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ID   PGAD10     preliminary; circular DNA; SYN; 6650 BP.
XX
AC   U13188;
XX
DT   28-AUG-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli vector pGAD10 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6650
RC   pGAD10 from ColE1 & GAL4 & LEU2 gene
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.2";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-6650
RC   pGAD10
RA   Kitts P.A.;
RT   ;
RL   Submitted (08-AUG-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   NCBI gi: 532698
CC   NM (pGAD10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1, ori/amp gene
FT                   2. yeast, GAL4 promoter
FT                   3. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> pGAD10 6650bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6650 BP; 1901 A; 1496 C; 1362 G; 1891 T; 0 other;
     gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
     atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
     cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
     tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
     tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
     cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
     ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
     gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
     taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
     cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
     acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
     taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
     gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
     taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctctcgagg
     atccgaattc cagatctatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact
     gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt cttttatgta
     actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa
     atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac
     gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcccgaaa ttcccctacc
     ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggtcaacctt
     attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
     cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct catctggaag
     tgggacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt
     aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact
     tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg
     gttccggcct ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt
     caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag
     cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac
     tcttgcatct tacgatacct gagtattccc acagttgggg atctcgactc tagctagagg
     atcaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg
     taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     cagctggatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccataacgca tttaagcata aacacgcact atgccgttct
     tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
     gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
     tcctattccg aagttcctat tctctagcta gaaagtatag gaacttcaga gcgcttttga
     aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg
     agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct
     atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt
     gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa
     aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt
     tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg
     aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag
     aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag
     taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc
     cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag
     cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga
     tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc
     gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct
     ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
//