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ID PFL40 preliminary; circular DNA; SYN; 4303 BP.
XX
AC IG9874;
XX
DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector pFL40 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC plasmid from pUC19 & linkers
RC pFL34 from plasmid & URA3 gene
RC pFL40 from pFL34 & yeast 2 micron ori
RC pFL60 from pFL40 & PGK promoter/terminator & linker
RA Minet M., Lacroute F.;
RT "Cloning and sequencing of a human cDNA coding for a multifunctional
RT polypeptide of the purine pathway by complementation of the ade2-101
RT mutant in Saccharomyces cerevisiae";
RL Curr. Genet. 18:287-291(1990).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pFL40)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pFL34)(yeast 2 micron)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pFL34 ClaI 3799bp 1856..1856
FT 2. yeast 2 micron B form MstI-MstI 500bp
FT \ 3516..3942 & 703..629, ori
FT \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT -> pFL40 4299bp
FT \ [AccI, HincII, SmaI, XbaI: not unique]"
FT - 1..1857
FT /note="pFL34 1..1857 1857bp
FT ClaI = AT^CG AT
FT MstI = TGC^GCA"
FT - 1858..2284
FT /note="yeast 2 micron 3516..3942 427bp"
FT - 2285..2359
FT /note="yeast 2 micron 629..703 75bp complement
FT MstI = TGC^GCA
FT ClaI = AT^CGAT"
FT - 2360..4303
FT /note="pFL34 1856..3799 1944bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT XmaI-BamHI-XbaI"
FT misc_binding 0..0
FT /note="SIT unique NotI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
XX
SQ Sequence 4303 BP; 1121 A; 1013 C; 982 G; 1187 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag cagatctgag ctttttcttt ccaatttttt
ttttttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
ctttcgacat gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac
tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgctgcat
taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agcatcggca
tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
atacagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
aaaatgcaac gcgagctgcc gatgctcact caaaggtcgg taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//