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ID   PFL39      preliminary; circular DNA; SYN; 4354 BP.
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AC   X70483; ATCC77204;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pFL39 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4354
RC   pFL39
RA   Ozier-Kalogeropoulos O.;
RT   ;
RL   Submitted (01-JUN-1993) by:
RL   Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
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RN   [2]
RP   1-4354
RC   plasmid from pUC19 & linkers
RC   pFL26 from plasmid & YEp13, LEU2 gene
RC   pFL34 from plasmid & pBR322-URA3, URA3 gene
RC   pFL35 from plasmid & YRp7, TRP1 gene
RC   pFL36 from pFL26 & CEN6 & ARS
RC   pFL38 from pFL34 & CEN6 & ARS
RC   pFL39 from pFL35 & CEN6 & ARS
RC   pFL46S from pFL26 & yeast 2 micron & linker
RC   pFL44S from pFL34 & yeast 2 micron & linker
RC   pFL45S from pFL35 & yeast 2 micron & linker
RC   pFL46L from pFL26 & pFL1 & linker
RC   pFL44L from pFL34 & pFL1 & linker
RC   pFL45L from pFL35 & pFL1 & linker
RC   pFL59- from pFL39 & pGEM-3Zf-
RC   pFL59+ from pFL44S & pGEM-3Zf+
RC   pFL64- from pFL39 & pGEM-3Zf-
RC   pFL64+ from pFL44S & pGEM-3Zf+
RA   Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA   Minvielle-Sebastia L., Lacroute F.;
RT   "A family of low and high copy replicative, integrative and
RT   single-stranded S. cerevisiae/E. coli shuttle vectors";
RL   Yeast 7:609-615(1991).
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RN   [3]
RP   1598-2409
RC   pLA427 from YRp7, CEN6
RC   pLA406, pLA417, pLA418, pLA429 from pLA427
RA   Panzeri L., Philippsen P.;
RT   "Centromeric DNA from chromosome VI in Saccharomyces cerevisiae
RT   strains";
RL   EMBO J. 1:1605-1611(1982).
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CC   pFL39 was constructed from pUC19 plasmid where two alu I sites
CC   were modified. The site 629 was replaced by a BglII linker and the
CC   site 747 by a ClaI site. The yeast selectable marker has been
CC   cloned in the BglII site and the yeast replication origin in the
CC   ClaI site.
CC
CC   The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC   TRP1 - ARS-CEN6 - pMB1 ori - ampR.
CC   YC-type low copy number shuttle vector permitting visual detection of
CC   recombinants based on alpha complementation.
CC   The loss per generation (percentage having lost all the plasmids after
CC   20 h of non-selective growth) is 11.0.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.6; BglII--3.7, 0.85; ClaI--3.75, 0.8. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 397131
CC   NM (pFL39)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(yeast 2-micron plasmid)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast TaqI-TaqI 400bp, CEN6
FT                   2. yeast ClaI-ClaI 400bp, ARS
FT                   -> fragment 800bp
FT                   1. fragment ClaI 800bp, between ARS and CEN6
FT                   fill in
FT                   -> fragment2 800bp
FT                   1. pFL35 ClaI 3543bp 1600..1600
FT                   2. fragment2 TaqI-ClaI 811bp, ARS/CEN6
FT                   -> pFL39 4354bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT                   XmaI-BamHI-XbaI-
FT                   AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT                   AccI-HincII-SalI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN6"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
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SQ   Sequence 4354 BP; 1189 A; 1009 C; 922 G; 1234 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag cagatctggg caagtgcaca aacaatactt
     aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt
     gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc
     atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg
     taaactttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag
     ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga
     agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa
     accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatatcaat
     gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa acacgccaac
     caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg aaattttcct
     tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac ccagcaagtc
     agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc aaactttcac
     caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg cctttgtgtg
     cttaatcacg gtatatatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc
     tctccttttc ttttttcgac cgaattaatt cagatctgct gcattaatga atcggccaac
     gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagcatc gatgaatgga aattacgtac
     tttcaacgca agagcaagac aaaagaaagc ctaaaactgt cttttcgtgt cggtcgtcca
     atatcatcgt aaacgtgtgt agagccttaa attctaattt ctaactttaa attttttttg
     ctattataat actaatttct aactttcaac ctattttaca tcttcggaaa acaaaaacta
     ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
     tttaaattat attattttta tagcacgtga tgaaaagaaa tatagtcttc aaggttatat
     tatacaggtt tatatattat tttacttatt tttgttgttc tttttcttgg agtaatttag
     cattaacaac ttcgacccat cgcgatgacg aaattgcacg tttaaaggag aagtcggaat
     cgttgcaaga agaaataaat tagtttgacg ttttattcct tttcttctgc acagaagtgt
     gtatatatat atttatatat agtcgatgtg caaaagccta cattattttt cttttgattt
     ttattttttt gttcaagtag gttcatgccg acagtaaatc cttatgccct acctaaatat
     aaacacccaa atatatatat atatatatat atatataaat aataaaaacg gaaaggaaat
     agaataataa taataatact acacgtttac gtatgtaact cattctatta ttcagacttt
     gatattaata gaattattac tactaggttt cctcacttca aaatctataa atataccgtc
     atgctctaat cgatgctcac tcaaaggtcg gtaatacggt tatccacaga atcaggggat
     aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     tataaaaata ggcgtatcac gaggcccttt cgtc
//