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ID PFL39 preliminary; circular DNA; SYN; 4354 BP.
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AC X70483; ATCC77204;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector pFL39 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4354
RC pFL39
RA Ozier-Kalogeropoulos O.;
RT ;
RL Submitted (01-JUN-1993) by:
RL Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
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RN [2]
RP 1-4354
RC plasmid from pUC19 & linkers
RC pFL26 from plasmid & YEp13, LEU2 gene
RC pFL34 from plasmid & pBR322-URA3, URA3 gene
RC pFL35 from plasmid & YRp7, TRP1 gene
RC pFL36 from pFL26 & CEN6 & ARS
RC pFL38 from pFL34 & CEN6 & ARS
RC pFL39 from pFL35 & CEN6 & ARS
RC pFL46S from pFL26 & yeast 2 micron & linker
RC pFL44S from pFL34 & yeast 2 micron & linker
RC pFL45S from pFL35 & yeast 2 micron & linker
RC pFL46L from pFL26 & pFL1 & linker
RC pFL44L from pFL34 & pFL1 & linker
RC pFL45L from pFL35 & pFL1 & linker
RC pFL59- from pFL39 & pGEM-3Zf-
RC pFL59+ from pFL44S & pGEM-3Zf+
RC pFL64- from pFL39 & pGEM-3Zf-
RC pFL64+ from pFL44S & pGEM-3Zf+
RA Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA Minvielle-Sebastia L., Lacroute F.;
RT "A family of low and high copy replicative, integrative and
RT single-stranded S. cerevisiae/E. coli shuttle vectors";
RL Yeast 7:609-615(1991).
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RN [3]
RP 1598-2409
RC pLA427 from YRp7, CEN6
RC pLA406, pLA417, pLA418, pLA429 from pLA427
RA Panzeri L., Philippsen P.;
RT "Centromeric DNA from chromosome VI in Saccharomyces cerevisiae
RT strains";
RL EMBO J. 1:1605-1611(1982).
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CC pFL39 was constructed from pUC19 plasmid where two alu I sites
CC were modified. The site 629 was replaced by a BglII linker and the
CC site 747 by a ClaI site. The yeast selectable marker has been
CC cloned in the BglII site and the yeast replication origin in the
CC ClaI site.
CC
CC The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC TRP1 - ARS-CEN6 - pMB1 ori - ampR.
CC YC-type low copy number shuttle vector permitting visual detection of
CC recombinants based on alpha complementation.
CC The loss per generation (percentage having lost all the plasmids after
CC 20 h of non-selective growth) is 11.0.
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--4.6; BglII--3.7, 0.85; ClaI--3.75, 0.8. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NCBI gi: 397131
CC NM (pFL39)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUC19)(yeast 2-micron plasmid)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. yeast TaqI-TaqI 400bp, CEN6
FT 2. yeast ClaI-ClaI 400bp, ARS
FT -> fragment 800bp
FT 1. fragment ClaI 800bp, between ARS and CEN6
FT fill in
FT -> fragment2 800bp
FT 1. pFL35 ClaI 3543bp 1600..1600
FT 2. fragment2 TaqI-ClaI 811bp, ARS/CEN6
FT -> pFL39 4354bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT XmaI-BamHI-XbaI-
FT AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT AccI-HincII-SalI-PstI-SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_feature 0..0
FT /note="yeast centromere CEN6"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast TRP1 gene"
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SQ Sequence 4354 BP; 1189 A; 1009 C; 922 G; 1234 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag cagatctggg caagtgcaca aacaatactt
aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt
gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc
atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg
taaactttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag
ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga
agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa
accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatatcaat
gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa acacgccaac
caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg aaattttcct
tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac ccagcaagtc
agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc aaactttcac
caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg cctttgtgtg
cttaatcacg gtatatatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc
tctccttttc ttttttcgac cgaattaatt cagatctgct gcattaatga atcggccaac
gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
tgcgctcggt cgttcggctg cggcgagcgg tatcagcatc gatgaatgga aattacgtac
tttcaacgca agagcaagac aaaagaaagc ctaaaactgt cttttcgtgt cggtcgtcca
atatcatcgt aaacgtgtgt agagccttaa attctaattt ctaactttaa attttttttg
ctattataat actaatttct aactttcaac ctattttaca tcttcggaaa acaaaaacta
ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
tttaaattat attattttta tagcacgtga tgaaaagaaa tatagtcttc aaggttatat
tatacaggtt tatatattat tttacttatt tttgttgttc tttttcttgg agtaatttag
cattaacaac ttcgacccat cgcgatgacg aaattgcacg tttaaaggag aagtcggaat
cgttgcaaga agaaataaat tagtttgacg ttttattcct tttcttctgc acagaagtgt
gtatatatat atttatatat agtcgatgtg caaaagccta cattattttt cttttgattt
ttattttttt gttcaagtag gttcatgccg acagtaaatc cttatgccct acctaaatat
aaacacccaa atatatatat atatatatat atatataaat aataaaaacg gaaaggaaat
agaataataa taataatact acacgtttac gtatgtaact cattctatta ttcagacttt
gatattaata gaattattac tactaggttt cctcacttca aaatctataa atataccgtc
atgctctaat cgatgctcac tcaaaggtcg gtaatacggt tatccacaga atcaggggat
aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
tataaaaata ggcgtatcac gaggcccttt cgtc
//