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ID   PFR109     preliminary; circular DNA; SYN; 9898 BP.
XX
AC   IG0042; K01454;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pFR109 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-95
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   NM (pFR109)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMC1403)(pBR322)
CC   BR (pFR97)(pFR98)(pFR400)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFR10 PstI-PstI, MCS/amp gene
FT                   2. pSKS106 remove PstI-PstI, MCS/amp gene
FT                   -> pFR109 9898bp [unique XhoI-XbaI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   CDS             3798..6852
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             6903..8157
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             8220..8832
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 9898 BP; 2317 A; 2650 C; 2588 G; 2343 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt ttgacagctt
     atcatcggag ctctcgagtc tagaatcgat aagcttggct gcaggtcgac ggatccccgg
     gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
     ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
     cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac
     ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
     gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
     tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
     cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
     cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg
     gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
     tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
     tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
     aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
     ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
     aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta
     tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat
     gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag
     tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt
     tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta ttaccggcat
     gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac aatacaacat
     tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg ccggtgcgcc
     agcagtagag gcatttattg agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc
     gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca tcatgttcac
     catcaataat cagtttgttt tctggctggg ctctggctgt gcactcatcc tcgccgtttt
     actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg cggtaggtgc
     caaccattcg gcatttagcc ttaagctggc actggaactg ttcagacagc caaaactgtg
     gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg accaacagtt
     tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg tatttggcta
     cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc cactgatcat
     taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt ctgtacgtat
     tattggctca tcgttcgcca cctcagcgct ggaagtggtt attctgaaaa cgctgcatat
     gtttgaagta ccgttcctgc tggtgggctg ctttaaatat attaccagcc agtttgaagt
     gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac tggcgatgat
     ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg gcgcttatct
     ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc ttagcggccc
     cggcccgctt tccctgctgc gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg
     atgcggcgcg acgcttatcc gaccaacata tcataacgga gtgatcgcat tgaacatgcc
     aatgaccgaa agaataagag caggcaagct atttaccgat atgtgcgaag gcttaccgga
     aaaaagactt cgtgggaaaa cgttaatgta tgagtttaat cactcgcatc catcagaagt
     tgaaaaaaga gaaagcctga ttaaagaaat gtttgccacg gtaggggaaa acgcctgggt
     agaaccgcct gtctatttct cttacggttc caacatccat ataggccgca atttttatgc
     aaatttcaat ttaaccattg tcgatgacta cacggtaaca atcggtgata acgtactgat
     tgcacccaac gttactcttt ccgttacggg acaccctgta caccatgaat tgagaaaaaa
     cggcgagatg tactcttttc cgataacgat tggcaataac gtctggatcg gaagtcatgt
     ggttattaat ccaggcgtca ccatcgggga taattctgtt attggcgcgg gtagtatcgt
     cacaaaagac attccaccaa acgtcgtggc ggctggcgtt ccttgtcggg ttattcgcga
     aataaacgac cgggataagc actattattt caaagattat aaagttgaat cgtcagttta
     aattataaaa attgcctgat acgctgcgct tatcaggcct acaagttcag cgatctacat
     tagccgcatc cggcatgaac aaagcgcagg aacaagcgtc gcatcatgcc tctttgaccc
     acagctgcgg aaaacgtact ggtgcaaaac gcagggttat gatcatcagc ccaacgacgc
     acagcgcatg aaatgcccag tccatcaggt aattgccgct gatactacgc agcacgccag
     aaaaccacgg ggcaagcccg gcgatgataa aaccgattcc ctgcataaac gccaccagct
     tgccagcaat agccggttgc acagagtgat cgagcgccag cagcaaacag agcggaaacg
     cgccgcccag acctaaccca cacaccatcg cccacaatac cggcaattgc atcggcagcc
     agataaagcc gcagaacccc accagttgta acaccagcgc cagcattaac agtttgcgcc
     gatcctgatg gcgagccata gcaggcatca gcaaagctcc tgcggcttgc ccaagcgtca
     tcaatgccag taaggaaccg ctgtactgcg cgctggcacc aatctcaata tagaaagcgg
     gtaaccaggc aatcaggctg gcgtaaccgc cgttaatcag accgaagtaa acacccagcg
     tccacgcgcg gggagtgaat accacgcgaa ccggagtggt tgttgtcttg tgggaagagg
     cgacctcgcg ggcgctttgc caccaccagg caaagagcgc aacaacggca ggcagcgcca
     ccaggcgagt gtttgatacc aggtttcgct atgttgaact aaccagggcg ttatggcggc
     accaagccca ccgccgccca tcagagccgc ggaccacagc cccatcacca gtggcgtgcg
     ctgctgaaac cgccgtttaa tcaccgaagc atcaccgcct gaatgatgcc gatccccacc
     ccaccaagca gtgcgctgct aagcagcagc gcactttgcg ggtaaagctc acgcatcaat
     gcaccgacgg caatcagcaa cagactgatg gcgacactgc gacgttcgct gacatgctga
     tgaagccagc ttccggccag cgccagcccg cccatggtaa ccaccggcag agcggtaa
//