ID   PFL281     preliminary; circular DNA; SYN; 3582 BP.
XX
AC   M68946;
XX
DT   05-JUN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pFL281 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3582
RC   pFL281 from pFL series
RA   Larimer F.W.;
RT   ;
RL   Unpublished (1991).
XX
CC   NM (pFL281)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFL261 remove, 4589bp-
FT                   -> pFL281 3582bp"
FT   promoter        318..345
FT                   /note="PRO E. coli tac (trc and lac) [1,2]"
FT   RBS             380..384
FT                   /note="RBS [1,2], putative"
FT   misc_feature    387..409
FT                   /note="MCS [1,2]"
FT   rep_origin      882..883
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1642..2505)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), partial [1,2]"
FT   misc_feature    complement(2939..3394)
FT                   /note="ORI bacteriophage f1 intergenic region [1,2]"
XX
SQ   Sequence 3582 BP; 914 A; 891 C; 946 G; 831 T; 0 other;
     gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc
     tgcaattcag agcgccagca agtgggggac agcagaagac ctgaccgccg cagagtggat
     gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc
     taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct
     gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaagct
     tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
     cggataacaa tttcacacag gaggaaggat ccatgggtcg accggccgcg atccgctgcc
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc
     aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gc
//