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ID   PFL40      preliminary; circular DNA; SYN; 4303 BP.
XX
AC   IG9874;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pFL40 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pUC19 & linkers
RC   pFL34 from plasmid & URA3 gene
RC   pFL40 from pFL34 & yeast 2 micron ori
RC   pFL60 from pFL40 & PGK promoter/terminator & linker
RA   Minet M., Lacroute F.;
RT   "Cloning and sequencing of a human cDNA coding for a multifunctional
RT   polypeptide of the purine pathway by complementation of the ade2-101
RT   mutant in Saccharomyces cerevisiae";
RL   Curr. Genet. 18:287-291(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pFL40)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pFL34)(yeast 2 micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFL34 ClaI 3799bp 1856..1856
FT                   2. yeast 2 micron B form MstI-MstI 500bp
FT                   \ 3516..3942 & 703..629, ori
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pFL40 4299bp
FT                   \ [AccI, HincII, SmaI, XbaI: not unique]"
FT   -               1..1857
FT                   /note="pFL34 1..1857 1857bp
FT                   ClaI = AT^CG AT
FT                   MstI =   TGC^GCA"
FT   -               1858..2284
FT                   /note="yeast 2 micron 3516..3942 427bp"
FT   -               2285..2359
FT                   /note="yeast 2 micron 629..703 75bp complement
FT                   MstI = TGC^GCA
FT                   ClaI =  AT^CGAT"
FT   -               2360..4303
FT                   /note="pFL34 1856..3799 1944bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT                   XmaI-BamHI-XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique NotI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 4303 BP; 1121 A; 1013 C; 982 G; 1187 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag cagatctgag ctttttcttt ccaatttttt
     ttttttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
     cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
     catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
     ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
     caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
     caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
     attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
     gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
     caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
     gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
     acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
     tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
     attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
     tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
     ctttcgacat gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac
     tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
     ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgctgcat
     taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agcatcggca
     tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
     ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
     tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
     agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
     atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
     atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
     ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
     atacagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     aaaatgcaac gcgagctgcc gatgctcact caaaggtcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//