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ID   PFR97      preliminary; circular DNA; SYN; 9894 BP.
XX
AC   IG0043; K01452;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pFR97 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-91
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   NM (pFR97)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMC1403)(pBR322)
CC   BR (pFR109)(pFR98)(pFR400)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFR10 PstI-PstI, MCS/amp gene
FT                   2. pSKS104 remove PstI-PstI, MCS/amp gene
FT                   -> pFR97 9894bp [unique XhoI-XbaI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   CDS             3794..6848
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             8216..8826
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             6898..8152
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 9894 BP; 2316 A; 2647 C; 2589 G; 2342 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt ttgacagctt
     atcggagctc tcgagtctag aatcgataag cttggctgca ggtcgacgga tccggggaat
     tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
     cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
     acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
     cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
     gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg
     agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
     catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
     atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
     caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
     ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
     gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
     cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
     tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc
     ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
     tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
     ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg
     gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac
     tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac
     tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag
     caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
     gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     gtgatgtttg cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta
     gtaggatcga ttgttggtgg tatttatcta ggcttttgtt ttaacgccgg tgcgccagca
     gtagaggcat ttattgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg
     atgtttggct gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc
     aataatcagt ttgttttctg gctgggctct ggctgtgcac tcatcctcgc cgttttactc
     tttttcgcca aaacggatgc gccctcttct gccacggttg ccaatgcggt aggtgccaac
     cattcggcat ttagccttaa gctggcactg gaactgttca gacagccaaa actgtggttt
     ttgtcactgt atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct
     aatttcttta cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta
     acgacaatgg gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat
     cgcatcggtg ggaaaaacgc cctgctgctg gctggcacta ttatgtctgt acgtattatt
     ggctcatcgt tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt
     gaagtaccgt tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt
     ttttcagcga cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt
     atgtctgtac tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg
     ctgggtctgg tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc
     ccgctttccc tgctgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc
     ggcgcgacgc ttatccgacc aacatatcat aacggagtga tcgcattgaa catgccaatg
     accgaaagaa taagagcagg caagctattt accgatatgt gcgaaggctt accggaaaaa
     agacttcgtg ggaaaacgtt aatgtatgag tttaatcact cgcatccatc agaagttgaa
     aaaagagaaa gcctgattaa agaaatgttt gccacggtag gggaaaacgc ctgggtagaa
     ccgcctgtct atttctctta cggttccaac atccatatag gccgcaattt ttatgcaaat
     ttcaatttaa ccattgtcga tgactacacg gtaacaatcg gtgataacgt actgattgca
     cccaacgtta ctctttccgt tacgggacac cctgtacacc atgaattgag aaaaaacggc
     gagatgtact cttttccgat aacgattggc aataacgtct ggatcggaag tcatgtggtt
     attaatccag gcgtcaccat cggggataat tctgttattg gcgcgggtag tatcgtcaca
     aaagacattc caccaaacgt cgtggcggct ggcgttcctt gtcgggttat tcgcgaaata
     aacgaccggg ataagcacta ttatttcaaa gattataaag ttgaatcgtc agtttaaatt
     ataaaaattg cctgatacgc tgcgcttatc aggcctacaa gttcagcgat ctacattagc
     cgcatccggc atgaacaaag cgcaggaaca agcgtcgcat catgcctctt tgacccacag
     ctgcggaaaa cgtactggtg caaaacgcag ggttatgatc atcagcccaa cgacgcacag
     cgcatgaaat gcccagtcca tcaggtaatt gccgctgata ctacgcagca cgccagaaaa
     ccacggggca agcccggcga tgataaaacc gattccctgc ataaacgcca ccagcttgcc
     agcaatagcc ggttgcacag agtgatcgag cgccagcagc aaacagagcg gaaacgcgcc
     gcccagacct aacccacaca ccatcgccca caataccggc aattgcatcg gcagccagat
     aaagccgcag aaccccacca gttgtaacac cagcgccagc attaacagtt tgcgccgatc
     ctgatggcga gccatagcag gcatcagcaa agctcctgcg gcttgcccaa gcgtcatcaa
     tgccagtaag gaaccgctgt actgcgcgct ggcaccaatc tcaatataga aagcgggtaa
     ccaggcaatc aggctggcgt aaccgccgtt aatcagaccg aagtaaacac ccagcgtcca
     cgcgcgggga gtgaatacca cgcgaaccgg agtggttgtt gtcttgtggg aagaggcgac
     ctcgcgggcg ctttgccacc accaggcaaa gagcgcaaca acggcaggca gcgccaccag
     gcgagtgttt gataccaggt ttcgctatgt tgaactaacc agggcgttat ggcggcacca
     agcccaccgc cgcccatcag agccgcggac cacagcccca tcaccagtgg cgtgcgctgc
     tgaaaccgcc gtttaatcac cgaagcatca ccgcctgaat gatgccgatc cccaccccac
     caagcagtgc gctgctaagc agcagcgcac tttgcgggta aagctcacgc atcaatgcac
     cgacggcaat cagcaacaga ctgatggcga cactgcgacg ttcgctgaca tgctgatgaa
     gccagcttcc ggccagcgcc agcccgccca tggtaaccac cggcagagcg gtaa
//