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ID   PFR98      preliminary; circular DNA; SYN; 9887 BP.
XX
AC   IG0044; K01453;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pFR98 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-91
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   NM (pFR98)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMC1403)(pBR322)
CC   BR (pFR97)(pFR109)(pFR400)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pFR10 PstI-PstI, MCS/amp gene
FT                   2. pSKS105 remove PstI-PstI, MCS/amp gene
FT                   -> pFR98 9887bp [unique XhoI-XbaI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   CDS             3787..6841
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             8119..8821
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             6892..8146
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 9887 BP; 2315 A; 2647 C; 2584 G; 2341 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt ttgacagctt
     atcatcggag ctctcgagtc tagaatcgat aagcttggct gcagccaagc ttggcactgg
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
     agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
     gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
     caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
     tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
     ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
     ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
     ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat
     cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg
     catttattga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg
     gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc
     agtttgtttt ctggctgggc tctggctgtg cactcatcct cgccgtttta ctctttttcg
     ccaaaacgga tgcgccctct tctgccacgg ttgccaatgc ggtaggtgcc aaccattcgg
     catttagcct taagctggca ctggaactgt tcagacagcc aaaactgtgg tttttgtcac
     tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct
     ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa
     tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg
     gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat
     cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac
     cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag
     cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg
     tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc
     tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt
     ccctgctgcg tcgtcaggtg aatgaagtcg cttaagcaat caatgtcgga tgcggcgcga
     cgcttatccg accaacatat cataacggag tgatcgcatt gaacatgcca atgaccgaaa
     gaataagagc aggcaagcta tttaccgata tgtgcgaagg cttaccggaa aaaagacttc
     gtgggaaaac gttaatgtat gagtttaatc actcgcatcc atcagaagtt gaaaaaagag
     aaagcctgat taaagaaatg tttgccacgg taggggaaaa cgcctgggta gaaccgcctg
     tctatttctc ttacggttcc aacatccata taggccgcaa tttttatgca aatttcaatt
     taaccattgt cgatgactac acggtaacaa tcggtgataa cgtactgatt gcacccaacg
     ttactctttc cgttacggga caccctgtac accatgaatt gagaaaaaac ggcgagatgt
     actcttttcc gataacgatt ggcaataacg tctggatcgg aagtcatgtg gttattaatc
     caggcgtcac catcggggat aattctgtta ttggcgcggg tagtatcgtc acaaaagaca
     ttccaccaaa cgtcgtggcg gctggcgttc cttgtcgggt tattcgcgaa ataaacgacc
     gggataagca ctattatttc aaagattata aagttgaatc gtcagtttaa attataaaaa
     ttgcctgata cgctgcgctt atcaggccta caagttcagc gatctacatt agccgcatcc
     ggcatgaaca aagcgcagga acaagcgtcg catcatgcct ctttgaccca cagctgcgga
     aaacgtactg gtgcaaaacg cagggttatg atcatcagcc caacgacgca cagcgcatga
     aatgcccagt ccatcaggta attgccgctg atactacgca gcacgccaga aaaccacggg
     gcaagcccgg cgatgataaa accgattccc tgcataaacg ccaccagctt gccagcaata
     gccggttgca cagagtgatc gagcgccagc agcaaacaga gcggaaacgc gccgcccaga
     cctaacccac acaccatcgc ccacaatacc ggcaattgca tcggcagcca gataaagccg
     cagaacccca ccagttgtaa caccagcgcc agcattaaca gtttgcgccg atcctgatgg
     cgagccatag caggcatcag caaagctcct gcggcttgcc caagcgtcat caatgccagt
     aaggaaccgc tgtactgcgc gctggcacca atctcaatat agaaagcggg taaccaggca
     atcaggctgg cgtaaccgcc gttaatcaga ccgaagtaaa cacccagcgt ccacgcgcgg
     ggagtgaata ccacgcgaac cggagtggtt gttgtcttgt gggaagaggc gacctcgcgg
     gcgctttgcc accaccaggc aaagagcgca acaacggcag gcagcgccac caggcgagtg
     tttgatacca ggtttcgcta tgttgaacta accagggcgt tatggcggca ccaagcccac
     cgccgcccat cagagccgcg gaccacagcc ccatcaccag tggcgtgcgc tgctgaaacc
     gccgtttaat caccgaagca tcaccgcctg aatgatgccg atccccaccc caccaagcag
     tgcgctgcta agcagcagcg cactttgcgg gtaaagctca cgcatcaatg caccgacggc
     aatcagcaac agactgatgg cgacactgcg acgttcgctg acatgctgat gaagccagct
     tccggccagc gccagcccgc ccatggtaac caccggcaga gcggtaa
//