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ID   PFL261     preliminary; circular DNA; SYN; 4589 BP.
XX
AC   M29363;
XX
DT   15-JAN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFL261 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4589
RC   pFL200 from pFL114
RC   pFL236 from pFL200 & pFL166
RC   pFL245 from pFL200
RC   pFL257 from pFL236
RC   pFL260 from pFL245
RC   pFL261 from pFL257
RA   Larimer F.W., Mural R.J., Soper T.S.;
RT   "Versatile protein engineering vectors for mutagenesis,
RT   expression and hybrid enzyme formation";
RL   Protein Engineering 3:227-231(1990).
XX
RN   [2]
RC   pFL261
RA   Larimer F.W.;
RT   ;
RL   Submitted (23-OCT-1989) to GenBank on computer-readable media by:
RL   Larimer F.W., ORNL, PO Box 2009, Oak Ridge TN 37831, USA.
XX
CC   NM (pFL261)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (mutagenesis)(expression)(fusion)
CC   SE ()
CC   PA (p15A)
CC   BR (pFL260)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove HindIII-BamHI 346bp
FT                   \ 1525..1871, 3899bp
FT                   2. E.coli HindIII-BamHI, tac prom/rbc gene
FT                   -> pFL166
FT                   1. pFL166 remove XbaI-HindIII
FT                   2. pFL200 AatII
FT                   XbaI linker 6bp tctaga
FT                   XbaI-HindIII 1240bp, f1 ori
FT                   -> pFL236
FT                   1. pFL236 remove 17bp aggaaacaggatccatg, RBS-ATG
FT                   2. oligo 17bp aggaggaaggatccatg, RBS-ATG
FT                   -> pFL257
FT                   1. pFL257 remove NcoI-EagI, rbc gene
FT                   -> pFL261 4589bp"
FT   promoter        318..345
FT                   /note="PRO E. coli tac (trp and lac)"
FT   RBS             380..384
FT                   /note="RBS"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   rep_origin      3061..3061
FT                   /note="ORI E. coli miniplasmid p15A"
XX
SQ   Sequence 4589 BP; 1133 A; 1185 C; 1202 G; 1069 T; 0 other;
     gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc
     tgcaattcag agcgccagca agtgggggac agcagaagac ctgaccgccg cagagtggat
     gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc
     taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct
     gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaagct
     tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
     cggataacaa tttcacacag gaggaaggat ccatgggtcg accggccgac gcgctgggct
     acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
     gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
     tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     ttcgtaaagt ctggaaacgc ggaagtcccc tacgtgctgc tgaagttgcc cgcaacagag
     agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct
     catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc
     gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag
     gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
     ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
     cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
     aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccattgt
     gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
     aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
     ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
     gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
     tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
     ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
     gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
     tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
     ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
     ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
     ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg
     ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     tctcaagaag atcatcttat taatcagata aaatatttct agagctaaga aaccattatt
     atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta
     atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag
     ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     gtgaattgta atacgactca ctatagggc
//