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ID   PFL36      preliminary; circular DNA; SYN; 6369 BP.
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AC   X70481; ATCC77202;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pFL36 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-6369
RC   pFL36
RA   Ozier-Kalogeropoulos O.;
RT   ;
RL   Submitted (01-JUN-1993) by:
RL   Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
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RN   [2]
RP   1-6369
RC   plasmid from pUC19 & linkers
RC   pFL26 from plasmid & YEp13, LEU2 gene
RC   pFL34 from plasmid & pBR322-URA3, URA3 gene
RC   pFL35 from plasmid & YRp7, TRP1 gene
RC   pFL36 from pFL26 & CEN6 & ARS
RC   pFL38 from pFL34 & CEN6 & ARS
RC   pFL39 from pFL35 & CEN6 & ARS
RC   pFL46S from pFL26 & yeast 2 micron & linker
RC   pFL44S from pFL34 & yeast 2 micron & linker
RC   pFL45S from pFL35 & yeast 2 micron & linker
RC   pFL46L from pFL26 & pFL1 & linker
RC   pFL44L from pFL34 & pFL1 & linker
RC   pFL45L from pFL35 & pFL1 & linker
RC   pFL59- from pFL39 & pGEM-3Zf-
RC   pFL59+ from pFL44S & pGEM-3Zf+
RC   pFL64- from pFL39 & pGEM-3Zf-
RC   pFL64+ from pFL44S & pGEM-3Zf+
RA   Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA   Minvielle-Sebastia L., Lacroute F.;
RT   "A family of low and high copy replicative, integrative and
RT   single-stranded S. cerevisiae/E. coli shuttle vectors";
RL   Yeast 7:609-615(1991).
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RN   [3]
RP   3613-4424
RC   pLA427 from YRp7, CEN6
RC   pLA406, pLA417, pLA418, pLA429 from pLA427
RA   Panzeri L., Philippsen P.;
RT   "Centromeric DNA from chromosome VI in Saccharomyces cerevisiae
RT   strains";
RL   EMBO J. 1:1605-1611(1982).
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CC   pFL36 was constructed from pUC19 plasmid where two alu I sites
CC   were modified. The site 629 was replaced by a BglII linker and the
CC   site 747 by a ClaI site. The yeast selectable marker has been
CC   cloned in the BglII site and the yeast replication origin in the
CC   ClaI site.
CC
CC   The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC   LEU2 - ARS-CEN6 - pMB1 ori - ampR.
CC   YC-type low copy number shuttle vector permitting visual detection of
CC   recombinants based on alpha complementation.
CC   The loss per generation (percentage having lost all the plasmids after
CC   20 h of non-selective growth) is 8.3.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.6; BglII--3.7, 2.9; ClaI--3.9, 1.9, 0.8. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 397129
CC   NM (pFL36)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(yeast 2-micron plasmid)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast TaqI-TaqI 400bp, CEN6
FT                   2. yeast ClaI-ClaI 400bp, ARS
FT                   -> fragment 800bp
FT                   1. fragment ClaI 800bp, between ARS and CEN6
FT                   fill in
FT                   -> fragment2 800bp
FT                   1. pFL26 ClaI 5558bp 1694..1694, may be 3615..3615
FT                   2. fragment2 TaqI-ClaI 811bp, ARS/CEN6
FT                   -> pFL36 6369bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT                   XmaI-BamHI-XbaI-
FT                   AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SmaI-XmaI-BamHI-XbaI-PstI-
FT                   SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN6"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
XX
SQ   Sequence 6369 BP; 1864 A; 1334 C; 1347 G; 1824 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag cagatctatt acattatggg tggtatgttg
     gaataaaaat caactatcat ctactaacta gtatttacgt tactagtata ttatcatata
     cggtgttaga agatgacgca aatgatgaga aatagtcatc taaattagtg gaagctgaaa
     cgcaaggatt gataatgtaa taggatcaat gaatattaac atataaaatg atgataataa
     tatttataga attgtgtaga attgcagatt cccttttatg gattcctaaa tcctcgagga
     gaacttctag tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca
     attacatcaa aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg
     tgttcaaaaa cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt
     gtttggccga gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg
     ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt
     cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
     aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
     gagaaaaagg aaaggtgaga gccgcggaac cggcttttca tatagaatag agaagcgttc
     atgactaaat gcttgcatca caatacttga agttgacaat attatttaag gacctattgt
     tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt
     cagcaatata tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga
     tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc
     ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg
     gtggtgctgc tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca
     agaaggttga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg
     ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa
     gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg
     ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga
     gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag
     tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta
     tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg
     aggaaaccat caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg
     ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca
     tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc
     catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat
     gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt
     ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag
     atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca
     gtaccacgga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag
     attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg
     acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac
     atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat
     actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga
     ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt
     tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg
     acctgaccat ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga
     aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagt cgacctcctc
     ttcagtacta aatctaccaa taccaaatct gatggaagaa tgggctaatg catcatcctt
     acccagcgca tgtaaaacat aagaaggttc tagggaagca gatgtacagg ctgaacccga
     ggataatgcg atatccctta gtgccatcaa taaagattct ccttccacgt aggcgaaaga
     aacgttaaca caccctggat aacgatgatc tggagatccg ttcaacgtgg tatgttcagc
     ggataataga cctttgacta atttatcgga tagtcttttg atgtgagctt ggtcgttgtc
     aaattctttc ttcatcaatc tcgcagcttc accaaatccc gctaccaatg ggggggccaa
     agtaccagat ctgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gcatcgatga atggaaatta cgtactttca acgcaagagc aagacaaaag
     aaagcctaaa actgtctttt cgtgtcggtc gtccaatatc atcgtaaacg tgtgtagagc
     cttaaattct aatttctaac tttaaatttt ttttgctatt ataatactaa tttctaactt
     tcaacctatt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
     tttctatttt taatttatat atttatatta aaaaatttaa attatattat ttttatagca
     cgtgatgaaa agaaatatag tcttcaaggt tatattatac aggtttatat attattttac
     ttatttttgt tgttcttttt cttggagtaa tttagcatta acaacttcga cccatcgcga
     tgacgaaatt gcacgtttaa aggagaagtc ggaatcgttg caagaagaaa taaattagtt
     tgacgtttta ttccttttct tctgcacaga agtgtgtata tatatattta tatatagtcg
     atgtgcaaaa gcctacatta tttttctttt gatttttatt tttttgttca agtaggttca
     tgccgacagt aaatccttat gccctaccta aatataaaca cccaaatata tatatatata
     tatatatata taaataataa aaacggaaag gaaatagaat aataataata atactacacg
     tttacgtatg taactcattc tattattcag actttgatat taatagaatt attactacta
     ggtttcctca cttcaaaatc tataaatata ccgtcatgct ctaatcgatg ctcactcaaa
     ggtcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     cctttcgtc
//