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ID   PFL26      preliminary; circular DNA; SYN; 5558 BP.
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AC   X70477; ATCC77199;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pFL26 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5558
RC   pFL26
RA   Ozier-Kalogeropoulos O.;
RT   ;
RL   Submitted (01-JUN-1993) by:
RL   Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
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RN   [2]
RP   1-5558
RC   plasmid from pUC19 & linkers
RC   pFL26 from plasmid & YEp13, LEU2 gene
RC   pFL34 from plasmid & pBR322-URA3, URA3 gene
RC   pFL35 from plasmid & YRp7, TRP1 gene
RC   pFL36 from pFL26 & CEN6 & ARS
RC   pFL38 from pFL34 & CEN6 & ARS
RC   pFL39 from pFL35 & CEN6 & ARS
RC   pFL46S from pFL26 & yeast 2 micron & linker
RC   pFL44S from pFL34 & yeast 2 micron & linker
RC   pFL45S from pFL35 & yeast 2 micron & linker
RC   pFL46L from pFL26 & pFL1 & linker
RC   pFL44L from pFL34 & pFL1 & linker
RC   pFL45L from pFL35 & pFL1 & linker
RC   pFL59- from pFL39 & pGEM-3Zf-
RC   pFL59+ from pFL44S & pGEM-3Zf+
RC   pFL64- from pFL39 & pGEM-3Zf-
RC   pFL64+ from pFL44S & pGEM-3Zf+
RA   Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA   Minvielle-Sebastia L., Lacroute F.;
RT   "A family of low and high copy replicative, integrative and
RT   single-stranded S. cerevisiae/E. coli shuttle vectors";
RL   Yeast 7:609-615(1991).
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CC   pFL26 was constructed from pUC19 plasmid where two alu I sites
CC   were modified.The site 629 was replaced by a BglII linker and the
CC   site 747 by a ClaI site. The yeast selectable marker has been
CC   cloned in the BglII site.
CC
CC   The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC   LEU2 - pMB1 ori - ampR.
CC   YI-type shuttle integration vector permitting visual detection of
CC   recombinants based on alpha complementation.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.6; BglII--2.8 (doublet); ClaI--3.6, 1.9. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 397127
CC   NM (pFL26)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(yeast 2-micron plasmid)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 AluI 2686bp 631..631
FT                   BglII linker 6bp agatct
FT                   AluI 2693bp 747..747
FT                   ClaI linker 6bp atcgat
FT                   -> plasmid 2703bp
FT                   1. plasmid BglII 2703bp 630..630
FT                   2. YEp13 BglII-BglII 2855bp, yeast LEU2 gene
FT                   -> pFL26 5558bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT                   XmaI-BamHI-XbaI-
FT                   AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SmaI-XmaI-BamHI-XbaI-PstI-
FT                   SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
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SQ   Sequence 5558 BP; 1586 A; 1218 C; 1251 G; 1503 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag cagatctatt acattatggg tggtatgttg
     gaataaaaat caactatcat ctactaacta gtatttacgt tactagtata ttatcatata
     cggtgttaga agatgacgca aatgatgaga aatagtcatc taaattagtg gaagctgaaa
     cgcaaggatt gataatgtaa taggatcaat gaatattaac atataaaatg atgataataa
     tatttataga attgtgtaga attgcagatt cccttttatg gattcctaaa tcctcgagga
     gaacttctag tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca
     attacatcaa aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg
     tgttcaaaaa cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt
     gtttggccga gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg
     ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt
     cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
     aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
     gagaaaaagg aaaggtgaga gccgcggaac cggcttttca tatagaatag agaagcgttc
     atgactaaat gcttgcatca caatacttga agttgacaat attatttaag gacctattgt
     tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt
     cagcaatata tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga
     tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc
     ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg
     gtggtgctgc tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca
     agaaggttga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg
     ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa
     gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg
     ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga
     gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag
     tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta
     tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg
     aggaaaccat caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg
     ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca
     tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc
     catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat
     gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt
     ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag
     atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca
     gtaccacgga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag
     attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg
     acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac
     atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat
     actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga
     ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt
     tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg
     acctgaccat ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga
     aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagt cgacctcctc
     ttcagtacta aatctaccaa taccaaatct gatggaagaa tgggctaatg catcatcctt
     acccagcgca tgtaaaacat aagaaggttc tagggaagca gatgtacagg ctgaacccga
     ggataatgcg atatccctta gtgccatcaa taaagattct ccttccacgt aggcgaaaga
     aacgttaaca caccctggat aacgatgatc tggagatccg ttcaacgtgg tatgttcagc
     ggataataga cctttgacta atttatcgga tagtcttttg atgtgagctt ggtcgttgtc
     aaattctttc ttcatcaatc tcgcagcttc accaaatccc gctaccaatg ggggggccaa
     agtaccagat ctgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gcatcgatgc tcactcaaag gtcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     aacctataaa aataggcgta tcacgaggcc ctttcgtc
//