back Return to this vector's summary.
ID   PFL44S     preliminary; circular DNA; SYN; 4319 BP.
XX
AC   X70266; ATCC77205;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pFL44S - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   632-1728
RC   pBR322-8 or pBR322-URA3 from pBR322 & S. cerevisiae URA3 gene
RC   clone 1, clone 2 from pMB9 & yeast S. cerevisiae chromosome
RC   clone 6 from pBR322 & clone 1
RC   pDURA from URA3 gene
RA   Bach M.L., Lacroute F., Botstein D.;
RT   "Evidence for transcriptional regulation of OMP-decarboxylase in
RT   yeast by hybridisation of mRNA to the yesat structural gene cloned
RT   in E. coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:386-390(1979).
XX
RN   [2]
RP   1-4319
RC   pFL44S
RA   Ozier-Kalogeropoulos O.;
RT   ;
RL   Submitted (01-JUN-1993) by:
RL   Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
XX
RN   [3]
RP   1-4319
RC   plasmid from pUC19 & linkers
RC   pFL26 from plasmid & YEp13, LEU2 gene
RC   pFL34 from plasmid & pBR322-URA3, URA3 gene
RC   pFL35 from plasmid & YRp7, TRP1 gene
RC   pFL36 from pFL26 & CEN6 & ARS
RC   pFL38 from pFL34 & CEN6 & ARS
RC   pFL39 from pFL35 & CEN6 & ARS
RC   pFL46S from pFL26 & yeast 2 micron & linker
RC   pFL44S from pFL34 & yeast 2 micron & linker
RC   pFL45S from pFL35 & yeast 2 micron & linker
RC   pFL46L from pFL26 & pFL1 & linker
RC   pFL44L from pFL34 & pFL1 & linker
RC   pFL45L from pFL35 & pFL1 & linker
RC   pFL59- from pFL39 & pGEM-3Zf-
RC   pFL59+ from pFL44S & pGEM-3Zf+
RC   pFL64- from pFL39 & pGEM-3Zf-
RC   pFL64+ from pFL44S & pGEM-3Zf+
RA   Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA   Minvielle-Sebastia L., Lacroute F.;
RT   "A family of low and high copy replicative, integrative and
RT   single-stranded S. cerevisiae/E. coli shuttle vectors";
RL   Yeast 7:609-615(1991).
XX
CC   pFL44S was constructed from pUC19 plasmid where two alu I sites
CC   were modified. The site 629 was replaced by a BglII linker and the
CC   site 747 by a ClaI site. The yeast selectable marker has been
CC   cloned in the BglII site and the 2 micron 0.5 Kb FspI fragment
CC   containing its replication origin has been cloned at the ClaI site.
CC
CC   The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC   URA3 - 2 micron ori - pMB1 ori - ampR.
CC   YE-type high copy number shuttle vector permitting visual detection of
CC   recombinants based on alpha complementation.
CC   The loss per generation (percentage having lost all the plasmids after
CC   20 h of non-selective growth) is 13.4.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.4; BglII--3.3, 1.1; ClaI--3.9, 0.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 397126
CC   NM (pFL44S)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(yeast 2-micron plasmid)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFL34 ClaI 3799bp 1856..1856
FT                   2. yeast 2 micron XbaI 6318bp 3946..3946
FT                   fill in
FT                   FspI-FspI 509bp 3516..4025, ori
FT                   ClaI linker 6bp atcgat:ClaI linker 6bp atcgat
FT                   -> pFL44S 4319bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT                   XmaI-BamHI-XbaI-
FT                   AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT                   XbaI-SalI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 4319 BP; 1125 A; 1016 C; 984 G; 1194 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag cagatctgag ctttttcttt ccaatttttt
     ttttttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
     cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
     catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
     ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
     caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
     caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
     attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
     gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
     caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
     gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
     acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
     tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
     attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
     tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
     ctttcgacat gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac
     tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
     ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgctgcat
     taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agcatcgatg
     catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
     acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
     gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
     agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
     ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
     caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
     ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
     ctatactttc tagctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
     acgagcgctt ccgaaaatgc aacgcgagct gccatcgatg ctcactcaaa ggtcggtaat
     acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//