and translational expression of a chimeric
RT   bacterial-yeast plasmid in yeasts";
RL   Gene 11:11-19(1980).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pFL2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR (yeast 2 micron)(pBR322)(YEp24)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII 4361bp 30..30
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pBR322-6 5527bp
FT                   1. pBR322-6 EcoRI 5527bp, pBR322 4360..4360
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2, D fragment
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pFL2 7762bp"
FT   -               1..29
FT                   /note="pBR322 1..29 29bp
FT                   HindIII = A^AGCTT"
FT   -               30..1195
FT                   /note="YEp24 2273..3438 1166bp
FT                   HindIII = A^AGCTT"
FT   -               1196..5525
FT                   /note="pBR322 30..4359 4330bp
FT                   EcoRI = G^AATTC"
FT   -               5526..7059
FT                   /note="yeast 2 micron 2408..3941 1534bp
FT                   EcoRI = G^AATTC"
FT   -               7060..7768
FT                   /note="yeast 2 micron 6316..6318..706
FT                   \ 709bp complement
FT                   EcoRI = G^AATTC"
FT   -               7769..7770
FT                   /note="pBR322 4360..4361 2bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
XX
SQ   Sequence 7770 BP; 1997 A; 1837 C; 1838 G; 2098 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttttcaat tcaattcatc attttttttt
     tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
     aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
     aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
     gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
     cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
     ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
     aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
     cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     tcaagaattc cacggactat agactatact agtatactcc gtctactgta cgatacactt
     ccgctcaggt ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca
     gctcagcaaa ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga
     ccgagaaaga gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc
     gatgtgacgc tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat
     atccgacaaa ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa
     catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat
     atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca
     tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt
     gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     agcgcttttg gttttcaaaa gcgctctgaa gttcctatat agagaatagg aacttcggaa
     taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga
     aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc
     cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc
     cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt
     tatagcaaag attgaataag gcgcattttt cttcaaagct ttattgtacg atctgactaa
     gttatctttt aataattggt attcctgttt attgcttgaa gaattgccgg tcctatttac
     tcgttttagg actggttcag aattcctcaa
//