back Return to this vector's summary.
ID   PGAL184    preliminary; circular DNA; SYN; 10198 BP.
XX
AC   IG9918;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGal184 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGal184 from pACYC184 & pMC1403
RC   pXM184LacA from pTrc99A
RC   pXM184LacB from pTrc99B
RC   pXM184LacC from pTrc99C
RC   pGal184Z from pGal184
RC   pXM184Z.A from pGal184Z & pTrc99A
RC   pXM184Z.B from pGal184Z & pTrc99B
RC   pXM184Z.C from pGal184Z & pTrc99C
RA   Bingham A.H.;
RT   "Low copy number vectors for expression of fused genes to
RT   beta-galactosidase in Escherichia coli";
RL   FEMS Microbiol. Lett. 79:239-246(1991).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pGal184)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC184)(pMC1403)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove BamHI-SalI 276bp 1871..2147,
FT                   \ 3969bp
FT                   2. pMC1403 BamHI-SalI 6229bp 3650..9820..59, lacZYA
FT                   -> pGal184 10198bp"
FT   -               1..1870
FT                   /note="pACYC184 1..1870 1870bp
FT                   BamHI = G^GATCC"
FT   -               1871..8099
FT                   /note="pMC1403 3650..9820..58 6229bp
FT                   SalI = G^TCGAC"
FT   -               8100..10198
FT                   /note="pACYC184 2147..4245 2099bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 10198 BP; 2365 A; 2738 C; 2650 G; 2445 T; 0 other;
     gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     ccgtcctgtg gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
     tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
     tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
     aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
     ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
     tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
     ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
     cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg
     gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
     cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
     agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg
     tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg
     tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc
     cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat
     tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct
     gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca
     tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa
     ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga
     ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat
     gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat
     ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg
     ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
     ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
     cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
     gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg
     taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
     gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
     cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
     gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca
     gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
     gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
     ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
     tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
     gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
     ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
     cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
     ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
     gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
     taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
     gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
     gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
     aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
     cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
     agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
     agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga
     attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag
     tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg
     gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt
     atcggcggaa tttcagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa
     ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta
     ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat
     catgggagcc tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa
     aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct
     gtttggtctg ctttctgaca aactcgggct gcgcaaatac ctgctgtgga ttattaccgg
     catgttagtg atgtttgcgc cgttctttat ttttatcttc gggccactgt tacaatacaa
     cattttagta ggatcgattg ttggtggtat ttatctaggc ttttgtttta acgccggtgc
     gccagcagta gaggcattta ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg
     cgcgcggatg tttggctgtg ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt
     caccatcaat aatcagtttg ttttctggct gggctctggc tgtgcactca tcctcgccgt
     tttactcttt ttcgccaaaa cggatgcgcc ctcttctgcc acggttgcca atgcggtagg
     tgccaaccat tcggcattta gccttaagct ggcactggaa ctgttcagac agccaaaact
     gtggtttttg tcactgtatg ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca
     gtttgctaat ttctttactt cgttctttgc taccggtgaa cagggtacgc gggtatttgg
     ctacgtaacg acaatgggcg aattacttaa cgcctcgatt atgttctttg cgccactgat
     cattaatcgc atcggtggga aaaacgccct gctgctggct ggcactatta tgtctgtacg
     tattattggc tcatcgttcg ccacctcagc gctggaagtg gttattctga aaacgctgca
     tatgtttgaa gtaccgttcc tgctggtggg ctgctttaaa tatattacca gccagtttga
     agtgcgtttt tcagcgacga tttatctggt ctgtttctgc ttctttaagc aactggcgat
     gatttttatg tctgtactgg cgggcaatat gtatgaaagc atcggtttcc agggcgctta
     tctggtgctg ggtctggtgg cgctgggctt caccttaatt tccgtgttca cgcttagcgg
     ccccggcccg ctttccctgc tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt
     cggatgcggc gcgacgctta tccgaccaac atatcataac ggagtgatcg cattgaacat
     gccaatgacc gaaagaataa gagcaggcaa gctatttacc gatatgtgcg aaggcttacc
     ggaaaaaaga cttcgtggga aaacgttaat gtatgagttt aatcactcgc atccatcaga
     agttgaaaaa agagaaagcc tgattaaaga aatgtttgcc acggtagggg aaaacgcctg
     ggtagaaccg cctgtctatt tctcttacgg ttccaacatc catataggcc gcaattttta
     tgcaaatttc aatttaacca ttgtcgatga ctacacggta acaatcggtg ataacgtact
     gattgcaccc aacgttactc tttccgttac gggacaccct gtacaccatg aattgagaaa
     aaacggcgag atgtactctt ttccgataac gattggcaat aacgtctgga tcggaagtca
     tgtggttatt aatccaggcg tcaccatcgg ggataattct gttattggcg cgggtagtat
     cgtcacaaaa gacattccac caaacgtcgt ggcggctggc gttccttgtc gggttattcg
     cgaaataaac gaccgggata agcactatta tttcaaagat tataaagttg aatcgtcagt
     ttaaattata aaaattgcct gatacgctgc gcttatcagg cctacaagtt cagcgatcta
     cattagccgc atccggcatg aacaaagcgc aggaacaagc gtcgcatcat gcctctttga
     cccacagctg cggaaaacgt actggtgcaa aacgcagggt tatgatcatc agcccaacga
     cgcacagcgc atgaaatgcc cagtccatca ggtaattgcc gctgatacta cgcagcacgc
     cagaaaacca cggggcaagc ccggcgatga taaaaccgat tccctgcata aacgccacca
     gcttgccagc aatagccggt tgcacagagt gatcgagcgc cagcagcaaa cagagcggaa
     acgcgccgcc cagacctaac ccacacacca tcgcccacaa taccggcaat tgcatcggca
     gccagataaa gccgcagaac cccaccagtt gtaacaccag cgccagcatt aacagtttgc
     gccgatcctg atggcgagcc atagcaggca tcagcaaagc tcctgcggct tgcccaagcg
     tcatcaatgc cagtaaggaa ccgctgtact gcgcgctggc accaatctca atatagaaag
     cgggtaacca ggcaatcagg ctggcgtaac cgccgttaat cagaccgaag taaacaccca
     gcgtccacgc gcggggagtg aataccacgc gaaccggagt ggttgttgtc ttgtgggaag
     aggcgacctc gcgggcgctt tgccaccacc aggcaaagag cgcaacaacg gcaggcagcg
     ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg gcgttatggc
     ggcaccaagc ccaccgccgc ccatcagagc cgcggaccac agccccatca ccagtggcgt
     gcgctgctga aaccgccgtt taatcaccga agcatcaccg cctgaatgat gccgatcccc
     accccaccaa gcagtgcgct gctaagcagc agcgcacttt gcgggtaaag ctcacgcatc
     aatgcaccga cggcaatcag caacagactg atggcgacac tgcgacgttc gctgacatgc
     tgatgaagcc agcttccggc cagcgccagc ccgcccatgg taaccaccgg cagagcggta
     accgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctt
     cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
     gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     gtgtttcgta aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac
     agagagtgga accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg
     gcctcatttc ttattctgag ttacaacagt ccgcaccgct gtccggtagc tccttccggt
     gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     taggacaggt gccggcagcg cccaacagtc ccccggccac ggggcctgcc accataccca
     cgccgaaaca agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
     taccctgtgg aacacctaca tctgtattaa cgaagcgcta accgttttta tcaggctctg
     ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa
     ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt
     aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc
     gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac
     caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
     gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacg
//